Component

Human monocarboxylate transporter SLC16A11

6 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

Where it participates (unsigned role)

  1. The published human K562 dual-targeting screen reports SLC16A11/SLC25A39 genetic interaction pi=0.0805858670811976 with significance value 0.612290402312043 under Anti15. This is a relative-fitness interaction estimate, not restoration of mitochondrial glutathione.

    Combined SLC16A11 and SLC25A39 targeting in human K562 screen → Human K562 pooled screen relative fitness source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    machinery_impairment Imported condition classification; unverified.
    experimental_model
    Human K562 pooled CRISPR screen; two batches; paired SaCas9/SpCas9 guides
    exposure
    15-day growth screen; 25 mM glucose, 1 mM sodium pyruvate, 100 nM antimycin; 10% dialyzed FBS and 50 microgram/mL uridine.
    limitations
    Positive pi describes departure from an additive fitness expectation, not positive regulation between wild-type proteins. All four pi estimates are below the authors absolute pi >0.25 large-effect cutoff. Glucose significance value is below 0.02; other conditions are not. These are authors reported BH-adjusted values according to their archived pi_score code, not new p-values. Guide constructs are not independent biological replicates. No new genotype-by-environment significance test was performed.
    organism
    Human
    primary_locator
    Supplementary Data 3, Anti15_piscore and Anti15_pvalue, BD40 (row SLC25A39, column SLC16A11); Supplementary Data 4 All_Data_Sig_0.02 includes the glucose pair.
    primary_references
    https://doi.org/10.1038/s41467-022-30126-9
    trigger_kind
    machinery_impairment Imported condition classification; unverified.

    SLC16A11 and mitochondrial glutathione: published screen connection and transport evidence · lines 24–30

    Primary sources 2022 and 2017, plus labelled descriptive reanalysis on 2026-09-20. · supports · Human K562 pooled CRISPR screen; two batches; paired SaCas9/SpCas9 guides · source_derived_draft · unverified_draft

    The published human K562 dual-targeting screen reports SLC16A11/SLC25A39 genetic interaction pi=0.0805858670811976 with significance value 0.612290402312043 under Anti15. This is a relative-fitness interaction estimate, not restoration of mitochondrial glutathione. primary_references: https://doi.org/10.1038/s41467-022-30126-9 primary_locator: Supplementary Data 3, Anti15_piscore and Anti15_pvalue, BD40 (row SLC25A39, column SLC16A11); Supplementary Data 4 All_Data_Sig_0.02 includes the glucose pair. organism: Human experimental_model: Human K562 pooled CRISPR screen; two batches; paired SaCas9/SpCas9 guides exposure: 15-day growth screen; 25 mM glucose, 1 mM sodium pyruvate, 100 nM antimycin; 10% dialyzed FBS and 50 microgram/mL uridine. limitations: Positive pi describes departure from an additive fitness expectation, not positive regulation between wild-type proteins. All four pi estimates are below the authors absolute pi >0.25 large-effect cutoff. Glucose significance value is below 0.02; other conditions are not. These are authors reported BH-adjusted values according to their archived pi_score code, not new p-values. Guide constructs are not independent biological replicates. No new genotype-by-environment significance test was performed.
    Complete structured claim and evidence

  2. The published human K562 dual-targeting screen reports SLC16A11/SLC25A39 genetic interaction pi=0.0272123713337914 with significance value 0.578178616323455 under Gal15. This is a relative-fitness interaction estimate, not restoration of mitochondrial glutathione.

    Combined SLC16A11 and SLC25A39 targeting in human K562 screen → Human K562 pooled screen relative fitness source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    machinery_impairment Imported condition classification; unverified.
    experimental_model
    Human K562 pooled CRISPR screen; two batches; paired SaCas9/SpCas9 guides
    exposure
    15-day growth screen; 25 mM galactose replacing glucose, 1 mM sodium pyruvate; 10% dialyzed FBS and 50 microgram/mL uridine.
    limitations
    Positive pi describes departure from an additive fitness expectation, not positive regulation between wild-type proteins. All four pi estimates are below the authors absolute pi >0.25 large-effect cutoff. Glucose significance value is below 0.02; other conditions are not. These are authors reported BH-adjusted values according to their archived pi_score code, not new p-values. Guide constructs are not independent biological replicates. No new genotype-by-environment significance test was performed.
    organism
    Human
    primary_locator
    Supplementary Data 3, Gal15_piscore and Gal15_pvalue, BD40 (row SLC25A39, column SLC16A11); Supplementary Data 4 All_Data_Sig_0.02 includes the glucose pair.
    primary_references
    https://doi.org/10.1038/s41467-022-30126-9
    trigger_kind
    machinery_impairment Imported condition classification; unverified.

