Component
Glycolytic enzyme abundance
Proteomic readout of glycolysis-related enzyme abundance; not a direct flux measurement.
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
Eight glycolytic enzymes increased in abundance after three days of riboflavin starvation in B16 melanoma cells.
Experimental context and source evidence
- availability_state
- nutrient_deficiency Imported condition classification; unverified.
- evidence_spans
- [{"source_bundle": "artifacts/riboflavin_metabolism_sources.json", "source_key": "PMC7477094", "locator": "XML .//body//p", "paragraph_index": 21, "char_start": 0, "char_end": 3026, "evidence_access": "full-text"}]
- experimental_model
- Mouse B16 melanoma cells cultured in riboflavin-free medium for 3 days; quantitative proteomics.
- exposure
- Three-day riboflavin withdrawal; mass-spectrometry proteomics.
- limitations
- The response does not demonstrate complete energetic compensation or measured glycolytic flux.
- nutrient_topic
- Riboflavin research collection; topical membership is not evidence of a direct dietary effect. · Riboflavin (vitamin B2)
- organism
- Mus musculus
- plain_language
- These cells increased glycolytic machinery while their respiratory machinery declined.
- primary_references
- [martinez-limon-2020-b2-depletion] Flavin dependency undermines proteome stability, lipid metabolism and cellular proliferation during vitamin B2 deficiency (2020). https://pubmed.ncbi.nlm.nih.gov/32895367/ DOI: 10.1038/s41419-020-02929-5
- tissue_or_cell_type
- B16 melanoma cells
- trigger_kind
- nutrient_deficiency Imported condition classification; unverified.
Riboflavin: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 983–994
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Mouse B16 melanoma cells cultured in riboflavin-free medium for 3 days; quantitative proteomics. · source_derived_draft · unverified_draft
### b2-met-b16-glycolytic-proteome Eight glycolytic enzymes increased in abundance after three days of riboflavin starvation in B16 melanoma cells. Condition category: nutrient_deficiency nutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect. plain_language: These cells increased glycolytic machinery while their respiratory machinery declined. organism: Mus musculus tissue_or_cell_type: B16 melanoma cells experimental_model: Mouse B16 melanoma cells cultured in riboflavin-free medium for 3 days; quantitative proteomics. limitations: The response does not demonstrate complete energetic compensation or measured glycolytic flux. exposure: Three-day riboflavin withdrawal; mass-spectrometry proteomics. evidence_spans: [{"source_bundle": "artifacts/riboflavin_metabolism_sources.json", "source_key": "PMC7477094", "locator": "XML .//body//p", "paragraph_index": 21, "char_start": 0, "char_end": 3026, "evidence_access": "full-text"}] [martinez-limon-2020-b2-depletion] Flavin dependency undermines proteome stability, lipid metabolism and cellular proliferation during vitamin B2 deficiency (2020). https://pubmed.ncbi.nlm.nih.gov/32895367/ DOI: 10.1038/s41419-020-02929-5
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.