{"id":"51e49691-4d19-5ecd-be45-41fb961f7270","stable_key":"27e0c1cf-7726-5164-8b15-27a631584cd0:choline-slc25a48-purines","predicate":"reduces","statement":"SLC25A48 loss reduced the contribution of labeled choline to purine nucleotides in HEK293T cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"7e74d0de-79e9-5721-8c60-7413b352fd01","mechanism_event_label":"Choline-derived metabolism reached nucleotide production in the tracer experiment.","subject":{"id":"513ac047-ea0d-52cd-9e44-3fbb5779a035","slug":"human-slc25a48-deficient-cells","display_name":"SLC25A48-deficient human cells in the 2024 transport studies","entity_type_key":"protein_state"},"object":{"id":"1ae2de52-4bee-5861-ab25-6c32b1753d61","slug":"human-choline-derived-purine-labeling","display_name":"Human HEK293T choline-derived purine nucleotide labeling","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"7e74d0de-79e9-5721-8c60-7413b352fd01","stable_key":"27e0c1cf-7726-5164-8b15-27a631584cd0:choline-slc25a48-purines-event","event_type":"biochemical_relationship","label":"Choline-derived metabolism reached nucleotide production in the tracer experiment.","description":"SLC25A48 loss reduced the contribution of labeled choline to purine nucleotides in HEK293T cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"513ac047-ea0d-52cd-9e44-3fbb5779a035","slug":"human-slc25a48-deficient-cells","display_name":"SLC25A48-deficient human cells in the 2024 transport studies","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1ae2de52-4bee-5861-ab25-6c32b1753d61","slug":"human-choline-derived-purine-labeling","display_name":"Human HEK293T choline-derived purine nucleotide labeling","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/choline-research/39111307.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"c0a9377732782bdaa55105cae0a0446afcfb1225e686d2b047a7fdf1ef67535f\", \"start_char\": 0, \"end_char\": 1201, \"text_sha256\": \"c0a9377732782bdaa55105cae0a0446afcfb1225e686d2b047a7fdf1ef67535f\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Slc25a48 knockout, rescue, mitochondrial assays and isotope tracing","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"1 mM 13C2-choline for 24 hours in the study methods","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Tracer contribution is not a claim that choline directly becomes a whole nucleotide or that dietary choline controls every purine pathway.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Choline research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"choline","display_name":"Choline","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human HEK293T cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Choline-derived metabolism reached nucleotide production in the tracer experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[choline-p39111307] SLC25A48 controls mitochondrial choline import and metabolism. (2024). https://pubmed.ncbi.nlm.nih.gov/39111307/ DOI: 10.1016/j.cmet.2024.07.010","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Brown adipose tissue and human HEK293T cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"ac73b844-a374-5414-acb6-8c412195d85a","evidence_kind":"source_excerpt","locator":"Lines 503-514","start_line":503,"end_line":514,"excerpt":"### choline-slc25a48-purines\nSLC25A48 loss reduced the contribution of labeled choline to purine nucleotides in HEK293T cells.\nCondition category: machinery_impairment\nnutrient_topic: Choline research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Choline-derived metabolism reached nucleotide production in the tracer experiment.\norganism: Human HEK293T cells\ntissue_or_cell_type: Brown adipose tissue and human HEK293T cells\nexperimental_model: Slc25a48 knockout, rescue, mitochondrial assays and isotope tracing\nlimitations: Tracer contribution is not a claim that choline directly becomes a whole nucleotide or that dietary choline controls every purine pathway.\nexposure: 1 mM 13C2-choline for 24 hours in the study methods\nevidence_span: {\"source_cache\": \"artifacts/choline-research/39111307.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"c0a9377732782bdaa55105cae0a0446afcfb1225e686d2b047a7fdf1ef67535f\", \"start_char\": 0, \"end_char\": 1201, \"text_sha256\": \"c0a9377732782bdaa55105cae0a0446afcfb1225e686d2b047a7fdf1ef67535f\"}\n[choline-p39111307] SLC25A48 controls mitochondrial choline import and metabolism. 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