Component

Cellular riboflavin uptake

Transport of extracellular riboflavin into cells.

4 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What acts on it

  1. Purified RFVT3 proteoliposomes showed riboflavin-associated proton influx; D119/E145 mutagenesis identified determinants of pH-sensitive transport.

    Riboflavin transporter 3 / SLC52A3 → Cellular riboflavin uptake source_derived_draftungraded
    Experimental context and source evidence
    evidence_location
    Figure 4g-l and discussion
    experimental_model
    Purified human transporter proteoliposomes and mutagenesis
    exposure
    Riboflavin addition with proton-sensitive 9-aminoacridine readout.
    limitations
    Proton/riboflavin stoichiometry and exact residue-specific transport steps remain unresolved.
    nutrient_topic
    Riboflavin research collection; topical membership is not evidence of a direct dietary effect. · Riboflavin (vitamin B2)
    organism
    Homo sapiens protein in reconstituted membranes
    plain_language
    RFVT3 can connect riboflavin transport with proton movement.
    primary_references
    [transport-rfvt-structure-2025] Structure and transport mechanism of human riboflavin transporters (2025). https://pmc.ncbi.nlm.nih.gov/articles/PMC12044054/ DOI: 10.1038/s41467-025-59255-7
    tissue_or_cell_type
    Cell-free proteoliposomes

    Riboflavin: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 254–265

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Purified human transporter proteoliposomes and mutagenesis · source_derived_draft · unverified_draft

    ### transport-rfvt3-proton-coupling Purified RFVT3 proteoliposomes showed riboflavin-associated proton influx; D119/E145 mutagenesis identified determinants of pH-sensitive transport. Condition category: normal nutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect. plain_language: RFVT3 can connect riboflavin transport with proton movement. organism: Homo sapiens protein in reconstituted membranes tissue_or_cell_type: Cell-free proteoliposomes experimental_model: Purified human transporter proteoliposomes and mutagenesis limitations: Proton/riboflavin stoichiometry and exact residue-specific transport steps remain unresolved. exposure: Riboflavin addition with proton-sensitive 9-aminoacridine readout. evidence_location: Figure 4g-l and discussion [transport-rfvt-structure-2025] Structure and transport mechanism of human riboflavin transporters (2025). https://pmc.ncbi.nlm.nih.gov/articles/PMC12044054/ DOI: 10.1038/s41467-025-59255-7
    Complete structured claim and evidence

  2. SLC52A2 L123P variant decreased radiolabeled-riboflavin uptake compared with wild-type RFVT2 in transfected HEK293 cells.

    SLC52A2 L123P variant → Cellular riboflavin uptake source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    machinery_impairment Imported condition classification; unverified.
    evidence_location
    Figure 2a
    experimental_model
    Transient transporter expression with radiotracer uptake
    exposure
    10 nM [3H]riboflavin, 1 minute, pH 7.4, 37 C.
    limitations
    Single-variant expression does not reproduce all neural tissue effects.
    nutrient_topic
    Riboflavin research collection; topical membership is not evidence of a direct dietary effect. · Riboflavin (vitamin B2)
    organism
    Homo sapiens
    plain_language
    The disease-associated transporter variant imports less riboflavin.
    primary_references
    [transport-slc52a2-disease-2012] Impaired riboflavin transport due to missense mutations in SLC52A2 causes Brown-Vialetto-Van Laere syndrome (2012). https://pmc.ncbi.nlm.nih.gov/articles/PMC3470687/ DOI: 10.1007/s10545-012-9513-y
    tissue_or_cell_type
    HEK293 cells
    trigger_kind
    machinery_impairment Imported condition classification; unverified.

    Riboflavin: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 425–436

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Transient transporter expression with radiotracer uptake · source_derived_draft · unverified_draft

    ### transport-slc52a2-l123p-uptake SLC52A2 L123P variant decreased radiolabeled-riboflavin uptake compared with wild-type RFVT2 in transfected HEK293 cells. Condition category: machinery_impairment nutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect. plain_language: The disease-associated transporter variant imports less riboflavin. organism: Homo sapiens tissue_or_cell_type: HEK293 cells experimental_model: Transient transporter expression with radiotracer uptake limitations: Single-variant expression does not reproduce all neural tissue effects. exposure: 10 nM [3H]riboflavin, 1 minute, pH 7.4, 37 C. evidence_location: Figure 2a [transport-slc52a2-disease-2012] Impaired riboflavin transport due to missense mutations in SLC52A2 causes Brown-Vialetto-Van Laere syndrome (2012). https://pmc.ncbi.nlm.nih.gov/articles/PMC3470687/ DOI: 10.1007/s10545-012-9513-y
    Complete structured claim and evidence

  3. SLC52A2 L339P variant decreased radiolabeled-riboflavin uptake compared with wild-type RFVT2 in transfected HEK293 cells.

