Component
Circadian reporter period in human U2OS cells
Study-scoped entity; inspect species, exposure, model and limitations on each claim.
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
Caffeine lengthened the circadian reporter period dose-dependently in U2OS cells.
Experimental context and source evidence
- evidence_access
- Primary abstract
- experimental_model
- Human osteosarcoma cells with clock-gene luciferase reporters.
- limitations
- Cell period is not identical to human sleep duration.
- nutrient_topic
- Caffeine collection; salts, coffee, species and coexposure contexts retain their identities. · Caffeine
- plain_language
- The effect also reached a cellular clock model.
- primary_references
- Effects of caffeine on the human circadian clock in vivo and in vitro. · 2015 · https://pubmed.ncbi.nlm.nih.gov/26378246/ · DOI 10.1126/scitranslmed.aac5125
Caffeine: receptors, metabolism, nutrient interactions, adaptation and discovery questions (2026-09-18) · lines 84–90
AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text. · supports · Human osteosarcoma cells with clock-gene luciferase reporters. · source_derived_draft · unverified_draft
## caf-clock-cell The effect also reached a cellular clock model. Caffeine lengthened the circadian reporter period dose-dependently in U2OS cells. Model: Human osteosarcoma cells with clock-gene luciferase reporters. Limitations: Cell period is not identical to human sleep duration. Evidence access: Primary abstract Effects of caffeine on the human circadian clock in vivo and in vitro. · 2015 · https://pubmed.ncbi.nlm.nih.gov/26378246/ · DOI 10.1126/scitranslmed.aac5125
Complete structured claim and evidence
Where it participates (unsigned role)
A cAMP biosensor detected increased cAMP; pharmacological and knockdown experiments supported adenosine-receptor signaling rather than RyR or PDE perturbation as sufficient to explain the clock effect.
Experimental context and source evidence
- evidence_access
- Primary abstract
- experimental_model
- U2OS cAMP biosensor and pathway perturbations.
- limitations
- Does not exclude high-concentration PDE or calcium-channel effects in other systems.
- nutrient_topic
- Caffeine collection; salts, coffee, species and coexposure contexts retain their identities. · Caffeine
- plain_language
- The clock experiment identified a receptor-linked signaling route.
- primary_references
- Effects of caffeine on the human circadian clock in vivo and in vitro. · 2015 · https://pubmed.ncbi.nlm.nih.gov/26378246/ · DOI 10.1126/scitranslmed.aac5125
Caffeine: receptors, metabolism, nutrient interactions, adaptation and discovery questions (2026-09-18) · lines 92–98
AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text. · supports · U2OS cAMP biosensor and pathway perturbations. · source_derived_draft · unverified_draft
## caf-clock-camp The clock experiment identified a receptor-linked signaling route. A cAMP biosensor detected increased cAMP; pharmacological and knockdown experiments supported adenosine-receptor signaling rather than RyR or PDE perturbation as sufficient to explain the clock effect. Model: U2OS cAMP biosensor and pathway perturbations. Limitations: Does not exclude high-concentration PDE or calcium-channel effects in other systems. Evidence access: Primary abstract Effects of caffeine on the human circadian clock in vivo and in vitro. · 2015 · https://pubmed.ncbi.nlm.nih.gov/26378246/ · DOI 10.1126/scitranslmed.aac5125
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.