Component
LY294002 treatment of human osteoblast-like cells
Context-specific entity; species, compartment and exposure are stated on each claim.
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What it acts on
LY294002 reduced pathway signals and osteogenic markers during orthosilicic acid treatment.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- evidence_access
- Primary abstract
- experimental_model
- Human osteoblast-like cultures.
- limitations
- LY294002 has off-target effects and is not an inhibitor specific to the entire PI3K–Akt–mTOR chain.
- nutrient_topic
- Silica collection; molecular form, preparation, species, exposure and manipulation remain explicit. · Silica and soluble silicon
- plain_language
- The cell response was sensitive to PI3K-pathway blockade.
- primary_references
- Orthosilicic Acid Accelerates Bone Formation in Human Osteoblast-Like Cells Through the PI3K-Akt-mTOR Pathway. · 2019 · https://pubmed.ncbi.nlm.nih.gov/30421162/ · DOI 10.1007/s12011-018-1574-9
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Silica: soluble silicon, cellular transport and particle-specific mechanisms (2026-09-19) · lines 176–182
AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text. · supports · Human osteoblast-like cultures. · source_derived_draft · unverified_draft
## silica-ly294002-block The cell response was sensitive to PI3K-pathway blockade. LY294002 reduced pathway signals and osteogenic markers during orthosilicic acid treatment. Model: Human osteoblast-like cultures. Limitations: LY294002 has off-target effects and is not an inhibitor specific to the entire PI3K–Akt–mTOR chain. Evidence access: Primary abstract Orthosilicic Acid Accelerates Bone Formation in Human Osteoblast-Like Cells Through the PI3K-Akt-mTOR Pathway. · 2019 · https://pubmed.ncbi.nlm.nih.gov/30421162/ · DOI 10.1007/s12011-018-1574-9
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.