    SLC52A2 L339P variant → Cellular riboflavin uptake source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    machinery_impairment Imported condition classification; unverified.
    evidence_location
    Figure 2a
    experimental_model
    Transient transporter expression with radiotracer uptake
    exposure
    10 nM [3H]riboflavin, 1 minute, pH 7.4, 37 C.
    limitations
    Single-variant expression does not reproduce all neural tissue effects.
    nutrient_topic
    Riboflavin research collection; topical membership is not evidence of a direct dietary effect. · Riboflavin (vitamin B2)
    organism
    Homo sapiens
    plain_language
    The disease-associated transporter variant imports less riboflavin.
    primary_references
    [transport-slc52a2-disease-2012] Impaired riboflavin transport due to missense mutations in SLC52A2 causes Brown-Vialetto-Van Laere syndrome (2012). https://pmc.ncbi.nlm.nih.gov/articles/PMC3470687/ DOI: 10.1007/s10545-012-9513-y
    tissue_or_cell_type
    HEK293 cells
    trigger_kind
    machinery_impairment Imported condition classification; unverified.

    Riboflavin: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 438–449

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Transient transporter expression with radiotracer uptake · source_derived_draft · unverified_draft

    ### transport-slc52a2-l339p-uptake SLC52A2 L339P variant decreased radiolabeled-riboflavin uptake compared with wild-type RFVT2 in transfected HEK293 cells. Condition category: machinery_impairment nutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect. plain_language: The disease-associated transporter variant imports less riboflavin. organism: Homo sapiens tissue_or_cell_type: HEK293 cells experimental_model: Transient transporter expression with radiotracer uptake limitations: Single-variant expression does not reproduce all neural tissue effects. exposure: 10 nM [3H]riboflavin, 1 minute, pH 7.4, 37 C. evidence_location: Figure 2a [transport-slc52a2-disease-2012] Impaired riboflavin transport due to missense mutations in SLC52A2 causes Brown-Vialetto-Van Laere syndrome (2012). https://pmc.ncbi.nlm.nih.gov/articles/PMC3470687/ DOI: 10.1007/s10545-012-9513-y
    Complete structured claim and evidence

  4. RFVT2 W31S abolished measurable uptake despite detectable membrane expression in the study, separating transport failure from simple protein absence.

    SLC52A2 W31S variant → Cellular riboflavin uptake source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    machinery_impairment Imported condition classification; unverified.
    evidence_location
    Figure 5A-B
    experimental_model
    HEK293 transient expression, tracer uptake and membrane immunoblot
    exposure
    W31S expression compared with wild type.
    limitations
    Crude-membrane detection does not quantify correctly oriented surface protein.
    nutrient_topic
    Riboflavin research collection; topical membership is not evidence of a direct dietary effect. · Riboflavin (vitamin B2)
    organism
    Homo sapiens
    plain_language
    A transporter can reach the membrane yet fail to carry riboflavin.
    primary_references
    [transport-slc52a2-neuronopathy-2014] Treatable childhood neuronopathy caused by mutations in riboflavin transporter RFVT2 (2014). https://pmc.ncbi.nlm.nih.gov/articles/PMC3891447/ DOI: 10.1093/brain/awt315
    tissue_or_cell_type
    HEK293 cells
    trigger_kind
    machinery_impairment Imported condition classification; unverified.

    Riboflavin: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 464–475

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · HEK293 transient expression, tracer uptake and membrane immunoblot · source_derived_draft · unverified_draft

    ### transport-slc52a2-w31s-intrinsic-defect RFVT2 W31S abolished measurable uptake despite detectable membrane expression in the study, separating transport failure from simple protein absence. Condition category: machinery_impairment nutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect. plain_language: A transporter can reach the membrane yet fail to carry riboflavin. organism: Homo sapiens tissue_or_cell_type: HEK293 cells experimental_model: HEK293 transient expression, tracer uptake and membrane immunoblot limitations: Crude-membrane detection does not quantify correctly oriented surface protein. exposure: W31S expression compared with wild type. evidence_location: Figure 5A-B [transport-slc52a2-neuronopathy-2014] Treatable childhood neuronopathy caused by mutations in riboflavin transporter RFVT2 (2014). https://pmc.ncbi.nlm.nih.gov/articles/PMC3891447/ DOI: 10.1093/brain/awt315
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

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