Component

Garlic-associated sulfide generation in cell-free thiol assays

Garlic-associated sulfide generation in cell-free thiol assays. Interpret through the linked study species, preparation, exposure and measured endpoint.

4 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What acts on it

  1. The tested thiol glutathione supported sulfide generation with garlic juice in a cell-free assay.

    GSH → Garlic-associated sulfide generation in cell-free thiol assays Recorded from sourceungraded
    Experimental context and source evidence
    evidence_access
    Primary full text available; selected claim-relevant methods, results, tables/figures and limitations reviewed. Supplemental proteome and all secondary findings are not exhaustively extracted.
    experimental_condition
    Garlic juice in buffer without reducing thiol present · Garlic juice tested in the 2007 erythrocyte and vessel study Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_condition
    Garlic juice in buffer without reducing thiol present · GSH Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_contrast
    {"intervention": "glutathione plus garlic juice", "comparator": "Garlic juice in buffer without reducing thiol", "endpoint": "The tested thiol glutathione supported sulfide generation with garlic juice in a cell-free assay.", "effect_direction": "increase", "combination": "joint", "conditions": [{"entity_slug": "glutathione", "state": "present"}, {"entity_slug": "garlic-juice-benavides-2007", "state": "present"}]} Explicit extracted experimental comparison; source-derived draft.
    experimental_model
    Cell-free 2 mM thiol with garlic juice; pH 7.35, 37 C, low-oxygen assay.
    interpretation_status
    Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.
    limitations
    Alternative thiols are not equivalent in rate/yield. NAC directly participates, so it is not a selective intracellular-GSH manipulation.
    plain_language
    The tested thiol glutathione supported sulfide generation with garlic juice in a cell-free assay.
    primary_references
    Hydrogen sulfide mediates the vasoactivity of garlic. | 2007 | DOI 10.1073/pnas.0705710104 | PMID 17951430 | https://pubmed.ncbi.nlm.nih.gov/17951430/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC2084282/ | https://doi.org/10.1073/pnas.0705710104
    source_locator
    Reviewed reference lines 81-81; exact primary location described in quoted passage where extracted.

    Allicin: detailed mechanisms of action (reviewed 5 October 2026) · lines 81–81

    Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication. · supports · Cell-free 2 mM thiol with garlic juice; pH 7.35, 37 C, low-oxygen assay. · Recorded from source · Recorded from source

    **Alternative thiols and the vascular null.** GSH, cysteine, homocysteine, and NAC each supported garlic-associated sulfide generation in cell-free assays. In rat aortic rings, added GSH aided detectable sulfide production, but its omission did not significantly alter the tested polysulfide-induced relaxation. Membrane and intracellular thiols therefore matter alongside the added reagent. NAC is a direct participant in this chemistry, not a selective way to raise cellular GSH. Results in deoxygenated red-cell assays and oxygen-controlled rat vessels are not human oral pharmacokinetics. [Benavides 2007](https://pmc.ncbi.nlm.nih.gov/articles/PMC2084282/)
    Complete structured claim and evidence

