{"id":"5e997992-bc40-5234-9f94-6ece67c976fc","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-kir-magnesium","predicate":"differs_in_unblocking_from","statement":"Magnesium-associated unblock was almost instantaneous, whereas spermidine unblock was time dependent.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c127b1d4-8aea-5502-a6b3-ef90812f8ef6","mechanism_event_label":"Magnesium and spermidine influence the same channel with different kinetics.","subject":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"object":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"c127b1d4-8aea-5502-a6b3-ef90812f8ef6","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-kir-magnesium-event","event_type":"observed_relationship","label":"Magnesium and spermidine influence the same channel with different kinetics.","description":"Magnesium-associated unblock was almost instantaneous, whereas spermidine unblock was time dependent.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"23f993e0-3348-5205-b71f-1bb38b4fa4ff","slug":"hrk1-channel-assay","display_name":"HRK1 inward-rectifier potassium channel in the cloned-channel assay","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"5dd31e52-f51e-51f3-880e-240abcc0ab1d","slug":"potassium-ion","display_name":"Potassium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"HRK1 patch-clamp comparison.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This is a gating comparison, not evidence that one nutrient replaces the other.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Magnesium and spermidine influence the same channel with different kinetics.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The mechanism of inward rectification of potassium channels: \"long-pore plugging\" by cytoplasmic polyamines. · 1995 · https://pubmed.ncbi.nlm.nih.gov/8648298/ · DOI 10.1085/jgp.106.5.923","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e9ab45e9-13a3-5d22-9400-df802338b6f2","evidence_kind":"source_excerpt","locator":"Lines 438-444","start_line":438,"end_line":444,"excerpt":"## spermidine-kir-magnesium\nMagnesium and spermidine influence the same channel with different kinetics.\nMagnesium-associated unblock was almost instantaneous, whereas spermidine unblock was time dependent.\nModel: HRK1 patch-clamp comparison.\nLimitations: This is a gating comparison, not evidence that one nutrient replaces the other.\nEvidence access: Primary abstract\nThe mechanism of inward rectification of potassium channels: \"long-pore plugging\" by cytoplasmic polyamines. · 1995 · https://pubmed.ncbi.nlm.nih.gov/8648298/ · DOI 10.1085/jgp.106.5.923","model_system":"HRK1 patch-clamp comparison.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4348f3f8-0180-58a3-b61c-55d03bb322d2","stable_key":"import-88eb7407-f147-558b-889b-0c89c4e0aa4a","title":"Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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