{"id":"48f205eb-c574-5f26-8d46-4cefe5b81a7f","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:transport-rfvt3-proton-coupling","predicate":"couples-proton-entry-to","statement":"Purified RFVT3 proteoliposomes showed riboflavin-associated proton influx; D119/E145 mutagenesis identified determinants of pH-sensitive transport.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"5539bb06-a24a-5118-b754-37ba90e05766","mechanism_event_label":"RFVT3 can connect riboflavin transport with proton movement.","subject":{"id":"036b3828-9ad7-5a41-9e89-119cef5c3665","slug":"slc52a3","display_name":"Riboflavin transporter 3 / SLC52A3","entity_type_key":"protein"},"object":{"id":"4d513cb9-67ef-53b3-bf96-b35813191439","slug":"cellular-riboflavin-uptake","display_name":"Cellular riboflavin uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5539bb06-a24a-5118-b754-37ba90e05766","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:transport-rfvt3-proton-coupling-event","event_type":"biochemical_relationship","label":"RFVT3 can connect riboflavin transport with proton movement.","description":"Purified RFVT3 proteoliposomes showed riboflavin-associated proton influx; D119/E145 mutagenesis identified determinants of pH-sensitive transport.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"036b3828-9ad7-5a41-9e89-119cef5c3665","slug":"slc52a3","display_name":"Riboflavin transporter 3 / SLC52A3","entity_type_key":"protein"},"role":"transporter","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"86eb1eee-a8d1-539c-8c17-0911f69b6f1b","slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"4d513cb9-67ef-53b3-bf96-b35813191439","slug":"cellular-riboflavin-uptake","display_name":"Cellular riboflavin uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_location","value_text":"Figure 4g-l and discussion","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified human transporter proteoliposomes and mutagenesis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Riboflavin addition with proton-sensitive 9-aminoacridine readout.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Proton/riboflavin stoichiometry and exact residue-specific transport steps remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Riboflavin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens protein in reconstituted membranes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"RFVT3 can connect riboflavin transport with proton movement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[transport-rfvt-structure-2025] Structure and transport mechanism of human riboflavin transporters (2025). https://pmc.ncbi.nlm.nih.gov/articles/PMC12044054/ DOI: 10.1038/s41467-025-59255-7","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cell-free proteoliposomes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"57de49f2-0f1c-5b7c-b8f5-7801e11a8dfe","evidence_kind":"source_excerpt","locator":"Lines 254-265","start_line":254,"end_line":265,"excerpt":"### transport-rfvt3-proton-coupling\nPurified RFVT3 proteoliposomes showed riboflavin-associated proton influx; D119/E145 mutagenesis identified determinants of pH-sensitive transport.\nCondition category: normal\nnutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: RFVT3 can connect riboflavin transport with proton movement.\norganism: Homo sapiens protein in reconstituted membranes\ntissue_or_cell_type: Cell-free proteoliposomes\nexperimental_model: Purified human transporter proteoliposomes and mutagenesis\nlimitations: Proton/riboflavin stoichiometry and exact residue-specific transport steps remain unresolved.\nexposure: Riboflavin addition with proton-sensitive 9-aminoacridine readout.\nevidence_location: Figure 4g-l and discussion\n[transport-rfvt-structure-2025] Structure and transport mechanism of human riboflavin transporters (2025). https://pmc.ncbi.nlm.nih.gov/articles/PMC12044054/ DOI: 10.1038/s41467-025-59255-7","model_system":"Purified human transporter proteoliposomes and mutagenesis","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [transport-rfvt-structure-2025] Structure and transport mechanism of human riboflavin transporters (2025). https://pmc.ncbi.nlm.nih.gov/articles/PMC12044054/ DOI: 10.1038/s41467-025-59255-7","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f7c9578-82bf-5e2d-b5c4-72a79fb4f6af","stable_key":"import-548ab9d6-3a9b-5bed-879c-17d03813b636","title":"Riboflavin: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"680cb6bc8249877f2410f551807f10d390fdd719014137d7414ba3420e29228d","revision_id":"7a61e299-908d-5372-860b-99ed190f9d7a","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}