{"id":"0bff8a41-6d81-5286-9e18-75245cd78d94","stable_key":"1c43c851-5763-5f8b-a7a0-000617c6fa2e:a11-a39-anti15","predicate":"has_contextual_experimental_result","statement":"The published human K562 dual-targeting screen reports SLC16A11/SLC25A39 genetic interaction pi=0.0805858670811976 with significance value 0.612290402312043 under Anti15. This is a relative-fitness interaction estimate, not restoration of mitochondrial glutathione.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"3e2fb0bc-bf9b-569a-910b-beca0151e7d2","mechanism_event_label":"a11-a39-anti15","subject":{"id":"ded03234-ba5e-587b-955d-e16a1be79907","slug":"human-slc16a11-slc25a39-double-knockout-k562","display_name":"Combined SLC16A11 and SLC25A39 targeting in human K562 screen","entity_type_key":"cellular_process"},"object":{"id":"c283c22f-162d-5795-9d4f-9d8d7c56ca38","slug":"human-k562-screen-relative-fitness","display_name":"Human K562 pooled screen relative fitness","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"3e2fb0bc-bf9b-569a-910b-beca0151e7d2","stable_key":"1c43c851-5763-5f8b-a7a0-000617c6fa2e:a11-a39-anti15","event_type":"experimental_result","label":"a11-a39-anti15","description":"The published human K562 dual-targeting screen reports SLC16A11/SLC25A39 genetic interaction pi=0.0805858670811976 with significance value 0.612290402312043 under Anti15. This is a relative-fitness interaction estimate, not restoration of mitochondrial glutathione.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ded03234-ba5e-587b-955d-e16a1be79907","slug":"human-slc16a11-slc25a39-double-knockout-k562","display_name":"Combined SLC16A11 and SLC25A39 targeting in human K562 screen","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c283c22f-162d-5795-9d4f-9d8d7c56ca38","slug":"human-k562-screen-relative-fitness","display_name":"Human K562 pooled screen relative fitness","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"4f426897-e417-5727-a32b-600ca909c89d","slug":"human-slc16a11","display_name":"Human monocarboxylate transporter SLC16A11","entity_type_key":"protein"},"role":"targeted gene","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"2b210ef5-c77e-57a3-be04-7876c67b8d75","slug":"slc25a39","display_name":"Human mitochondrial glutathione carrier SLC25A39","entity_type_key":"protein"},"role":"targeted gene","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"experimental_model","value_text":"Human K562 pooled CRISPR screen; two batches; paired SaCas9/SpCas9 guides","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"15-day growth screen; 25 mM glucose, 1 mM sodium pyruvate, 100 nM antimycin; 10% dialyzed FBS and 50 microgram/mL uridine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Positive pi describes departure from an additive fitness expectation, not positive regulation between wild-type proteins. All four pi estimates are below the authors absolute pi >0.25 large-effect cutoff. Glucose significance value is below 0.02; other conditions are not. These are authors reported BH-adjusted values according to their archived pi_score code, not new p-values. Guide constructs are not independent biological replicates. No new genotype-by-environment significance test was performed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Human","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"Supplementary Data 3, Anti15_piscore and Anti15_pvalue, BD40 (row SLC25A39, column SLC16A11); Supplementary Data 4 All_Data_Sig_0.02 includes the glucose pair.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1038/s41467-022-30126-9","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"7995acf2-a45b-5262-b9f3-f853df496cd7","evidence_kind":"source_excerpt","locator":"Lines 24-30","start_line":24,"end_line":30,"excerpt":"The published human K562 dual-targeting screen reports SLC16A11/SLC25A39 genetic interaction pi=0.0805858670811976 with significance value 0.612290402312043 under Anti15. This is a relative-fitness interaction estimate, not restoration of mitochondrial glutathione.\nprimary_references: https://doi.org/10.1038/s41467-022-30126-9\nprimary_locator: Supplementary Data 3, Anti15_piscore and Anti15_pvalue, BD40 (row SLC25A39, column SLC16A11); Supplementary Data 4 All_Data_Sig_0.02 includes the glucose pair.\norganism: Human\nexperimental_model: Human K562 pooled CRISPR screen; two batches; paired SaCas9/SpCas9 guides\nexposure: 15-day growth screen; 25 mM glucose, 1 mM sodium pyruvate, 100 nM antimycin; 10% dialyzed FBS and 50 microgram/mL uridine.\nlimitations: Positive pi describes departure from an additive fitness expectation, not positive regulation between wild-type proteins. All four pi estimates are below the authors absolute pi >0.25 large-effect cutoff. Glucose significance value is below 0.02; other conditions are not. These are authors reported BH-adjusted values according to their archived pi_score code, not new p-values. Guide constructs are not independent biological replicates. No new genotype-by-environment significance test was performed.","model_system":"Human K562 pooled CRISPR screen; two batches; paired SaCas9/SpCas9 guides","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Curator paraphrase plus exact spreadsheet coordinates; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d4f5e81d-ca2a-5aae-ae3c-c2b91b45534f","stable_key":"import-1c43c851-5763-5f8b-a7a0-000617c6fa2e","title":"SLC16A11 and mitochondrial glutathione: published screen connection and transport evidence","document_type":"imported_text","citation_label":"Primary sources 2022 and 2017, plus labelled descriptive reanalysis on 2026-09-20.","file_path":"","sha256":"bd8408dbf43c90d1fcf702f5c8ea704a7b13282329326eb09830d3ce3729bc91","revision_id":"6fc51201-9f8f-5177-826b-fd81470f6cb7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}