{"id":"0562b6c5-4814-5a56-a63f-d9022284c37e","stable_key":"1c43c851-5763-5f8b-a7a0-000617c6fa2e:a11-pyruvate-influx","predicate":"has_contextual_experimental_result","statement":"HEK293T cells expressing reference SLC16A11 showed approximately 45 percent higher pyruvate influx rate than empty-vector controls in the reported assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"09055ef9-70cc-5403-83c8-b9ec00c2df26","mechanism_event_label":"a11-pyruvate-influx","subject":{"id":"1a6a69bb-625f-573f-ad4a-501ef4c9b30a","slug":"human-slc16a11-expression-hek293t","display_name":"Reference SLC16A11 expression in human HEK293T cells","entity_type_key":"cellular_process"},"object":{"id":"c25ee7c0-fc29-5ceb-aa44-21ffd9b57d99","slug":"human-hek293t-pyruvate-influx","display_name":"Human HEK293T pyruvate influx assay","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"09055ef9-70cc-5403-83c8-b9ec00c2df26","stable_key":"1c43c851-5763-5f8b-a7a0-000617c6fa2e:a11-pyruvate-influx","event_type":"experimental_result","label":"a11-pyruvate-influx","description":"HEK293T cells expressing reference SLC16A11 showed approximately 45 percent higher pyruvate influx rate than empty-vector controls in the reported assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1a6a69bb-625f-573f-ad4a-501ef4c9b30a","slug":"human-slc16a11-expression-hek293t","display_name":"Reference SLC16A11 expression in human HEK293T cells","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c25ee7c0-fc29-5ceb-aa44-21ffd9b57d99","slug":"human-hek293t-pyruvate-influx","display_name":"Human HEK293T pyruvate influx assay","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"4f426897-e417-5727-a32b-600ca909c89d","slug":"human-slc16a11","display_name":"Human monocarboxylate transporter SLC16A11","entity_type_key":"protein"},"role":"expressed protein","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"91e6d4f5-fba1-542d-b68a-57cc28e1e425","slug":"pyruvate","display_name":"Pyruvate","entity_type_key":"small_molecule"},"role":"transported substrate","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"1ed0233a-3d10-58b3-abda-8ad04545c495","slug":"proton","display_name":"Proton","entity_type_key":"ion"},"role":"coupled ion","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Human HEK293T cells expressing human reference SLC16A11-V5 and pyronic FRET sensor","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Addition and withdrawal of 0.4 mM pyruvate; neutral pH; Figure 3E reports n=11 and P<0.05. Methods: 2 microgram pyronic and 2 microgram transporter/empty-vector plasmid, transfected 24 hours after plating on coverslips 72 hours before assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Selected primary main-text results, figure legend and methods reviewed through indexed PMC passages; supplements not independently reviewed. Difference was observed at neutral pH, not at acidic pH. This is not a K562 experiment and does not establish the mechanism of the genetic interaction. SLC16A11 has intracellular and cell-surface pools; pyruvate transport direction depends on gradients.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Human","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"Results: SLC16A11 is a H+-coupled monocarboxylate transporter; Figure 3D-E; pyruvate transport methods.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1016/j.cell.2017.06.011","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f5448abf-9127-59b9-a015-62c753615a86","evidence_kind":"source_excerpt","locator":"Lines 42-48","start_line":42,"end_line":48,"excerpt":"HEK293T cells expressing reference SLC16A11 showed approximately 45 percent higher pyruvate influx rate than empty-vector controls in the reported assay.\nprimary_references: https://doi.org/10.1016/j.cell.2017.06.011\nprimary_locator: Results: SLC16A11 is a H+-coupled monocarboxylate transporter; Figure 3D-E; pyruvate transport methods.\norganism: Human\nexperimental_model: Human HEK293T cells expressing human reference SLC16A11-V5 and pyronic FRET sensor\nexposure: Addition and withdrawal of 0.4 mM pyruvate; neutral pH; Figure 3E reports n=11 and P<0.05. Methods: 2 microgram pyronic and 2 microgram transporter/empty-vector plasmid, transfected 24 hours after plating on coverslips 72 hours before assay.\nlimitations: Selected primary main-text results, figure legend and methods reviewed through indexed PMC passages; supplements not independently reviewed. Difference was observed at neutral pH, not at acidic pH. This is not a K562 experiment and does not establish the mechanism of the genetic interaction. SLC16A11 has intracellular and cell-surface pools; pyruvate transport direction depends on gradients.","model_system":"Human HEK293T cells expressing human reference SLC16A11-V5 and pyronic FRET sensor","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Curator paraphrase plus exact spreadsheet coordinates; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d4f5e81d-ca2a-5aae-ae3c-c2b91b45534f","stable_key":"import-1c43c851-5763-5f8b-a7a0-000617c6fa2e","title":"SLC16A11 and mitochondrial glutathione: published screen connection and transport evidence","document_type":"imported_text","citation_label":"Primary sources 2022 and 2017, plus labelled descriptive reanalysis on 2026-09-20.","file_path":"","sha256":"bd8408dbf43c90d1fcf702f5c8ea704a7b13282329326eb09830d3ce3729bc91","revision_id":"6fc51201-9f8f-5177-826b-fd81470f6cb7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}