  2. The tested thiol homocysteine supported sulfide generation with garlic juice in a cell-free assay.

    Homocysteine → Garlic-associated sulfide generation in cell-free thiol assays Recorded from sourceungraded
    Experimental context and source evidence
    evidence_access
    Primary full text available; selected claim-relevant methods, results, tables/figures and limitations reviewed. Supplemental proteome and all secondary findings are not exhaustively extracted.
    experimental_condition
    Garlic juice in buffer without reducing thiol present · Garlic juice tested in the 2007 erythrocyte and vessel study Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_condition
    Garlic juice in buffer without reducing thiol present · Homocysteine Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_contrast
    {"intervention": "homocysteine plus garlic juice", "comparator": "Garlic juice in buffer without reducing thiol", "endpoint": "The tested thiol homocysteine supported sulfide generation with garlic juice in a cell-free assay.", "effect_direction": "increase", "combination": "joint", "conditions": [{"entity_slug": "homocysteine", "state": "present"}, {"entity_slug": "garlic-juice-benavides-2007", "state": "present"}]} Explicit extracted experimental comparison; source-derived draft.
    experimental_model
    Cell-free 2 mM thiol with garlic juice; pH 7.35, 37 C, low-oxygen assay.
    interpretation_status
    Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.
    limitations
    Alternative thiols are not equivalent in rate/yield. NAC directly participates, so it is not a selective intracellular-GSH manipulation.
    plain_language
    The tested thiol homocysteine supported sulfide generation with garlic juice in a cell-free assay.
    primary_references
    Hydrogen sulfide mediates the vasoactivity of garlic. | 2007 | DOI 10.1073/pnas.0705710104 | PMID 17951430 | https://pubmed.ncbi.nlm.nih.gov/17951430/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC2084282/ | https://doi.org/10.1073/pnas.0705710104
    source_locator
    Reviewed reference lines 81-81; exact primary location described in quoted passage where extracted.

    Allicin: detailed mechanisms of action (reviewed 5 October 2026) · lines 81–81

    Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication. · supports · Cell-free 2 mM thiol with garlic juice; pH 7.35, 37 C, low-oxygen assay. · Recorded from source · Recorded from source

    **Alternative thiols and the vascular null.** GSH, cysteine, homocysteine, and NAC each supported garlic-associated sulfide generation in cell-free assays. In rat aortic rings, added GSH aided detectable sulfide production, but its omission did not significantly alter the tested polysulfide-induced relaxation. Membrane and intracellular thiols therefore matter alongside the added reagent. NAC is a direct participant in this chemistry, not a selective way to raise cellular GSH. Results in deoxygenated red-cell assays and oxygen-controlled rat vessels are not human oral pharmacokinetics. [Benavides 2007](https://pmc.ncbi.nlm.nih.gov/articles/PMC2084282/)
    Complete structured claim and evidence

  3. The tested thiol l-cysteine supported sulfide generation with garlic juice in a cell-free assay.

    L-Cysteine → Garlic-associated sulfide generation in cell-free thiol assays Recorded from sourceungraded
    Experimental context and source evidence
    evidence_access
    Primary full text available; selected claim-relevant methods, results, tables/figures and limitations reviewed. Supplemental proteome and all secondary findings are not exhaustively extracted.
    experimental_condition
    Garlic juice in buffer without reducing thiol present · Garlic juice tested in the 2007 erythrocyte and vessel study Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_condition
    Garlic juice in buffer without reducing thiol present · L-Cysteine Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_contrast
    {"intervention": "l-cysteine plus garlic juice", "comparator": "Garlic juice in buffer without reducing thiol", "endpoint": "The tested thiol l-cysteine supported sulfide generation with garlic juice in a cell-free assay.", "effect_direction": "increase", "combination": "joint", "conditions": [{"entity_slug": "l-cysteine", "state": "present"}, {"entity_slug": "garlic-juice-benavides-2007", "state": "present"}]} Explicit extracted experimental comparison; source-derived draft.
    experimental_model
    Cell-free 2 mM thiol with garlic juice; pH 7.35, 37 C, low-oxygen assay.
    interpretation_status
    Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.
    limitations
    Alternative thiols are not equivalent in rate/yield. NAC directly participates, so it is not a selective intracellular-GSH manipulation.
    plain_language
    The tested thiol l-cysteine supported sulfide generation with garlic juice in a cell-free assay.
    primary_references
    Hydrogen sulfide mediates the vasoactivity of garlic. | 2007 | DOI 10.1073/pnas.0705710104 | PMID 17951430 | https://pubmed.ncbi.nlm.nih.gov/17951430/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC2084282/ | https://doi.org/10.1073/pnas.0705710104
    source_locator
    Reviewed reference lines 81-81; exact primary location described in quoted passage where extracted.

