Component
HADHA TRIPLE-350-383-406 experimental construct
Study-specific entity. Experimental conditions and evidence limits remain on each linked claim.
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What it acts on
The HADHA 350/383/406 triple mutant remained susceptible to Dlat-induced acetylation in HEK293 cells.
Experimental context and source evidence
- access_level
- full_text_and_supplement_review
- compartment
- Immunoprecipitated tagged protein
- dose
- Construct amounts not specified in reviewed text
- duration
- Not specified in reviewed text
- endpoint
- hadha-construct-lysine-acetylation
- evidence_location
- Figure 7F
- experimental_model
- Flag-HADHA point-mutant/combined-mutant transfection
- exposure
- hadha-triple-350-383-406-construct
- limitations
- Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. The negative control is limited to the tested substitutions; it does not exclude all other cooperating residues.
- organism
- Homo sapiens HEK293 host; construct sequence species not assumed from host
- plain_language
- The HADHA 350/383/406 triple mutant remained susceptible to Dlat-induced acetylation in HEK293 cells.
- primary_locator
- [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 48, "text_sha256": "134dcda50e7eafd608062a092c3cab71e5febe5935586f7d7d478988d6a3ea8e", "xml_element_id": "Par20"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 50, "text_sha256": "ef3c37a398cc33c2cac08d4fc06ef96648c5fcda5ce3de26387b1afb609af2bf", "xml_element_id": null}]
- primary_references
- https://doi.org/10.1038/s41467-026-70703-w
- sample_size
- 4 per group
- tissue_or_cell_type
- HEK293 cells
DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence · lines 124–139
AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation. · supports · Flag-HADHA point-mutant/combined-mutant transfection · source_derived_draft · unverified_draft
The HADHA 350/383/406 triple mutant remained susceptible to Dlat-induced acetylation in HEK293 cells. organism: Homo sapiens HEK293 host; construct sequence species not assumed from host tissue_or_cell_type: HEK293 cells experimental_model: Flag-HADHA point-mutant/combined-mutant transfection compartment: Immunoprecipitated tagged protein dose: Construct amounts not specified in reviewed text duration: Not specified in reviewed text primary_references: https://doi.org/10.1038/s41467-026-70703-w access_level: full_text_and_supplement_review evidence_location: Figure 7F endpoint: hadha-construct-lysine-acetylation exposure: hadha-triple-350-383-406-construct limitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. The negative control is limited to the tested substitutions; it does not exclude all other cooperating residues. primary_locator: [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 48, "text_sha256": "134dcda50e7eafd608062a092c3cab71e5febe5935586f7d7d478988d6a3ea8e", "xml_element_id": "Par20"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 50, "text_sha256": "ef3c37a398cc33c2cac08d4fc06ef96648c5fcda5ce3de26387b1afb609af2bf", "xml_element_id": null}] plain_language: The HADHA 350/383/406 triple mutant remained susceptible to Dlat-induced acetylation in HEK293 cells. sample_size: 4 per group
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.