Component
NADPH-dependent GSSA reduction by purified yeast glutathione reductase
NADPH-dependent GSSA reduction by purified yeast glutathione reductase. Interpret through the linked study species, preparation, exposure and measured endpoint.
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
Purified yeast glutathione reductase used GSSA as a substrate with NADPH consumption; apparent Km was 0.50 mM.
Experimental context and source evidence
- evidence_access
- Primary full text available; selected claim-relevant methods, results, tables/figures and limitations reviewed. Supplemental proteome and all secondary findings are not exhaustively extracted.
- experimental_model
- Purified commercial Saccharomyces cerevisiae enzyme; NADPH absorbance assay without DTNB.
- interpretation_status
- Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.
- limitations
- Not human GSR validation. GSSG comparator used a different assay, and Km alone does not measure catalytic efficiency.
- plain_language
- Purified yeast glutathione reductase used GSSA as a substrate with NADPH consumption; apparent Km was 0.50 mM.
- primary_references
- <i>S</i>-allylmercaptoglutathione Is a Substrate for Glutathione Reductase (E.C. 1.8.1.7) from Yeast (<i>Saccharomyces cerevisiae</i>). | 2018 | DOI 10.3390/antiox7070086 | PMID 29986384 | https://pubmed.ncbi.nlm.nih.gov/29986384/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC6070820/ | https://doi.org/10.3390/antiox7070086
- source_locator
- Reviewed reference lines 47-47; exact primary location described in quoted passage where extracted.
Allicin: detailed mechanisms of action (reviewed 5 October 2026) · lines 47–47
Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication. · supports · Purified commercial Saccharomyces cerevisiae enzyme; NADPH absorbance assay without DTNB. · source_derived_draft · unverified_draft
**GSSA recycling and NADPH.** Purified yeast glutathione reductase reduced GSSA with NADPH consumption. Reported apparent Km values were 0.50 mM for GSSA and 0.07 mM for GSSG. They do not establish an eightfold difference in catalytic efficiency: turnover and assay differences matter, and the GSSA measurement deliberately omitted the DTNB cycling reagent used for GSSG. GSSA also supported growth of glutathione-synthesis-defective yeast. That rescue is consistent with recovery of usable glutathione, but it is not a human GSR experiment or proof that this enzyme was the exclusive route in living cells. [Horn 2018](https://pmc.ncbi.nlm.nih.gov/articles/PMC6070820/)
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.