    SLC16A11 and mitochondrial glutathione: published screen connection and transport evidence · lines 15–21

    Primary sources 2022 and 2017, plus labelled descriptive reanalysis on 2026-09-20. · supports · Human K562 pooled CRISPR screen; two batches; paired SaCas9/SpCas9 guides · source_derived_draft · unverified_draft

    The published human K562 dual-targeting screen reports SLC16A11/SLC25A39 genetic interaction pi=0.0272123713337914 with significance value 0.578178616323455 under Gal15. This is a relative-fitness interaction estimate, not restoration of mitochondrial glutathione. primary_references: https://doi.org/10.1038/s41467-022-30126-9 primary_locator: Supplementary Data 3, Gal15_piscore and Gal15_pvalue, BD40 (row SLC25A39, column SLC16A11); Supplementary Data 4 All_Data_Sig_0.02 includes the glucose pair. organism: Human experimental_model: Human K562 pooled CRISPR screen; two batches; paired SaCas9/SpCas9 guides exposure: 15-day growth screen; 25 mM galactose replacing glucose, 1 mM sodium pyruvate; 10% dialyzed FBS and 50 microgram/mL uridine. limitations: Positive pi describes departure from an additive fitness expectation, not positive regulation between wild-type proteins. All four pi estimates are below the authors absolute pi >0.25 large-effect cutoff. Glucose significance value is below 0.02; other conditions are not. These are authors reported BH-adjusted values according to their archived pi_score code, not new p-values. Guide constructs are not independent biological replicates. No new genotype-by-environment significance test was performed.
    Complete structured claim and evidence

  3. The published human K562 dual-targeting screen reports SLC16A11/SLC25A39 genetic interaction pi=0.154314615559463 with significance value 0.0185030434905457 under Glc15. This is a relative-fitness interaction estimate, not restoration of mitochondrial glutathione.

    Combined SLC16A11 and SLC25A39 targeting in human K562 screen → Human K562 pooled screen relative fitness source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    machinery_impairment Imported condition classification; unverified.
    experimental_model
    Human K562 pooled CRISPR screen; two batches; paired SaCas9/SpCas9 guides
    exposure
    15-day growth screen; 25 mM glucose, 1 mM sodium pyruvate; 10% dialyzed FBS and 50 microgram/mL uridine.
    limitations
    Positive pi describes departure from an additive fitness expectation, not positive regulation between wild-type proteins. All four pi estimates are below the authors absolute pi >0.25 large-effect cutoff. Glucose significance value is below 0.02; other conditions are not. These are authors reported BH-adjusted values according to their archived pi_score code, not new p-values. Guide constructs are not independent biological replicates. No new genotype-by-environment significance test was performed.
    organism
    Human
    primary_locator
    Supplementary Data 3, Glc15_piscore and Glc15_pvalue, BD40 (row SLC25A39, column SLC16A11); Supplementary Data 4 All_Data_Sig_0.02 includes the glucose pair.
    primary_references
    https://doi.org/10.1038/s41467-022-30126-9
    trigger_kind
    machinery_impairment Imported condition classification; unverified.

    SLC16A11 and mitochondrial glutathione: published screen connection and transport evidence · lines 6–12

    Primary sources 2022 and 2017, plus labelled descriptive reanalysis on 2026-09-20. · supports · Human K562 pooled CRISPR screen; two batches; paired SaCas9/SpCas9 guides · source_derived_draft · unverified_draft

    The published human K562 dual-targeting screen reports SLC16A11/SLC25A39 genetic interaction pi=0.154314615559463 with significance value 0.0185030434905457 under Glc15. This is a relative-fitness interaction estimate, not restoration of mitochondrial glutathione. primary_references: https://doi.org/10.1038/s41467-022-30126-9 primary_locator: Supplementary Data 3, Glc15_piscore and Glc15_pvalue, BD40 (row SLC25A39, column SLC16A11); Supplementary Data 4 All_Data_Sig_0.02 includes the glucose pair. organism: Human experimental_model: Human K562 pooled CRISPR screen; two batches; paired SaCas9/SpCas9 guides exposure: 15-day growth screen; 25 mM glucose, 1 mM sodium pyruvate; 10% dialyzed FBS and 50 microgram/mL uridine. limitations: Positive pi describes departure from an additive fitness expectation, not positive regulation between wild-type proteins. All four pi estimates are below the authors absolute pi >0.25 large-effect cutoff. Glucose significance value is below 0.02; other conditions are not. These are authors reported BH-adjusted values according to their archived pi_score code, not new p-values. Guide constructs are not independent biological replicates. No new genotype-by-environment significance test was performed.
    Complete structured claim and evidence

