{"id":"828b7726-27e6-5fd8-8bcd-bd19395af554","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:thiol-sulfide-homocysteine","predicate":"increases_in_recorded_experiment","statement":"The tested thiol homocysteine supported sulfide generation with garlic juice in a cell-free assay.","claim_class":"mechanistic","status":"source_recorded","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"2cf4e571-16ba-5263-9c7b-1707e7fc5718","mechanism_event_label":"The tested thiol homocysteine supported sulfide generation with garlic juice in a cell-free assay.","subject":{"id":"5b635b42-bc0d-5c54-aa94-9cf76cd0ed47","slug":"homocysteine","display_name":"Homocysteine","entity_type_key":"small_molecule"},"object":{"id":"d63a9ca7-df28-597b-8b35-dc8b941dd08a","slug":"cell-free-garlic-sulfide-production","display_name":"Garlic-associated sulfide generation in cell-free thiol assays","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"2cf4e571-16ba-5263-9c7b-1707e7fc5718","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:thiol-sulfide-homocysteine-event","event_type":"biochemical_relationship","label":"The tested thiol homocysteine supported sulfide generation with garlic juice in a cell-free assay.","description":"**Alternative thiols and the vascular null.** GSH, cysteine, homocysteine, and NAC each supported garlic-associated sulfide generation in cell-free assays. In rat aortic rings, added GSH aided detectable sulfide production, but its omission did not significantly alter the tested polysulfide-induced relaxation. Membrane and intracellular thiols therefore matter alongside the added reagent. NAC is a direct participant in this chemistry, not a selective way to raise cellular GSH. Results in deoxygenated red-cell assays and oxygen-controlled rat vessels are not human oral pharmacokinetics. [Benavides 2007](https://pmc.ncbi.nlm.nih.gov/articles/PMC2084282/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5b635b42-bc0d-5c54-aa94-9cf76cd0ed47","slug":"homocysteine","display_name":"Homocysteine","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"homocysteine plus garlic juice","sequence_order":0,"notes":""},{"entity":{"id":"d63a9ca7-df28-597b-8b35-dc8b941dd08a","slug":"cell-free-garlic-sulfide-production","display_name":"Garlic-associated sulfide generation in cell-free thiol assays","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"increase","sequence_order":1,"notes":""},{"entity":{"id":"400109ca-c4df-5310-b308-7ef47554080e","slug":"garlic-juice-benavides-2007","display_name":"Garlic juice tested in the 2007 erythrocyte and vessel study","entity_type_key":"botanical"},"role":"joint sulfur preparation","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"70cc3ced-8adc-5e76-9750-e1415683455b","slug":"hydrogen-sulfide","display_name":"Hydrogen sulfide / H2S","entity_type_key":"small_molecule"},"role":"measured free sulfide","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text available; selected claim-relevant methods, results, tables/figures and limitations reviewed. Supplemental proteome and all secondary findings are not exhaustively extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"present","comparator":"Garlic juice in buffer without reducing thiol","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"garlic-juice-benavides-2007","display_name":"Garlic juice tested in the 2007 erythrocyte and vessel study","entity_type_key":"botanical"}},{"dimension":"experimental_condition","value_text":"present","comparator":"Garlic juice in buffer without reducing thiol","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"homocysteine","display_name":"Homocysteine","entity_type_key":"small_molecule"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"homocysteine plus garlic juice\", \"comparator\": \"Garlic juice in buffer without reducing thiol\", \"endpoint\": \"The tested thiol homocysteine supported sulfide generation with garlic juice in a cell-free assay.\", \"effect_direction\": \"increase\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"homocysteine\", \"state\": \"present\"}, {\"entity_slug\": \"garlic-juice-benavides-2007\", \"state\": \"present\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Cell-free 2 mM thiol with garlic juice; pH 7.35, 37 C, low-oxygen assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Alternative thiols are not equivalent in rate/yield. NAC directly participates, so it is not a selective intracellular-GSH manipulation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The tested thiol homocysteine supported sulfide generation with garlic juice in a cell-free assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Hydrogen sulfide mediates the vasoactivity of garlic. | 2007 | DOI 10.1073/pnas.0705710104 | PMID 17951430 | https://pubmed.ncbi.nlm.nih.gov/17951430/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC2084282/ | https://doi.org/10.1073/pnas.0705710104","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 81-81; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6ea3624c-b28c-58fd-908c-c8552ff3bdea","evidence_kind":"source_excerpt","locator":"Lines 81-81","start_line":81,"end_line":81,"excerpt":"**Alternative thiols and the vascular null.** GSH, cysteine, homocysteine, and NAC each supported garlic-associated sulfide generation in cell-free assays. In rat aortic rings, added GSH aided detectable sulfide production, but its omission did not significantly alter the tested polysulfide-induced relaxation. Membrane and intracellular thiols therefore matter alongside the added reagent. NAC is a direct participant in this chemistry, not a selective way to raise cellular GSH. Results in deoxygenated red-cell assays and oxygen-controlled rat vessels are not human oral pharmacokinetics. [Benavides 2007](https://pmc.ncbi.nlm.nih.gov/articles/PMC2084282/)","model_system":"Cell-free 2 mM thiol with garlic juice; pH 7.35, 37 C, low-oxygen assay.","directness":"reported_statement","verification_status":"source_recorded","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. 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