Component
HRK1 inward-rectifier potassium channel in the cloned-channel assay
Context-specific entity; species, compartment and exposure are stated on each claim.
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
Cytoplasmic spermidine caused reversible voltage-dependent rectification of cloned HRK1 channels.
Experimental context and source evidence
- evidence_access
- Primary abstract
- experimental_model
- Xenopus oocyte inside-out patches; construct species unresolved in accessed abstract.
- limitations
- Do not relabel the channel as a verified human isoform or infer a serum-potassium effect.
- nutrient_topic
- Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit. · Spermidine
- plain_language
- Spermidine can limit outward potassium current.
- primary_references
- The mechanism of inward rectification of potassium channels: "long-pore plugging" by cytoplasmic polyamines. · 1995 · https://pubmed.ncbi.nlm.nih.gov/8648298/ · DOI 10.1085/jgp.106.5.923
Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19) · lines 430–436
AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text. · supports · Xenopus oocyte inside-out patches; construct species unresolved in accessed abstract. · source_derived_draft · unverified_draft
## spermidine-kir-block Spermidine can limit outward potassium current. Cytoplasmic spermidine caused reversible voltage-dependent rectification of cloned HRK1 channels. Model: Xenopus oocyte inside-out patches; construct species unresolved in accessed abstract. Limitations: Do not relabel the channel as a verified human isoform or infer a serum-potassium effect. Evidence access: Primary abstract The mechanism of inward rectification of potassium channels: "long-pore plugging" by cytoplasmic polyamines. · 1995 · https://pubmed.ncbi.nlm.nih.gov/8648298/ · DOI 10.1085/jgp.106.5.923
Complete structured claim and evidence
Where it participates (unsigned role)
Magnesium-associated unblock was almost instantaneous, whereas spermidine unblock was time dependent.
Experimental context and source evidence
- evidence_access
- Primary abstract
- experimental_model
- HRK1 patch-clamp comparison.
- limitations
- This is a gating comparison, not evidence that one nutrient replaces the other.
- nutrient_topic
- Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit. · Spermidine
- plain_language
- Magnesium and spermidine influence the same channel with different kinetics.
- primary_references
- The mechanism of inward rectification of potassium channels: "long-pore plugging" by cytoplasmic polyamines. · 1995 · https://pubmed.ncbi.nlm.nih.gov/8648298/ · DOI 10.1085/jgp.106.5.923
Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19) · lines 438–444
AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text. · supports · HRK1 patch-clamp comparison. · source_derived_draft · unverified_draft
## spermidine-kir-magnesium Magnesium and spermidine influence the same channel with different kinetics. Magnesium-associated unblock was almost instantaneous, whereas spermidine unblock was time dependent. Model: HRK1 patch-clamp comparison. Limitations: This is a gating comparison, not evidence that one nutrient replaces the other. Evidence access: Primary abstract The mechanism of inward rectification of potassium channels: "long-pore plugging" by cytoplasmic polyamines. · 1995 · https://pubmed.ncbi.nlm.nih.gov/8648298/ · DOI 10.1085/jgp.106.5.923
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.