Component

Colonic thiamine diphosphate uptake

Measured apical uptake of ThDP by colonocytes or colonic tissue.

4 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What acts on it

  1. NCM460 colonocytes took up radiolabeled ThDP without prior substrate dephosphorylation; native human apical vesicles also showed carrier-mediated uptake.

    Thiamine diphosphate → Colonic thiamine diphosphate uptake source_derived_draftungraded
    Experimental context and source evidence
    evidence-scope
    Colonocytes; colonic apical membranes
    evidence_locator
    Abstract
    evidence_spans
    [{"source_document": "artifacts/thiamine_transport_sources/nabokina-2012-colonic-tpp-source-record.json", "source_field": "resultList.result[0].abstractText", "start_char": 0, "end_char": 1813}]
    experimental_model
    NCM460 cells and human donor colonic apical vesicles; custom tritiated ThDP.
    limitations
    Does not quantify systemic contribution from gut microbial B1.
    nutrient_topic
    Thiamine research collection; topical membership is not evidence of a direct dietary effect. · Thiamine (vitamin B1)
    organism
    Homo sapiens
    plain_language
    Colon cells can absorb the phosphorylated B1 form directly.
    primary_references
    [nabokina-2012-colonic-tpp] A high-affinity and specific carrier-mediated mechanism for uptake of thiamine pyrophosphate by human colonic epithelial cells (2012). https://pmc.ncbi.nlm.nih.gov/articles/PMC3423106/ DOI: 10.1152/ajpgi.00151.2012
    tissue_or_cell_type
    Colonocytes; colonic apical membranes
    transport_direction
    Luminal/apical compartment into colonocytes.

    Thiamine: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 502–515

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · NCM460 cells and human donor colonic apical vesicles; custom tritiated ThDP. · source_derived_draft · unverified_draft

    ### b1-colon-intact-thdp-uptake NCM460 colonocytes took up radiolabeled ThDP without prior substrate dephosphorylation; native human apical vesicles also showed carrier-mediated uptake. Condition category: normal nutrient_topic: Thiamine research collection; topical membership is not evidence of a direct dietary effect. plain_language: Colon cells can absorb the phosphorylated B1 form directly. organism: Homo sapiens tissue_or_cell_type: Colonocytes; colonic apical membranes experimental_model: NCM460 cells and human donor colonic apical vesicles; custom tritiated ThDP. limitations: Does not quantify systemic contribution from gut microbial B1. transport_direction: Luminal/apical compartment into colonocytes. evidence_spans: [{"source_document": "artifacts/thiamine_transport_sources/nabokina-2012-colonic-tpp-source-record.json", "source_field": "resultList.result[0].abstractText", "start_char": 0, "end_char": 1813}] evidence_locator: Abstract evidence-scope: Colonocytes; colonic apical membranes [nabokina-2012-colonic-tpp] A high-affinity and specific carrier-mediated mechanism for uptake of thiamine pyrophosphate by human colonic epithelial cells (2012). https://pmc.ncbi.nlm.nih.gov/articles/PMC3423106/ DOI: 10.1152/ajpgi.00151.2012
    Complete structured claim and evidence

  2. Human SLC44A4 isoform 1 expression increased ThDP uptake in ARPE19 cells.

    Human SLC44A4 isoform 1 → Colonic thiamine diphosphate uptake source_derived_draftungraded
    Experimental context and source evidence
    evidence-scope
    ARPE19; MDCK; human colon
    evidence_locator
    Figure 1
    evidence_spans
    [{"source_document": "artifacts/thiamine_transport_sources/PMC3924303.txt", "start_char": 19781, "end_char": 21596}]
    experimental_model
    Human SLC44A4 isoforms expressed in ARPE19 cells; polarized MDCK imaging.
    limitations
    Heterologous uptake; host dietary contribution unmeasured.
    nutrient_topic
    Thiamine research collection; topical membership is not evidence of a direct dietary effect. · Thiamine (vitamin B1)
    organism
    Human constructs; human and canine host cells
    plain_language
    This SLC44A4 form can carry phosphorylated B1.
    primary_references
    [nabokina-2014-slc44a4] Molecular identification and functional characterization of the human colonic thiamine pyrophosphate transporter (2014). https://pmc.ncbi.nlm.nih.gov/articles/PMC3924303/ DOI: 10.1074/jbc.M113.528257
    tissue_or_cell_type
    ARPE19; MDCK; human colon

    Thiamine: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 517–529

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Human SLC44A4 isoforms expressed in ARPE19 cells; polarized MDCK imaging. · source_derived_draft · unverified_draft

    ### b1-slc44a4-iso1-uptake Human SLC44A4 isoform 1 expression increased ThDP uptake in ARPE19 cells. Condition category: normal nutrient_topic: Thiamine research collection; topical membership is not evidence of a direct dietary effect. plain_language: This SLC44A4 form can carry phosphorylated B1. organism: Human constructs; human and canine host cells tissue_or_cell_type: ARPE19; MDCK; human colon experimental_model: Human SLC44A4 isoforms expressed in ARPE19 cells; polarized MDCK imaging. limitations: Heterologous uptake; host dietary contribution unmeasured. evidence_locator: Figure 1 evidence_spans: [{"source_document": "artifacts/thiamine_transport_sources/PMC3924303.txt", "start_char": 19781, "end_char": 21596}] evidence-scope: ARPE19; MDCK; human colon [nabokina-2014-slc44a4] Molecular identification and functional characterization of the human colonic thiamine pyrophosphate transporter (2014). https://pmc.ncbi.nlm.nih.gov/articles/PMC3924303/ DOI: 10.1074/jbc.M113.528257
    Complete structured claim and evidence

