{"id":"8bf08d06-5a55-5f01-a7c8-d935b83c3ea0","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:triple-mutant-not-protective","predicate":"retains_induced_response","statement":"The HADHA 350/383/406 triple mutant remained susceptible to Dlat-induced acetylation in HEK293 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"83845e4b-71a9-53ee-ba13-2d1fe24a3dbb","mechanism_event_label":"triple mutant not protective","subject":{"id":"f595294a-6cf1-52ed-9232-5793f33f08d4","slug":"hadha-triple-350-383-406-construct","display_name":"HADHA TRIPLE-350-383-406 experimental construct","entity_type_key":"protein_state"},"object":{"id":"f47c958c-2a43-5abc-8842-31700b1efa4c","slug":"hadha-construct-lysine-acetylation","display_name":"Acetyl-lysine signal on experimental HADHA constructs","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"83845e4b-71a9-53ee-ba13-2d1fe24a3dbb","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:triple-mutant-not-protective","event_type":"retains_induced_response","label":"triple mutant not protective","description":"Model-specific finding; read linked claim context and source-access tier.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f595294a-6cf1-52ed-9232-5793f33f08d4","slug":"hadha-triple-350-383-406-construct","display_name":"HADHA TRIPLE-350-383-406 experimental construct","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f47c958c-2a43-5abc-8842-31700b1efa4c","slug":"hadha-construct-lysine-acetylation","display_name":"Acetyl-lysine signal on experimental HADHA constructs","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"access_level","value_text":"full_text_and_supplement_review","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"compartment","value_text":"Immunoprecipitated tagged protein","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"Construct amounts not specified in reviewed text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Not specified in reviewed text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"endpoint","value_text":"hadha-construct-lysine-acetylation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Figure 7F","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Flag-HADHA point-mutant/combined-mutant transfection","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"hadha-triple-350-383-406-construct","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. The negative control is limited to the tested substitutions; it does not exclude all other cooperating residues.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Homo sapiens HEK293 host; construct sequence species not assumed from host","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The HADHA 350/383/406 triple mutant remained susceptible to Dlat-induced acetylation in HEK293 cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"[{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 48, \"text_sha256\": \"134dcda50e7eafd608062a092c3cab71e5febe5935586f7d7d478988d6a3ea8e\", \"xml_element_id\": \"Par20\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 50, \"text_sha256\": \"ef3c37a398cc33c2cac08d4fc06ef96648c5fcda5ce3de26387b1afb609af2bf\", \"xml_element_id\": null}]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1038/s41467-026-70703-w","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"sample_size","value_text":"4 per group","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HEK293 cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0c571a36-3200-55f7-afe6-595f7ee3a275","evidence_kind":"source_excerpt","locator":"Lines 124-139","start_line":124,"end_line":139,"excerpt":"The HADHA 350/383/406 triple mutant remained susceptible to Dlat-induced acetylation in HEK293 cells.\norganism: Homo sapiens HEK293 host; construct sequence species not assumed from host\ntissue_or_cell_type: HEK293 cells\nexperimental_model: Flag-HADHA point-mutant/combined-mutant transfection\ncompartment: Immunoprecipitated tagged protein\ndose: Construct amounts not specified in reviewed text\nduration: Not specified in reviewed text\nprimary_references: https://doi.org/10.1038/s41467-026-70703-w\naccess_level: full_text_and_supplement_review\nevidence_location: Figure 7F\nendpoint: hadha-construct-lysine-acetylation\nexposure: hadha-triple-350-383-406-construct\nlimitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. The negative control is limited to the tested substitutions; it does not exclude all other cooperating residues.\nprimary_locator: [{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 48, \"text_sha256\": \"134dcda50e7eafd608062a092c3cab71e5febe5935586f7d7d478988d6a3ea8e\", \"xml_element_id\": \"Par20\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 50, \"text_sha256\": \"ef3c37a398cc33c2cac08d4fc06ef96648c5fcda5ce3de26387b1afb609af2bf\", \"xml_element_id\": null}]\nplain_language: The HADHA 350/383/406 triple mutant remained susceptible to Dlat-induced acetylation in HEK293 cells.\nsample_size: 4 per group","model_system":"Flag-HADHA point-mutant/combined-mutant transfection","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Quotation is the authored curation, not original publisher wording. Exact primary locators and access limits retained in contexts.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"ee72956d-4fd4-58d3-9a94-e173bb1e3282","stable_key":"import-c156003d-8cdf-5259-8561-9803795ecc82","title":"DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence","document_type":"imported_text","citation_label":"AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation.","file_path":"","sha256":"159e5472c8b1d104625f9c648dc9ee24dcd5fe102109fd960bba50e9ba9de954","revision_id":"5ca6b1f1-fd75-5f8d-ba2b-0215fbf387c7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}