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unverified.","entity":null}],"evidence":[{"id":"adf0dbd0-6196-535b-9e34-eb1075ac8511","evidence_kind":"source_excerpt","locator":"Lines 983-994","start_line":983,"end_line":994,"excerpt":"### b2-met-b16-glycolytic-proteome\nEight glycolytic enzymes increased in abundance after three days of riboflavin starvation in B16 melanoma cells.\nCondition category: nutrient_deficiency\nnutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: These cells increased glycolytic machinery while their respiratory machinery declined.\norganism: Mus musculus\ntissue_or_cell_type: B16 melanoma cells\nexperimental_model: Mouse B16 melanoma cells cultured in riboflavin-free medium for 3 days; quantitative proteomics.\nlimitations: The response does not demonstrate complete energetic compensation or measured glycolytic flux.\nexposure: Three-day riboflavin withdrawal; mass-spectrometry proteomics.\nevidence_spans: [{\"source_bundle\": \"artifacts/riboflavin_metabolism_sources.json\", \"source_key\": \"PMC7477094\", \"locator\": \"XML .//body//p\", \"paragraph_index\": 21, \"char_start\": 0, \"char_end\": 3026, \"evidence_access\": \"full-text\"}]\n[martinez-limon-2020-b2-depletion] Flavin dependency undermines proteome stability, lipid metabolism and cellular proliferation during vitamin B2 deficiency (2020). https://pubmed.ncbi.nlm.nih.gov/32895367/ DOI: 10.1038/s41419-020-02929-5","model_system":"Mouse B16 melanoma cells cultured in riboflavin-free medium for 3 days; 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Study references: [martinez-limon-2020-b2-depletion] Flavin dependency undermines proteome stability, lipid metabolism and cellular proliferation during vitamin B2 deficiency (2020). https://pubmed.ncbi.nlm.nih.gov/32895367/ DOI: 10.1038/s41419-020-02929-5","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f7c9578-82bf-5e2d-b5c4-72a79fb4f6af","stable_key":"import-548ab9d6-3a9b-5bed-879c-17d03813b636","title":"Riboflavin: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"680cb6bc8249877f2410f551807f10d390fdd719014137d7414ba3420e29228d","revision_id":"7a61e299-908d-5372-860b-99ed190f9d7a","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}