{"id":"dc2526fe-780b-5325-99ee-bfa6ca04883b","stable_key":"44eaeae5-557a-552b-8998-884f30462e2a:sulforaphane-hepatocyte-toxicity","predicate":"showed_cell_dependent_effects_on","statement":"The study reported sulforaphane cytotoxicity IC50 values of 25.1 micromolar in hepatocytes and 56.4 micromolar in HepG2 cells alongside lower-exposure protective experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"25e0f61d-d6b7-578f-bf21-b803d59d3531","mechanism_event_label":"Exposure and cell type can change whether the effect is protective or harmful.","subject":{"id":"67e70215-70c4-546a-84b4-0819fac0b326","slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"},"object":{"id":"58bf7a96-2f5d-541d-a869-5bc6b7ef7cf0","slug":"human-hepatocyte-viability","display_name":"Cultured human hepatocyte viability, preparation specified","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"25e0f61d-d6b7-578f-bf21-b803d59d3531","stable_key":"44eaeae5-557a-552b-8998-884f30462e2a:sulforaphane-hepatocyte-toxicity-event","event_type":"biochemical_relationship","label":"Exposure and cell type can change whether the effect is protective or harmful.","description":"The study reported sulforaphane cytotoxicity IC50 values of 25.1 micromolar in hepatocytes and 56.4 micromolar in HepG2 cells alongside lower-exposure protective experiments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"67e70215-70c4-546a-84b4-0819fac0b326","slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"58bf7a96-2f5d-541d-a869-5bc6b7ef7cf0","slug":"human-hepatocyte-viability","display_name":"Cultured human hepatocyte viability, preparation specified","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/sulforaphane-research/25683399.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"bfaef8428737a984f51d1c91e9f6dddaf1d40a19c8ced9ddd7f6a222a65e450e\", \"start_char\": 0, \"end_char\": 1707, \"text_sha256\": \"bfaef8428737a984f51d1c91e9f6dddaf1d40a19c8ced9ddd7f6a222a65e450e\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Enzyme induction, viability and knockdown experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Sulforaphane with or without selenium; peroxide challenge","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Model-specific cytotoxicity; no conversion of culture concentrations into a safe or toxic oral dose.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Sulforaphane research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Immortalized human hepatocytes and HepG2 comparison","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Exposure and cell type can change whether the effect is protective or harmful.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[sulforaphane-p25683399] Synergy between sulforaphane and selenium in the up-regulation of thioredoxin reductase and protection against hydrogen peroxide-induced cell death in human hepatocytes. (2012). https://pubmed.ncbi.nlm.nih.gov/25683399/ DOI: 10.1016/j.foodchem.2012.01.026","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"TXNRD1 response and toxicity","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"fb605af2-e848-54e1-87f5-815057593f9b","evidence_kind":"source_excerpt","locator":"Lines 801-812","start_line":801,"end_line":812,"excerpt":"### sulforaphane-hepatocyte-toxicity\nThe study reported sulforaphane cytotoxicity IC50 values of 25.1 micromolar in hepatocytes and 56.4 micromolar in HepG2 cells alongside lower-exposure protective experiments.\nCondition category: normal\nnutrient_topic: Sulforaphane research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Exposure and cell type can change whether the effect is protective or harmful.\norganism: Immortalized human hepatocytes and HepG2 comparison\ntissue_or_cell_type: TXNRD1 response and toxicity\nexperimental_model: Enzyme induction, viability and knockdown experiments\nlimitations: Model-specific cytotoxicity; no conversion of culture concentrations into a safe or toxic oral dose.\nexposure: Sulforaphane with or without selenium; peroxide challenge\nevidence_span: {\"source_cache\": \"artifacts/sulforaphane-research/25683399.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"bfaef8428737a984f51d1c91e9f6dddaf1d40a19c8ced9ddd7f6a222a65e450e\", \"start_char\": 0, \"end_char\": 1707, \"text_sha256\": \"bfaef8428737a984f51d1c91e9f6dddaf1d40a19c8ced9ddd7f6a222a65e450e\"}\n[sulforaphane-p25683399] Synergy between sulforaphane and selenium in the up-regulation of thioredoxin reductase and protection against hydrogen peroxide-induced cell death in human hepatocytes. (2012). https://pubmed.ncbi.nlm.nih.gov/25683399/ DOI: 10.1016/j.foodchem.2012.01.026","model_system":"Enzyme induction, viability and knockdown experiments","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [sulforaphane-p25683399] Synergy between sulforaphane and selenium in the up-regulation of thioredoxin reductase and protection against hydrogen peroxide-induced cell death in human hepatocytes. 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