Component
Human WNK lysine deficient protein kinase 3 / WNK3
Human WNK lysine deficient protein kinase 3 / WNK3. Species, exposure and limitations are retained in each linked claim.
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
Chloride also opposed osmolyte-stimulated WNK3 autophosphorylation.
Experimental context and source evidence
- evidence_span
- {"source_cache": "artifacts/chloride-research/33689398.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "8a51e7a42ab9c614abf2c38e22f5d563d5416fffa11b7aec5919ee221a94e1b9", "start_char": 0, "end_char": 1308, "text_sha256": "8a51e7a42ab9c614abf2c38e22f5d563d5416fffa11b7aec5919ee221a94e1b9"}
- experimental_model
- Biochemistry and structural measurements
- exposure
- PEG400 or ethylene glycol osmotic challenge
- limitations
- Artificial osmolytes; supports additional regulation, not a universal chloride-only switch.
- nutrient_topic
- Chloride research collection; topical membership is not evidence of a direct dietary effect. · Chloride
- organism
- Human WNK1 and WNK3 constructs
- plain_language
- Related kinases can share this regulation while remaining separate proteins.
- primary_references
- [chloride-p33689398] Osmosensing by WNK Kinases. (2021). https://pubmed.ncbi.nlm.nih.gov/33689398/ DOI: 10.1091/mbc.e20-01-0089
- tissue_or_cell_type
- Purified kinase domains
Chloride: transport, acid-base balance, nutrient interactions and loss states (2026-09-17) · lines 354–365
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Biochemistry and structural measurements · source_derived_draft · unverified_draft
### chloride-wnk3-osmotic Chloride also opposed osmolyte-stimulated WNK3 autophosphorylation. Condition category: normal nutrient_topic: Chloride research collection; topical membership is not evidence of a direct dietary effect. plain_language: Related kinases can share this regulation while remaining separate proteins. organism: Human WNK1 and WNK3 constructs tissue_or_cell_type: Purified kinase domains experimental_model: Biochemistry and structural measurements limitations: Artificial osmolytes; supports additional regulation, not a universal chloride-only switch. exposure: PEG400 or ethylene glycol osmotic challenge evidence_span: {"source_cache": "artifacts/chloride-research/33689398.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "8a51e7a42ab9c614abf2c38e22f5d563d5416fffa11b7aec5919ee221a94e1b9", "start_char": 0, "end_char": 1308, "text_sha256": "8a51e7a42ab9c614abf2c38e22f5d563d5416fffa11b7aec5919ee221a94e1b9"} [chloride-p33689398] Osmosensing by WNK Kinases. (2021). https://pubmed.ncbi.nlm.nih.gov/33689398/ DOI: 10.1091/mbc.e20-01-0089
Complete structured claim and evidenceMutation of the corresponding WNK3 E314 site reduced potassium inhibition.
Experimental context and source evidence
- evidence_span
- {"source_cache": "artifacts/chloride-research/42186973.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "2499194159ba661c04fab5e89f45495e428fe64b36b14b81940fb42b52853afa", "start_char": 0, "end_char": 1266, "text_sha256": "2499194159ba661c04fab5e89f45495e428fe64b36b14b81940fb42b52853afa"}
- experimental_model
- Crystallography and mutagenesis
- exposure
- Cesium surrogate and potassium-inhibition assays
- limitations
- Surrogate binding plus functional mutagenesis; not dietary potassium dosing or replacement of chloride sensing.
- nutrient_topic
- Chloride research collection; topical membership is not evidence of a direct dietary effect. · Chloride
- organism
- Human WNK1/WNK3 constructs
- plain_language
- The direct potassium-sensitive route also has evidence in WNK3.
- primary_references
- [chloride-p42186973] Structural Basis for Potassium Inhibition of WNK Kinases. (2026). https://pubmed.ncbi.nlm.nih.gov/42186973/ DOI: 10.1021/acs.biochem.5c00825
- tissue_or_cell_type
- Purified kinase domains
Chloride: transport, acid-base balance, nutrient interactions and loss states (2026-09-17) · lines 380–391
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Crystallography and mutagenesis · source_derived_draft · unverified_draft
### chloride-wnk3-potassium Mutation of the corresponding WNK3 E314 site reduced potassium inhibition. Condition category: normal nutrient_topic: Chloride research collection; topical membership is not evidence of a direct dietary effect. plain_language: The direct potassium-sensitive route also has evidence in WNK3. organism: Human WNK1/WNK3 constructs tissue_or_cell_type: Purified kinase domains experimental_model: Crystallography and mutagenesis limitations: Surrogate binding plus functional mutagenesis; not dietary potassium dosing or replacement of chloride sensing. exposure: Cesium surrogate and potassium-inhibition assays evidence_span: {"source_cache": "artifacts/chloride-research/42186973.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "2499194159ba661c04fab5e89f45495e428fe64b36b14b81940fb42b52853afa", "start_char": 0, "end_char": 1266, "text_sha256": "2499194159ba661c04fab5e89f45495e428fe64b36b14b81940fb42b52853afa"} [chloride-p42186973] Structural Basis for Potassium Inhibition of WNK Kinases. (2026). https://pubmed.ncbi.nlm.nih.gov/42186973/ DOI: 10.1021/acs.biochem.5c00825
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.