Component
DES1 siRNA reduction
Reduced DES1 expression after targeted siRNA in primary chicken Müller cultures.
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What it acts on
DES1-targeted siRNA reduced 11-cis-retinol formation from all-trans-retinol in homogenates of primary chicken Müller cultures compared with nonsilencing RNA.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- cross_nutrient
- false
- evidence_location
- Supplementary Figure 8; Results: RNAi knockdown
- experimental_model
- Targeted RNA interference with nonsilencing control
- exposure
- Approximately 50% DES1 expression reduction; retinol-substrate homogenate assay.
- limitations
- Neither complete gene deletion nor a physiological necessity test in an intact retina.
- nutrient
- Vitamin A · Vitamin A
- nutrient_topic
- Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect. · Vitamin A
- organism
- Gallus gallus
- plain_language
- Reducing DES1 lowered the culture preparation’s isomerase output.
- primary_references
- [vav-kaylor2013] Identification of DES1 as a vitamin A isomerase in Müller glial cells of the retina. (2013). https://pubmed.ncbi.nlm.nih.gov/23143414/ DOI: 10.1038/nchembio.1114
- tissue_or_cell_type
- primary Müller cultures
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Vitamin A expansion: reproduction, light-driven vision and shared mechanisms (2026-09-17) · lines 588–601
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Targeted RNA interference with nonsilencing control · source_derived_draft · unverified_draft
### vae-des1-sirna-isomerization DES1-targeted siRNA reduced 11-cis-retinol formation from all-trans-retinol in homogenates of primary chicken Müller cultures compared with nonsilencing RNA. Condition category: machinery_impairment nutrient_topic: Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect. plain_language: Reducing DES1 lowered the culture preparation’s isomerase output. organism: Gallus gallus tissue_or_cell_type: primary Müller cultures experimental_model: Targeted RNA interference with nonsilencing control limitations: Neither complete gene deletion nor a physiological necessity test in an intact retina. exposure: Approximately 50% DES1 expression reduction; retinol-substrate homogenate assay. cross_nutrient: false evidence_location: Supplementary Figure 8; Results: RNAi knockdown nutrient: Vitamin A [vav-kaylor2013] Identification of DES1 as a vitamin A isomerase in Müller glial cells of the retina. (2013). https://pubmed.ncbi.nlm.nih.gov/23143414/ DOI: 10.1038/nchembio.1114
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.