Component

The GABA-A receptor subunit repertoire of a cell

The GABA-A receptor subunit repertoire of a cell. Species, exposure and limitations are retained in each linked claim.

2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What it acts on

  1. There were 5, 8 and 13 different GABA-A subunit isoforms identified in human, mouse and rat CD4-positive and CD8-positive T cells respectively, importantly the gamma2 subunit that imposes benzodiazepine sensitivity on the GABA-A receptors was only detected in the mouse T cells, immunoblots and immunocytochemistry showed abundant GABA-A channel proteins in T cells from all three species, GABA-activated whole-cell transient and tonic currents were recorded and were inhibited by picrotoxin, SR95531 and bicuculline, and it is important to bear in mind the interspecies difference when selecting the appropriate animal models and when selecting drugs aimed at modulating human T cell function.

    The GABA-A receptor subunit repertoire of a cell → GABA-A receptor anion channels source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/gaba-research/22927941.abstract.txt", "locator": "Indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "0457001832d2a3595899415221b7797f5288a787c1d52fd6afa1acf24f032402", "start_char": 0, "end_char": 1589, "text_sha256": "0457001832d2a3595899415221b7797f5288a787c1d52fd6afa1acf24f032402"}
    experimental_model
    Subunit isoform survey with immunoblotting, immunocytochemistry and whole-cell recording across three species
    exposure
    GABA-activated transient and tonic currents, with picrotoxin, SR95531 and bicuculline
    limitations
    The species comparison is the point. Subunit repertoires differ enough that a drug result in one species does not carry to another.
    nutrient_topic
    GABA research collection; topical membership is not evidence of a direct clinical effect, and the sign of a GABA response depends on the chloride gradient of the cell it was measured in. · Gamma-aminobutyric acid
    organism
    Human, mouse and rat
    plain_language
    The subunit that makes a receptor respond to benzodiazepines was found on mouse T cells and not on human ones.
    primary_references
    [gb-p22927941] Different subtypes of GABA-A receptors are expressed in human, mouse and rat T lymphocytes. (2012). https://pubmed.ncbi.nlm.nih.gov/22927941/ DOI: 10.1371/journal.pone.0042959
    tissue_or_cell_type
    CD4 and CD8 T lymphocytes

    GABA: a ligand with no sign of its own, the cofactor that limits its synthesis, the barrier that keeps it out of the brain, and the immune settings where the same molecule protects and harms (2026-09-22) · lines 521–532

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Subunit isoform survey with immunoblotting, immunocytochemistry and whole-cell recording across three species · source_derived_draft · unverified_draft

    ### gb-the-subunits-differ-by-species There were 5, 8 and 13 different GABA-A subunit isoforms identified in human, mouse and rat CD4-positive and CD8-positive T cells respectively, importantly the gamma2 subunit that imposes benzodiazepine sensitivity on the GABA-A receptors was only detected in the mouse T cells, immunoblots and immunocytochemistry showed abundant GABA-A channel proteins in T cells from all three species, GABA-activated whole-cell transient and tonic currents were recorded and were inhibited by picrotoxin, SR95531 and bicuculline, and it is important to bear in mind the interspecies difference when selecting the appropriate animal models and when selecting drugs aimed at modulating human T cell function. Condition category: normal nutrient_topic: GABA research collection; topical membership is not evidence of a direct clinical effect, and the sign of a GABA response depends on the chloride gradient of the cell it was measured in. plain_language: The subunit that makes a receptor respond to benzodiazepines was found on mouse T cells and not on human ones. organism: Human, mouse and rat tissue_or_cell_type: CD4 and CD8 T lymphocytes experimental_model: Subunit isoform survey with immunoblotting, immunocytochemistry and whole-cell recording across three species limitations: The species comparison is the point. Subunit repertoires differ enough that a drug result in one species does not carry to another. exposure: GABA-activated transient and tonic currents, with picrotoxin, SR95531 and bicuculline evidence_span: {"source_cache": "artifacts/gaba-research/22927941.abstract.txt", "locator": "Indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "0457001832d2a3595899415221b7797f5288a787c1d52fd6afa1acf24f032402", "start_char": 0, "end_char": 1589, "text_sha256": "0457001832d2a3595899415221b7797f5288a787c1d52fd6afa1acf24f032402"} [gb-p22927941] Different subtypes of GABA-A receptors are expressed in human, mouse and rat T lymphocytes. (2012). https://pubmed.ncbi.nlm.nih.gov/22927941/ DOI: 10.1371/journal.pone.0042959
    Complete structured claim and evidence