  3. Expressed SLC44A4 isoform 3 also supported ThDP uptake.

    Human SLC44A4 isoform 3 → Colonic thiamine diphosphate uptake source_derived_draftungraded
    Experimental context and source evidence
    evidence-scope
    ARPE19; MDCK; human colon
    evidence_locator
    Figure 10
    evidence_spans
    [{"source_document": "artifacts/thiamine_transport_sources/PMC3924303.txt", "start_char": 39610, "end_char": 40292}]
    experimental_model
    Human SLC44A4 isoforms expressed in ARPE19 cells; polarized MDCK imaging.
    limitations
    Not evidence for untested isoform 2.
    nutrient_topic
    Thiamine research collection; topical membership is not evidence of a direct dietary effect. · Thiamine (vitamin B1)
    organism
    Human constructs; human and canine host cells
    plain_language
    A second splice form retained transport activity.
    primary_references
    [nabokina-2014-slc44a4] Molecular identification and functional characterization of the human colonic thiamine pyrophosphate transporter (2014). https://pmc.ncbi.nlm.nih.gov/articles/PMC3924303/ DOI: 10.1074/jbc.M113.528257
    tissue_or_cell_type
    ARPE19; MDCK; human colon

    Thiamine: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 531–543

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Human SLC44A4 isoforms expressed in ARPE19 cells; polarized MDCK imaging. · source_derived_draft · unverified_draft

    ### b1-slc44a4-iso3-uptake Expressed SLC44A4 isoform 3 also supported ThDP uptake. Condition category: normal nutrient_topic: Thiamine research collection; topical membership is not evidence of a direct dietary effect. plain_language: A second splice form retained transport activity. organism: Human constructs; human and canine host cells tissue_or_cell_type: ARPE19; MDCK; human colon experimental_model: Human SLC44A4 isoforms expressed in ARPE19 cells; polarized MDCK imaging. limitations: Not evidence for untested isoform 2. evidence_locator: Figure 10 evidence_spans: [{"source_document": "artifacts/thiamine_transport_sources/PMC3924303.txt", "start_char": 39610, "end_char": 40292}] evidence-scope: ARPE19; MDCK; human colon [nabokina-2014-slc44a4] Molecular identification and functional characterization of the human colonic thiamine pyrophosphate transporter (2014). https://pmc.ncbi.nlm.nih.gov/articles/PMC3924303/ DOI: 10.1074/jbc.M113.528257
    Complete structured claim and evidence

  4. Slc44a4 knockout nearly eliminated carrier-mediated colonic radiolabeled-ThDP uptake in mice.

    Mouse Slc44a4-null genotype → Colonic thiamine diphosphate uptake source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    machinery_impairment Imported condition classification; unverified.
    evidence-scope
    Colon
    evidence_locator
    Abstract
    evidence_spans
    [{"source_document": "artifacts/thiamine_transport_sources/sabui-2024-slc44a4-knockout-source-record.json", "source_field": "resultList.result[0].abstractText", "start_char": 0, "end_char": 2031}]
    experimental_model
    Cre-Lox Slc44a4 knockout mice versus littermates; uptake and DSS challenge.
    limitations
    Does not quantify human systemic B1 supply.
    nutrient_topic
    Thiamine research collection; topical membership is not evidence of a direct dietary effect. · Thiamine (vitamin B1)
    organism
    Mus musculus
    plain_language
    The carrier accounts for most measured colonic uptake in this model.
    primary_references
    [sabui-2024-slc44a4-knockout] Effect of knocking out mouse Slc44a4 on colonic uptake of the microbiota-generated thiamine pyrophosphate and colon physiology (2024). https://pubmed.ncbi.nlm.nih.gov/38713615/ DOI: 10.1152/ajpgi.00065.2024
    tissue_or_cell_type
    Colon
    trigger_kind
    machinery_impairment Imported condition classification; unverified.

    Thiamine: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 560–572

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Cre-Lox Slc44a4 knockout mice versus littermates; uptake and DSS challenge. · source_derived_draft · unverified_draft

    ### b1-slc44a4-mouse-uptake-loss Slc44a4 knockout nearly eliminated carrier-mediated colonic radiolabeled-ThDP uptake in mice. Condition category: machinery_impairment nutrient_topic: Thiamine research collection; topical membership is not evidence of a direct dietary effect. plain_language: The carrier accounts for most measured colonic uptake in this model. organism: Mus musculus tissue_or_cell_type: Colon experimental_model: Cre-Lox Slc44a4 knockout mice versus littermates; uptake and DSS challenge. limitations: Does not quantify human systemic B1 supply. evidence_spans: [{"source_document": "artifacts/thiamine_transport_sources/sabui-2024-slc44a4-knockout-source-record.json", "source_field": "resultList.result[0].abstractText", "start_char": 0, "end_char": 2031}] evidence_locator: Abstract evidence-scope: Colon [sabui-2024-slc44a4-knockout] Effect of knocking out mouse Slc44a4 on colonic uptake of the microbiota-generated thiamine pyrophosphate and colon physiology (2024). https://pubmed.ncbi.nlm.nih.gov/38713615/ DOI: 10.1152/ajpgi.00065.2024
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

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