Component
Decorin glycosaminoglycan modification
Decorin glycosaminoglycan modification. Experimental scope belongs to each linked claim.
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
D-galactose did not rescue the decorin glycosaminoglycan defect in the tested mouse Tmem165-knockout ATDC5 cells.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- cross_nutrient
- Mouse TMEM165 (affected_protein)
- evidence_span
- {"source_cache": "artifacts/manganese-glycosylation-sources/morelle2022.txt", "locator": "Results and Figure 5; decorin", "file_sha256": "91d05677c6d7bb4224f71742888e39533e3f130d59e6b2ffcae3e2b2c058e9aa", "start_char": 25939, "end_char": 27086, "text_sha256": "d94138936b018635104317fedba6c1298f0d54c6cd7b3f578b60000cea925bf5", "text_characters": 1147}
- experimental_model
- Tmem165-knockout mouse ATDC5 chondrogenic cells
- exposure
- Decorin-transfected ATDC5 cells: 1 micromolar MnCl2, 1 millimolar galactose or 1 millimolar xylose for 36 hours.
- limitations
- Decorin chondroitin-sulfate elongation in mouse chondrogenic cells. This is a separate model from the human HEK N/O-glycosylation assays and does not establish clinical cartilage rescue.
- nutrient_topic
- Manganese research collection; topical membership is not evidence of a direct dietary effect. · Manganese
- organism
- Mus musculus
- plain_language
- Improved N-glycosylation did not mean the proteoglycan defect was corrected.
- primary_references
- [mn-gly-morelle2022] Differential Effects of D-Galactose Supplementation on Golgi Glycosylation Defects in TMEM165 Deficiency. (2022). https://pubmed.ncbi.nlm.nih.gov/35693943/ DOI: 10.3389/fcell.2022.903953
- tissue_or_cell_type
- Mouse chondrogenic ATDC5 cells and secreted decorin
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Manganese: enzyme cofactors, glycosylation, transport and nutrient interactions (2026-09-17) · lines 824–836
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Tmem165-knockout mouse ATDC5 chondrogenic cells · source_derived_draft · unverified_draft
### mn-gly-gal-gag-null D-galactose did not rescue the decorin glycosaminoglycan defect in the tested mouse Tmem165-knockout ATDC5 cells. Condition category: machinery_impairment nutrient_topic: Manganese research collection; topical membership is not evidence of a direct dietary effect. plain_language: Improved N-glycosylation did not mean the proteoglycan defect was corrected. organism: Mus musculus tissue_or_cell_type: Mouse chondrogenic ATDC5 cells and secreted decorin experimental_model: Tmem165-knockout mouse ATDC5 chondrogenic cells limitations: Decorin chondroitin-sulfate elongation in mouse chondrogenic cells. This is a separate model from the human HEK N/O-glycosylation assays and does not establish clinical cartilage rescue. exposure: Decorin-transfected ATDC5 cells: 1 micromolar MnCl2, 1 millimolar galactose or 1 millimolar xylose for 36 hours. cross_nutrient: Mouse TMEM165 (affected_protein) evidence_span: {"source_cache": "artifacts/manganese-glycosylation-sources/morelle2022.txt", "locator": "Results and Figure 5; decorin", "file_sha256": "91d05677c6d7bb4224f71742888e39533e3f130d59e6b2ffcae3e2b2c058e9aa", "start_char": 25939, "end_char": 27086, "text_sha256": "d94138936b018635104317fedba6c1298f0d54c6cd7b3f578b60000cea925bf5", "text_characters": 1147} [mn-gly-morelle2022] Differential Effects of D-Galactose Supplementation on Golgi Glycosylation Defects in TMEM165 Deficiency. (2022). https://pubmed.ncbi.nlm.nih.gov/35693943/ DOI: 10.3389/fcell.2022.903953
Complete structured claim and evidenceMnCl2 restored the decorin glycosaminoglycan readout in mouse Tmem165-knockout ATDC5 cells.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- cross_nutrient
- Manganese(II) ion (supplied_ion); Mouse TMEM165 (affected_protein)
- evidence_span
- {"source_cache": "artifacts/manganese-glycosylation-sources/morelle2022.txt", "locator": "Results and Figure 5; decorin migration", "file_sha256": "91d05677c6d7bb4224f71742888e39533e3f130d59e6b2ffcae3e2b2c058e9aa", "start_char": 25939, "end_char": 27086, "text_sha256": "d94138936b018635104317fedba6c1298f0d54c6cd7b3f578b60000cea925bf5", "text_characters": 1147}
- experimental_model
- Tmem165-knockout mouse ATDC5 chondrogenic cells
- exposure
- Decorin-transfected ATDC5 cells: 1 micromolar MnCl2, 1 millimolar galactose or 1 millimolar xylose for 36 hours.
- limitations
- Decorin chondroitin-sulfate elongation in mouse chondrogenic cells. This is a separate model from the human HEK N/O-glycosylation assays and does not establish clinical cartilage rescue.
- nutrient_topic
- Manganese research collection; topical membership is not evidence of a direct dietary effect. · Manganese
- organism
- Mus musculus
- plain_language
- Manganese restored the measured sugar-chain modification on a matrix proteoglycan.
- primary_references
- [mn-gly-morelle2022] Differential Effects of D-Galactose Supplementation on Golgi Glycosylation Defects in TMEM165 Deficiency. (2022). https://pubmed.ncbi.nlm.nih.gov/35693943/ DOI: 10.3389/fcell.2022.903953
- tissue_or_cell_type
- Mouse chondrogenic ATDC5 cells and secreted decorin
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Manganese: enzyme cofactors, glycosylation, transport and nutrient interactions (2026-09-17) · lines 782–794
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Tmem165-knockout mouse ATDC5 chondrogenic cells · source_derived_draft · unverified_draft
### mn-gly-mn-gag-rescue MnCl2 restored the decorin glycosaminoglycan readout in mouse Tmem165-knockout ATDC5 cells. Condition category: machinery_impairment nutrient_topic: Manganese research collection; topical membership is not evidence of a direct dietary effect. plain_language: Manganese restored the measured sugar-chain modification on a matrix proteoglycan. organism: Mus musculus tissue_or_cell_type: Mouse chondrogenic ATDC5 cells and secreted decorin experimental_model: Tmem165-knockout mouse ATDC5 chondrogenic cells limitations: Decorin chondroitin-sulfate elongation in mouse chondrogenic cells. This is a separate model from the human HEK N/O-glycosylation assays and does not establish clinical cartilage rescue. exposure: Decorin-transfected ATDC5 cells: 1 micromolar MnCl2, 1 millimolar galactose or 1 millimolar xylose for 36 hours. cross_nutrient: Manganese(II) ion (supplied_ion); Mouse TMEM165 (affected_protein) evidence_span: {"source_cache": "artifacts/manganese-glycosylation-sources/morelle2022.txt", "locator": "Results and Figure 5; decorin migration", "file_sha256": "91d05677c6d7bb4224f71742888e39533e3f130d59e6b2ffcae3e2b2c058e9aa", "start_char": 25939, "end_char": 27086, "text_sha256": "d94138936b018635104317fedba6c1298f0d54c6cd7b3f578b60000cea925bf5", "text_characters": 1147} [mn-gly-morelle2022] Differential Effects of D-Galactose Supplementation on Golgi Glycosylation Defects in TMEM165 Deficiency. (2022). https://pubmed.ncbi.nlm.nih.gov/35693943/ DOI: 10.3389/fcell.2022.903953
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.