Where it participates (unsigned role)

  1. We examined in encephalitogenic T cells if they expressed functional GABA channels that could be activated by the low nanomolar to 1 micromolar physiological concentrations of GABA present around neurons in the brain, the cells expressed the alpha1, alpha4, beta2, beta3, gamma1 and delta GABA-A channel subunits and formed functional extrasynaptic-like GABA channels that were activated by 1 micromolar GABA, and 100 nanomolar and higher GABA concentrations decreased T cell proliferation.

    Gamma-aminobutyric acid → T-cell proliferation, model specified source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/gaba-research/18954912.abstract.txt", "locator": "Indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "3723b5b4b643817af252230d67dafbc0b5ca2deadb17cdbdbc9f340ddfc6a472", "start_char": 0, "end_char": 728, "text_sha256": "3723b5b4b643817af252230d67dafbc0b5ca2deadb17cdbdbc9f340ddfc6a472"}
    experimental_model
    Patch clamp and proliferation assays on encephalitogenic T cells at physiological GABA concentrations
    exposure
    GABA at nanomolar to micromolar concentrations, the range present around neurons
    limitations
    A small subunit and function study in one T cell type. It measures the concentration range that matters rather than assuming it.
    nutrient_topic
    GABA research collection; topical membership is not evidence of a direct clinical effect, and the sign of a GABA response depends on the chloride gradient of the cell it was measured in. · Gamma-aminobutyric acid
    organism
    Rat
    plain_language
    These cells carry the kind of channel built for a steady background level rather than a synaptic pulse, and that background level slows them down.
    primary_references
    [gb-p18954912] GABA, a natural immunomodulator of T lymphocytes. (2008). https://pubmed.ncbi.nlm.nih.gov/18954912/ DOI: 10.1016/j.jneuroim.2008.08.017
    tissue_or_cell_type
    Encephalitogenic T cell

    GABA: a ligand with no sign of its own, the cofactor that limits its synthesis, the barrier that keeps it out of the brain, and the immune settings where the same molecule protects and harms (2026-09-22) · lines 508–519

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Patch clamp and proliferation assays on encephalitogenic T cells at physiological GABA concentrations · source_derived_draft · unverified_draft

    ### gb-t-cells-read-ambient-gaba We examined in encephalitogenic T cells if they expressed functional GABA channels that could be activated by the low nanomolar to 1 micromolar physiological concentrations of GABA present around neurons in the brain, the cells expressed the alpha1, alpha4, beta2, beta3, gamma1 and delta GABA-A channel subunits and formed functional extrasynaptic-like GABA channels that were activated by 1 micromolar GABA, and 100 nanomolar and higher GABA concentrations decreased T cell proliferation. Condition category: normal nutrient_topic: GABA research collection; topical membership is not evidence of a direct clinical effect, and the sign of a GABA response depends on the chloride gradient of the cell it was measured in. plain_language: These cells carry the kind of channel built for a steady background level rather than a synaptic pulse, and that background level slows them down. organism: Rat tissue_or_cell_type: Encephalitogenic T cell experimental_model: Patch clamp and proliferation assays on encephalitogenic T cells at physiological GABA concentrations limitations: A small subunit and function study in one T cell type. It measures the concentration range that matters rather than assuming it. exposure: GABA at nanomolar to micromolar concentrations, the range present around neurons evidence_span: {"source_cache": "artifacts/gaba-research/18954912.abstract.txt", "locator": "Indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "3723b5b4b643817af252230d67dafbc0b5ca2deadb17cdbdbc9f340ddfc6a472", "start_char": 0, "end_char": 728, "text_sha256": "3723b5b4b643817af252230d67dafbc0b5ca2deadb17cdbdbc9f340ddfc6a472"} [gb-p18954912] GABA, a natural immunomodulator of T lymphocytes. (2008). https://pubmed.ncbi.nlm.nih.gov/18954912/ DOI: 10.1016/j.jneuroim.2008.08.017
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

    Evidence, AI assistance and curation standards