  4. The published human K562 dual-targeting screen reports SLC16A11/SLC25A39 genetic interaction pi=0.155012680942449 with significance value 0.0621187730457448 under Pyr15. This is a relative-fitness interaction estimate, not restoration of mitochondrial glutathione.

    Combined SLC16A11 and SLC25A39 targeting in human K562 screen → Human K562 pooled screen relative fitness source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    machinery_impairment Imported condition classification; unverified.
    experimental_model
    Human K562 pooled CRISPR screen; two batches; paired SaCas9/SpCas9 guides
    exposure
    15-day growth screen; 25 mM glucose, without added sodium pyruvate; 10% dialyzed FBS and 50 microgram/mL uridine.
    limitations
    Positive pi describes departure from an additive fitness expectation, not positive regulation between wild-type proteins. All four pi estimates are below the authors absolute pi >0.25 large-effect cutoff. Glucose significance value is below 0.02; other conditions are not. These are authors reported BH-adjusted values according to their archived pi_score code, not new p-values. Guide constructs are not independent biological replicates. No new genotype-by-environment significance test was performed.
    organism
    Human
    primary_locator
    Supplementary Data 3, Pyr15_piscore and Pyr15_pvalue, BD40 (row SLC25A39, column SLC16A11); Supplementary Data 4 All_Data_Sig_0.02 includes the glucose pair.
    primary_references
    https://doi.org/10.1038/s41467-022-30126-9
    trigger_kind
    machinery_impairment Imported condition classification; unverified.

    SLC16A11 and mitochondrial glutathione: published screen connection and transport evidence · lines 33–39

    Primary sources 2022 and 2017, plus labelled descriptive reanalysis on 2026-09-20. · supports · Human K562 pooled CRISPR screen; two batches; paired SaCas9/SpCas9 guides · source_derived_draft · unverified_draft

    The published human K562 dual-targeting screen reports SLC16A11/SLC25A39 genetic interaction pi=0.155012680942449 with significance value 0.0621187730457448 under Pyr15. This is a relative-fitness interaction estimate, not restoration of mitochondrial glutathione. primary_references: https://doi.org/10.1038/s41467-022-30126-9 primary_locator: Supplementary Data 3, Pyr15_piscore and Pyr15_pvalue, BD40 (row SLC25A39, column SLC16A11); Supplementary Data 4 All_Data_Sig_0.02 includes the glucose pair. organism: Human experimental_model: Human K562 pooled CRISPR screen; two batches; paired SaCas9/SpCas9 guides exposure: 15-day growth screen; 25 mM glucose, without added sodium pyruvate; 10% dialyzed FBS and 50 microgram/mL uridine. limitations: Positive pi describes departure from an additive fitness expectation, not positive regulation between wild-type proteins. All four pi estimates are below the authors absolute pi >0.25 large-effect cutoff. Glucose significance value is below 0.02; other conditions are not. These are authors reported BH-adjusted values according to their archived pi_score code, not new p-values. Guide constructs are not independent biological replicates. No new genotype-by-environment significance test was performed.
    Complete structured claim and evidence

  5. HEK293T cells expressing reference SLC16A11 showed approximately 45 percent higher pyruvate efflux rate than empty-vector controls in the reported assay.

    Reference SLC16A11 expression in human HEK293T cells → Human HEK293T pyruvate efflux assay source_derived_draftungraded
    Experimental context and source evidence
    experimental_model
    Human HEK293T cells expressing human reference SLC16A11-V5 and pyronic FRET sensor
    exposure
    Addition and withdrawal of 0.4 mM pyruvate; neutral pH; Figure 3E reports n=11 and P<0.05. Methods: 2 microgram pyronic and 2 microgram transporter/empty-vector plasmid, transfected 24 hours after plating on coverslips 72 hours before assay.
    limitations
    Selected primary main-text results, figure legend and methods reviewed through indexed PMC passages; supplements not independently reviewed. Difference was observed at neutral pH, not at acidic pH. This is not a K562 experiment and does not establish the mechanism of the genetic interaction. SLC16A11 has intracellular and cell-surface pools; pyruvate transport direction depends on gradients.
    organism
    Human
    primary_locator
    Results: SLC16A11 is a H+-coupled monocarboxylate transporter; Figure 3D-E; pyruvate transport methods.
    primary_references
    https://doi.org/10.1016/j.cell.2017.06.011

