Component
Cultured human hepatocyte viability, preparation specified
Cultured human hepatocyte viability, preparation specified. Species, exposure and limitations are retained in each linked claim.
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
The study reported sulforaphane cytotoxicity IC50 values of 25.1 micromolar in hepatocytes and 56.4 micromolar in HepG2 cells alongside lower-exposure protective experiments.
Experimental context and source evidence
- evidence_span
- {"source_cache": "artifacts/sulforaphane-research/25683399.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "bfaef8428737a984f51d1c91e9f6dddaf1d40a19c8ced9ddd7f6a222a65e450e", "start_char": 0, "end_char": 1707, "text_sha256": "bfaef8428737a984f51d1c91e9f6dddaf1d40a19c8ced9ddd7f6a222a65e450e"}
- experimental_model
- Enzyme induction, viability and knockdown experiments
- exposure
- Sulforaphane with or without selenium; peroxide challenge
- limitations
- Model-specific cytotoxicity; no conversion of culture concentrations into a safe or toxic oral dose.
- nutrient_topic
- Sulforaphane research collection; topical membership is not evidence of a direct dietary effect. · Sulforaphane / SFN, stereochemistry specified per study
- organism
- Immortalized human hepatocytes and HepG2 comparison
- plain_language
- Exposure and cell type can change whether the effect is protective or harmful.
- primary_references
- [sulforaphane-p25683399] Synergy between sulforaphane and selenium in the up-regulation of thioredoxin reductase and protection against hydrogen peroxide-induced cell death in human hepatocytes. (2012). https://pubmed.ncbi.nlm.nih.gov/25683399/ DOI: 10.1016/j.foodchem.2012.01.026
- tissue_or_cell_type
- TXNRD1 response and toxicity
Sulforaphane: formation, electrophile sensing and nutrient connections (2026-09-17) · lines 801–812
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Enzyme induction, viability and knockdown experiments · source_derived_draft · unverified_draft
### sulforaphane-hepatocyte-toxicity The study reported sulforaphane cytotoxicity IC50 values of 25.1 micromolar in hepatocytes and 56.4 micromolar in HepG2 cells alongside lower-exposure protective experiments. Condition category: normal nutrient_topic: Sulforaphane research collection; topical membership is not evidence of a direct dietary effect. plain_language: Exposure and cell type can change whether the effect is protective or harmful. organism: Immortalized human hepatocytes and HepG2 comparison tissue_or_cell_type: TXNRD1 response and toxicity experimental_model: Enzyme induction, viability and knockdown experiments limitations: Model-specific cytotoxicity; no conversion of culture concentrations into a safe or toxic oral dose. exposure: Sulforaphane with or without selenium; peroxide challenge evidence_span: {"source_cache": "artifacts/sulforaphane-research/25683399.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "bfaef8428737a984f51d1c91e9f6dddaf1d40a19c8ced9ddd7f6a222a65e450e", "start_char": 0, "end_char": 1707, "text_sha256": "bfaef8428737a984f51d1c91e9f6dddaf1d40a19c8ced9ddd7f6a222a65e450e"} [sulforaphane-p25683399] Synergy between sulforaphane and selenium in the up-regulation of thioredoxin reductase and protection against hydrogen peroxide-induced cell death in human hepatocytes. (2012). https://pubmed.ncbi.nlm.nih.gov/25683399/ DOI: 10.1016/j.foodchem.2012.01.026
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.