{"id":"853c4e0d-e5d9-5c0e-ae01-14632ecd9419","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-estrogen-reporter","predicate":"activates_reporter","statement":"Tartrazine activated the human ER-alpha reporter in MCF-7 cells, with a reported EC50 of 160 nM.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"a40e53c2-0344-5d07-a7d8-eb4e048eb8e7","mechanism_event_label":"Tartrazine activated the human ER-alpha reporter in MCF-7 cells, with a reported EC50 of 160 nM.","subject":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"object":{"id":"4e035117-2ba5-5797-8c58-ac9ce9264fe0","slug":"human-mcf7-esr1-reporter","display_name":"Human ER-alpha-dependent reporter transcription in MCF-7 cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a40e53c2-0344-5d07-a7d8-eb4e048eb8e7","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-estrogen-reporter-event","event_type":"observed_relationship","label":"Tartrazine activated the human ER-alpha reporter in MCF-7 cells, with a reported EC50 of 160 nM.","description":"Tartrazine activated the human ER-alpha reporter in MCF-7 cells, with a reported EC50 of 160 nM.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4e035117-2ba5-5797-8c58-ac9ce9264fe0","slug":"human-mcf7-esr1-reporter","display_name":"Human ER-alpha-dependent reporter transcription in MCF-7 cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b03c0784-d18d-58e3-99c5-2bcfed07028b","slug":"esr1","display_name":"Human estrogen receptor alpha / ESR1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Tartrazine concentration response; reported EC50 160 nM","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Reporter exposure interval not specified in accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract; unrecovered method details explicitly retained.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; source-specific experimental curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human ESR1-positive MCF-7 breast cancer cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Reporter activation is not direct receptor-binding proof or demonstrated estrogenic disease in humans; the proposed link to primary biliary disease remains a hypothesis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tartrazine food-colorant chapter; nutrient, drug and peptide interactions retain their models and limits.","comparator":null,"unit":null,"notes":"","entity":{"slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human ESR1-positive MCF-7 breast cancer cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Tartrazine activated the human ER-alpha reporter in MCF-7 cells, with a reported EC50 of 160 nM.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Tartrazine and sunset yellow are xenoestrogens in a new screening assay to identify modulators of human oestrogen receptor transcriptional activity. (2012). https://pubmed.ncbi.nlm.nih.gov/22562034/ DOI: 10.1016/j.tox.2012.04.014","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro cell exposure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Estrogen-response-element reporter","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6ce4d25d-f362-539c-84e4-0015f71a7644","evidence_kind":"source_excerpt","locator":"Lines 270-279","start_line":270,"end_line":279,"excerpt":"## tartrazine-estrogen-reporter\nTartrazine activated the human ER-alpha reporter in MCF-7 cells, with a reported EC50 of 160 nM.\nModel/species: Human ESR1-positive MCF-7 breast cancer cells\nTissue: Estrogen-response-element reporter\nExposure: Tartrazine concentration response; reported EC50 160 nM\nRoute: In vitro cell exposure\nDuration: Reporter exposure interval not specified in accessed abstract\nLimits: Reporter activation is not direct receptor-binding proof or demonstrated estrogenic disease in humans; the proposed link to primary biliary disease remains a hypothesis.\nPrimary reference: Tartrazine and sunset yellow are xenoestrogens in a new screening assay to identify modulators of human oestrogen receptor transcriptional activity. (2012). https://pubmed.ncbi.nlm.nih.gov/22562034/ DOI: 10.1016/j.tox.2012.04.014\nAccess: Primary PubMed abstract; unrecovered method details explicitly retained.","model_system":"Human ESR1-positive MCF-7 breast cancer cells","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span, not a publisher quotation; primary citation retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d9540941-68b4-5a0d-a5c1-828665a918e2","stable_key":"import-792249b7-a38d-5f43-afb6-2936818a3fa2","title":"Tartrazine: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of eighteen primary studies. Study-specific citations, negative findings and limitations retained. 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