Component
Human ER-alpha-dependent reporter transcription in MCF-7 cells
Experimental model, exposure and limitations remain on each linked record.
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
Tartrazine activated the human ER-alpha reporter in MCF-7 cells, with a reported EC50 of 160 nM.
Experimental context and source evidence
- dose
- Tartrazine concentration response; reported EC50 160 nM
- duration
- Reporter exposure interval not specified in accessed abstract
- evidence_access
- Primary PubMed abstract; unrecovered method details explicitly retained.
- evidence_scope
- literature_reviewed; source-specific experimental curation
- experimental_model
- Human ESR1-positive MCF-7 breast cancer cells
- limitations
- Reporter activation is not direct receptor-binding proof or demonstrated estrogenic disease in humans; the proposed link to primary biliary disease remains a hypothesis.
- nutrient_topic
- Tartrazine food-colorant chapter; nutrient, drug and peptide interactions retain their models and limits. · Tartrazine
- organism
- Human ESR1-positive MCF-7 breast cancer cells
- plain_language
- Tartrazine activated the human ER-alpha reporter in MCF-7 cells, with a reported EC50 of 160 nM.
- primary_references
- Tartrazine and sunset yellow are xenoestrogens in a new screening assay to identify modulators of human oestrogen receptor transcriptional activity. (2012). https://pubmed.ncbi.nlm.nih.gov/22562034/ DOI: 10.1016/j.tox.2012.04.014
- route
- In vitro cell exposure
- tissue
- Estrogen-response-element reporter
Tartrazine: mechanisms, molecular forms and cross-actor connections (2026-09-20) · lines 270–279
Original AI-assisted curation of eighteen primary studies. Study-specific citations, negative findings and limitations retained. Not publisher full text. · supports · Human ESR1-positive MCF-7 breast cancer cells · source_derived_draft · unverified_draft
## tartrazine-estrogen-reporter Tartrazine activated the human ER-alpha reporter in MCF-7 cells, with a reported EC50 of 160 nM. Model/species: Human ESR1-positive MCF-7 breast cancer cells Tissue: Estrogen-response-element reporter Exposure: Tartrazine concentration response; reported EC50 160 nM Route: In vitro cell exposure Duration: Reporter exposure interval not specified in accessed abstract Limits: Reporter activation is not direct receptor-binding proof or demonstrated estrogenic disease in humans; the proposed link to primary biliary disease remains a hypothesis. Primary reference: Tartrazine and sunset yellow are xenoestrogens in a new screening assay to identify modulators of human oestrogen receptor transcriptional activity. (2012). https://pubmed.ncbi.nlm.nih.gov/22562034/ DOI: 10.1016/j.tox.2012.04.014 Access: Primary PubMed abstract; unrecovered method details explicitly retained.
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.