{"id":"ec6e6661-45eb-55fd-acdf-35cbb9043d84","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-ly294002-block","predicate":"reduces","statement":"LY294002 reduced pathway signals and osteogenic markers during orthosilicic acid treatment.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"8b4f3d85-d82c-510b-a786-24450bc48573","mechanism_event_label":"The cell response was sensitive to PI3K-pathway blockade.","subject":{"id":"8a487863-4030-5ebf-893c-85d627beba48","slug":"human-ly294002-osteoblast-treatment","display_name":"LY294002 treatment of human osteoblast-like cells","entity_type_key":"cellular_process"},"object":{"id":"8a31fdbb-3012-50f8-8be2-1c5b373aa5c7","slug":"human-osa-collagen-response","display_name":"Collagen synthesis response to orthosilicic acid","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"8b4f3d85-d82c-510b-a786-24450bc48573","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-ly294002-block-event","event_type":"observed_relationship","label":"The cell response was sensitive to PI3K-pathway blockade.","description":"LY294002 reduced pathway signals and osteogenic markers during orthosilicic acid treatment.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8a487863-4030-5ebf-893c-85d627beba48","slug":"human-ly294002-osteoblast-treatment","display_name":"LY294002 treatment of human osteoblast-like cells","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8a31fdbb-3012-50f8-8be2-1c5b373aa5c7","slug":"human-osa-collagen-response","display_name":"Collagen synthesis response to orthosilicic acid","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"11b1cdb5-1b10-586b-9917-b4da18ac072f","slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"c497144d-1e90-5176-a475-2d715992f5b0","slug":"orthosilicic-acid","display_name":"Orthosilicic acid / Si(OH)4","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"779b2149-a16f-58b8-815b-7f1f2620da1f","slug":"mtor","display_name":"Human mechanistic target of rapamycin kinase / MTOR","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"a13cb592-c939-5f92-9206-d774248802fc","slug":"akt-proteins","display_name":"AKT serine/threonine kinase family","entity_type_key":"protein_family"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human osteoblast-like cultures.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"LY294002 has off-target effects and is not an inhibitor specific to the entire PI3K–Akt–mTOR chain.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Silica collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"The cell response was sensitive to PI3K-pathway blockade.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Orthosilicic Acid Accelerates Bone Formation in Human Osteoblast-Like Cells Through the PI3K-Akt-mTOR Pathway. · 2019 · https://pubmed.ncbi.nlm.nih.gov/30421162/ · DOI 10.1007/s12011-018-1574-9","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"6475082f-67be-5461-a4ef-d7f391a8d1e8","evidence_kind":"source_excerpt","locator":"Lines 176-182","start_line":176,"end_line":182,"excerpt":"## silica-ly294002-block\nThe cell response was sensitive to PI3K-pathway blockade.\nLY294002 reduced pathway signals and osteogenic markers during orthosilicic acid treatment.\nModel: Human osteoblast-like cultures.\nLimitations: LY294002 has off-target effects and is not an inhibitor specific to the entire PI3K–Akt–mTOR chain.\nEvidence access: Primary abstract\nOrthosilicic Acid Accelerates Bone Formation in Human Osteoblast-Like Cells Through the PI3K-Akt-mTOR Pathway. · 2019 · https://pubmed.ncbi.nlm.nih.gov/30421162/ · DOI 10.1007/s12011-018-1574-9","model_system":"Human osteoblast-like cultures.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc4b461e-dd46-57ab-8213-8aeb7ff9e8d0","stable_key":"import-3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd","title":"Silica: soluble silicon, cellular transport and particle-specific mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"e05d81962a1ca6cbfc68fce4fd3dbcdea67c8e61332c19f4fec5d0c3ab8bf1b9","revision_id":"6433ed49-a331-571b-a611-21e56c3ce4bc","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}