    SLC16A11 and mitochondrial glutathione: published screen connection and transport evidence · lines 51–57

    Primary sources 2022 and 2017, plus labelled descriptive reanalysis on 2026-09-20. · supports · Human HEK293T cells expressing human reference SLC16A11-V5 and pyronic FRET sensor · source_derived_draft · unverified_draft

    HEK293T cells expressing reference SLC16A11 showed approximately 45 percent higher pyruvate efflux rate than empty-vector controls in the reported assay. primary_references: https://doi.org/10.1016/j.cell.2017.06.011 primary_locator: Results: SLC16A11 is a H+-coupled monocarboxylate transporter; Figure 3D-E; pyruvate transport methods. organism: Human experimental_model: Human HEK293T cells expressing human reference SLC16A11-V5 and pyronic FRET sensor exposure: Addition and withdrawal of 0.4 mM pyruvate; neutral pH; Figure 3E reports n=11 and P<0.05. Methods: 2 microgram pyronic and 2 microgram transporter/empty-vector plasmid, transfected 24 hours after plating on coverslips 72 hours before assay. limitations: Selected primary main-text results, figure legend and methods reviewed through indexed PMC passages; supplements not independently reviewed. Difference was observed at neutral pH, not at acidic pH. This is not a K562 experiment and does not establish the mechanism of the genetic interaction. SLC16A11 has intracellular and cell-surface pools; pyruvate transport direction depends on gradients.
    Complete structured claim and evidence

  6. HEK293T cells expressing reference SLC16A11 showed approximately 45 percent higher pyruvate influx rate than empty-vector controls in the reported assay.

    Reference SLC16A11 expression in human HEK293T cells → Human HEK293T pyruvate influx assay source_derived_draftungraded
    Experimental context and source evidence
    experimental_model
    Human HEK293T cells expressing human reference SLC16A11-V5 and pyronic FRET sensor
    exposure
    Addition and withdrawal of 0.4 mM pyruvate; neutral pH; Figure 3E reports n=11 and P<0.05. Methods: 2 microgram pyronic and 2 microgram transporter/empty-vector plasmid, transfected 24 hours after plating on coverslips 72 hours before assay.
    limitations
    Selected primary main-text results, figure legend and methods reviewed through indexed PMC passages; supplements not independently reviewed. Difference was observed at neutral pH, not at acidic pH. This is not a K562 experiment and does not establish the mechanism of the genetic interaction. SLC16A11 has intracellular and cell-surface pools; pyruvate transport direction depends on gradients.
    organism
    Human
    primary_locator
    Results: SLC16A11 is a H+-coupled monocarboxylate transporter; Figure 3D-E; pyruvate transport methods.
    primary_references
    https://doi.org/10.1016/j.cell.2017.06.011

    SLC16A11 and mitochondrial glutathione: published screen connection and transport evidence · lines 42–48

    Primary sources 2022 and 2017, plus labelled descriptive reanalysis on 2026-09-20. · supports · Human HEK293T cells expressing human reference SLC16A11-V5 and pyronic FRET sensor · source_derived_draft · unverified_draft

    HEK293T cells expressing reference SLC16A11 showed approximately 45 percent higher pyruvate influx rate than empty-vector controls in the reported assay. primary_references: https://doi.org/10.1016/j.cell.2017.06.011 primary_locator: Results: SLC16A11 is a H+-coupled monocarboxylate transporter; Figure 3D-E; pyruvate transport methods. organism: Human experimental_model: Human HEK293T cells expressing human reference SLC16A11-V5 and pyronic FRET sensor exposure: Addition and withdrawal of 0.4 mM pyruvate; neutral pH; Figure 3E reports n=11 and P<0.05. Methods: 2 microgram pyronic and 2 microgram transporter/empty-vector plasmid, transfected 24 hours after plating on coverslips 72 hours before assay. limitations: Selected primary main-text results, figure legend and methods reviewed through indexed PMC passages; supplements not independently reviewed. Difference was observed at neutral pH, not at acidic pH. This is not a K562 experiment and does not establish the mechanism of the genetic interaction. SLC16A11 has intracellular and cell-surface pools; pyruvate transport direction depends on gradients.
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

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