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(2013). https://pubmed.ncbi.nlm.nih.gov/23143414/ DOI: 10.1038/nchembio.1114","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"primary Müller cultures","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"fc7f1ed1-b035-5571-be5b-e66035afc90b","evidence_kind":"source_excerpt","locator":"Lines 588-601","start_line":588,"end_line":601,"excerpt":"### vae-des1-sirna-isomerization\nDES1-targeted siRNA reduced 11-cis-retinol formation from all-trans-retinol in homogenates of primary chicken Müller cultures compared with nonsilencing RNA.\nCondition category: machinery_impairment\nnutrient_topic: Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Reducing DES1 lowered the culture preparation’s isomerase output.\norganism: Gallus gallus\ntissue_or_cell_type: primary Müller cultures\nexperimental_model: Targeted RNA interference with nonsilencing control\nlimitations: Neither complete gene deletion nor a physiological necessity test in an intact retina.\nexposure: Approximately 50% DES1 expression reduction; retinol-substrate homogenate assay.\ncross_nutrient: false\nevidence_location: Supplementary Figure 8; Results: RNAi knockdown\nnutrient: Vitamin A\n[vav-kaylor2013] Identification of DES1 as a vitamin A isomerase in Müller glial cells of the retina. 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