    Allicin: detailed mechanisms of action (reviewed 5 October 2026) · lines 81–81

    Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication. · supports · Cell-free 2 mM thiol with garlic juice; pH 7.35, 37 C, low-oxygen assay. · Recorded from source · Recorded from source

    **Alternative thiols and the vascular null.** GSH, cysteine, homocysteine, and NAC each supported garlic-associated sulfide generation in cell-free assays. In rat aortic rings, added GSH aided detectable sulfide production, but its omission did not significantly alter the tested polysulfide-induced relaxation. Membrane and intracellular thiols therefore matter alongside the added reagent. NAC is a direct participant in this chemistry, not a selective way to raise cellular GSH. Results in deoxygenated red-cell assays and oxygen-controlled rat vessels are not human oral pharmacokinetics. [Benavides 2007](https://pmc.ncbi.nlm.nih.gov/articles/PMC2084282/)
    Complete structured claim and evidence

  4. The tested thiol n-acetylcysteine supported sulfide generation with garlic juice in a cell-free assay.

    N-Acetyl-L-cysteine → Garlic-associated sulfide generation in cell-free thiol assays Recorded from sourceungraded
    Experimental context and source evidence
    evidence_access
    Primary full text available; selected claim-relevant methods, results, tables/figures and limitations reviewed. Supplemental proteome and all secondary findings are not exhaustively extracted.
    experimental_condition
    Garlic juice in buffer without reducing thiol present · Garlic juice tested in the 2007 erythrocyte and vessel study Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_condition
    Garlic juice in buffer without reducing thiol present · N-Acetyl-L-cysteine Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_contrast
    {"intervention": "n-acetylcysteine plus garlic juice", "comparator": "Garlic juice in buffer without reducing thiol", "endpoint": "The tested thiol n-acetylcysteine supported sulfide generation with garlic juice in a cell-free assay.", "effect_direction": "increase", "combination": "joint", "conditions": [{"entity_slug": "n-acetylcysteine", "state": "present"}, {"entity_slug": "garlic-juice-benavides-2007", "state": "present"}]} Explicit extracted experimental comparison; source-derived draft.
    experimental_model
    Cell-free 2 mM thiol with garlic juice; pH 7.35, 37 C, low-oxygen assay.
    interpretation_status
    Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.
    limitations
    Alternative thiols are not equivalent in rate/yield. NAC directly participates, so it is not a selective intracellular-GSH manipulation.
    plain_language
    The tested thiol n-acetylcysteine supported sulfide generation with garlic juice in a cell-free assay.
    primary_references
    Hydrogen sulfide mediates the vasoactivity of garlic. | 2007 | DOI 10.1073/pnas.0705710104 | PMID 17951430 | https://pubmed.ncbi.nlm.nih.gov/17951430/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC2084282/ | https://doi.org/10.1073/pnas.0705710104
    source_locator
    Reviewed reference lines 81-81; exact primary location described in quoted passage where extracted.

    Allicin: detailed mechanisms of action (reviewed 5 October 2026) · lines 81–81

    Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication. · supports · Cell-free 2 mM thiol with garlic juice; pH 7.35, 37 C, low-oxygen assay. · Recorded from source · Recorded from source

    **Alternative thiols and the vascular null.** GSH, cysteine, homocysteine, and NAC each supported garlic-associated sulfide generation in cell-free assays. In rat aortic rings, added GSH aided detectable sulfide production, but its omission did not significantly alter the tested polysulfide-induced relaxation. Membrane and intracellular thiols therefore matter alongside the added reagent. NAC is a direct participant in this chemistry, not a selective way to raise cellular GSH. Results in deoxygenated red-cell assays and oxygen-controlled rat vessels are not human oral pharmacokinetics. [Benavides 2007](https://pmc.ncbi.nlm.nih.gov/articles/PMC2084282/)
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.