Component
Sulforaphane-cysteine conjugate / SFN-Cys
Sulforaphane-cysteine conjugate / SFN-Cys. Species, exposure and limitations are retained in each linked claim.
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What it acts on
SFN-Cys inhibited HDAC activity in vitro, whereas parent sulforaphane and SFN-GSH had little or no direct effect in the comparison.
Experimental context and source evidence
- evidence_span
- {"source_cache": "artifacts/sulforaphane-research/15313918.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "9478957b1513e25cb47f13d6a1aca0df542fc851d8eb4b9c6394010e90dbe83e", "start_char": 0, "end_char": 1861, "text_sha256": "9478957b1513e25cb47f13d6a1aca0df542fc851d8eb4b9c6394010e90dbe83e"}
- experimental_model
- Cell exposure, enzyme assays, metabolites and chromatin immunoprecipitation
- exposure
- Parent SFN, SFN-GSH, SFN-Cys and SFN-NAC; GST inhibition
- limitations
- High-level HDAC activity was measured without establishing universal isoform inhibition; cell effects are not cancer-treatment outcomes.
- nutrient_topic
- Sulforaphane research collection; topical membership is not evidence of a direct dietary effect. · Sulforaphane / SFN, stereochemistry specified per study
- organism
- Human HEK293 and HCT116 cells
- plain_language
- A breakdown product had a different activity from the parent molecule.
- primary_references
- [sulforaphane-p15313918] A novel mechanism of chemoprotection by sulforaphane: inhibition of histone deacetylase. (2004). https://pubmed.ncbi.nlm.nih.gov/15313918/ DOI: 10.1158/0008-5472.can-04-1326
- tissue_or_cell_type
- Histone deacetylase activity and histone acetylation
Sulforaphane: formation, electrophile sensing and nutrient connections (2026-09-17) · lines 567–578
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Cell exposure, enzyme assays, metabolites and chromatin immunoprecipitation · source_derived_draft · unverified_draft
### sulforaphane-cys-hdac SFN-Cys inhibited HDAC activity in vitro, whereas parent sulforaphane and SFN-GSH had little or no direct effect in the comparison. Condition category: normal nutrient_topic: Sulforaphane research collection; topical membership is not evidence of a direct dietary effect. plain_language: A breakdown product had a different activity from the parent molecule. organism: Human HEK293 and HCT116 cells tissue_or_cell_type: Histone deacetylase activity and histone acetylation experimental_model: Cell exposure, enzyme assays, metabolites and chromatin immunoprecipitation limitations: High-level HDAC activity was measured without establishing universal isoform inhibition; cell effects are not cancer-treatment outcomes. exposure: Parent SFN, SFN-GSH, SFN-Cys and SFN-NAC; GST inhibition evidence_span: {"source_cache": "artifacts/sulforaphane-research/15313918.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "9478957b1513e25cb47f13d6a1aca0df542fc851d8eb4b9c6394010e90dbe83e", "start_char": 0, "end_char": 1861, "text_sha256": "9478957b1513e25cb47f13d6a1aca0df542fc851d8eb4b9c6394010e90dbe83e"} [sulforaphane-p15313918] A novel mechanism of chemoprotection by sulforaphane: inhibition of histone deacetylase. (2004). https://pubmed.ncbi.nlm.nih.gov/15313918/ DOI: 10.1158/0008-5472.can-04-1326
Complete structured claim and evidence
What acts on it
Sulforaphane-cysteine was separately quantified in human urine after broccoli sprout exposure.
Experimental context and source evidence
- evidence_span
- {"source_cache": "artifacts/sulforaphane-research/18729326.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "1b30293ede979c158852939b48f68a2cd71fa605bbca813a9fe2325f99e5c1d3", "start_char": 0, "end_char": 2172, "text_sha256": "1b30293ede979c158852939b48f68a2cd71fa605bbca813a9fe2325f99e5c1d3"}
- experimental_model
- Isotope-dilution LC-MS/MS assay validation in intervention urines
- exposure
- Broccoli sprout intervention specimens with equivalent administered preparation
- limitations
- Analytical exposure study; metabolite concentration is not tissue efficacy and does not identify each responsible human enzyme.
- nutrient_topic
- Sulforaphane research collection; topical membership is not evidence of a direct dietary effect. · Sulforaphane / SFN, stereochemistry specified per study
- organism
- Human, 98 urine specimens
- plain_language
- This metabolite has its own identity and can be measured separately from the parent.
- primary_references
- [sulforaphane-p18729326] Quantification of sulforaphane mercapturic acid pathway conjugates in human urine by high-performance liquid chromatography and isotope-dilution tandem mass spectrometry. (2008). https://pubmed.ncbi.nlm.nih.gov/18729326/ DOI: 10.1021/tx800210k
- tissue_or_cell_type
- Urinary mercapturic-acid pathway species
Sulforaphane: formation, electrophile sensing and nutrient connections (2026-09-17) · lines 359–370
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Isotope-dilution LC-MS/MS assay validation in intervention urines · source_derived_draft · unverified_draft
### sulforaphane-urine-cys Sulforaphane-cysteine was separately quantified in human urine after broccoli sprout exposure. Condition category: normal nutrient_topic: Sulforaphane research collection; topical membership is not evidence of a direct dietary effect. plain_language: This metabolite has its own identity and can be measured separately from the parent. organism: Human, 98 urine specimens tissue_or_cell_type: Urinary mercapturic-acid pathway species experimental_model: Isotope-dilution LC-MS/MS assay validation in intervention urines limitations: Analytical exposure study; metabolite concentration is not tissue efficacy and does not identify each responsible human enzyme. exposure: Broccoli sprout intervention specimens with equivalent administered preparation evidence_span: {"source_cache": "artifacts/sulforaphane-research/18729326.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "1b30293ede979c158852939b48f68a2cd71fa605bbca813a9fe2325f99e5c1d3", "start_char": 0, "end_char": 2172, "text_sha256": "1b30293ede979c158852939b48f68a2cd71fa605bbca813a9fe2325f99e5c1d3"} [sulforaphane-p18729326] Quantification of sulforaphane mercapturic acid pathway conjugates in human urine by high-performance liquid chromatography and isotope-dilution tandem mass spectrometry. (2008). https://pubmed.ncbi.nlm.nih.gov/18729326/ DOI: 10.1021/tx800210k
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.