Component

Streptavidin

Streptavidin. Species, exposure and limitations are retained in each linked claim.

4 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What it acts on

  1. The 2008 assay study found biotin-independent streptavidin binding to histones and detected radiolabeled biotin on carboxylases but not histones.

    Streptavidin → Streptavidin binding in histone detection assays source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/biotin-research/17920026.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "4b20857841bd32babc7962b138c4e56b6502a92d3bd6443e83fc0be32308e26d", "start_char": 0, "end_char": 1357, "text_sha256": "4b20857841bd32babc7962b138c4e56b6502a92d3bd6443e83fc0be32308e26d"}
    experimental_model
    Cell-culture biotin depletion, streptavidin controls and radiolabeled-biotin incorporation
    exposure
    Assay-method comparison
    limitations
    Failure to detect is an upper bound, not proof of zero; the stated less-than-0.03% bound is compatible numerically with the later less-than-0.001% estimate.
    nutrient_topic
    Biotin research collection; topical membership is not evidence of a direct dietary effect. · Biotin
    organism
    Cultured cells as specified in the primary paper
    plain_language
    A histone band can arise from the detection reagent rather than a biotin attachment.
    primary_references
    [b7-p17920026] Artifactual detection of biotin on histones by streptavidin. (2008). https://pubmed.ncbi.nlm.nih.gov/17920026/ DOI: 10.1016/j.ab.2007.09.003
    tissue_or_cell_type
    Histones and carboxylases

    Biotin: carboxylases, recycling, deficiency and nutrient interactions (2026-09-17) · lines 1170–1181

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Cell-culture biotin depletion, streptavidin controls and radiolabeled-biotin incorporation · source_derived_draft · unverified_draft

    ### b7-histone-artifact The 2008 assay study found biotin-independent streptavidin binding to histones and detected radiolabeled biotin on carboxylases but not histones. Condition category: normal nutrient_topic: Biotin research collection; topical membership is not evidence of a direct dietary effect. plain_language: A histone band can arise from the detection reagent rather than a biotin attachment. organism: Cultured cells as specified in the primary paper tissue_or_cell_type: Histones and carboxylases experimental_model: Cell-culture biotin depletion, streptavidin controls and radiolabeled-biotin incorporation limitations: Failure to detect is an upper bound, not proof of zero; the stated less-than-0.03% bound is compatible numerically with the later less-than-0.001% estimate. exposure: Assay-method comparison evidence_span: {"source_cache": "artifacts/biotin-research/17920026.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "4b20857841bd32babc7962b138c4e56b6502a92d3bd6443e83fc0be32308e26d", "start_char": 0, "end_char": 1357, "text_sha256": "4b20857841bd32babc7962b138c4e56b6502a92d3bd6443e83fc0be32308e26d"} [b7-p17920026] Artifactual detection of biotin on histones by streptavidin. (2008). https://pubmed.ncbi.nlm.nih.gov/17920026/ DOI: 10.1016/j.ab.2007.09.003
    Complete structured claim and evidence

Where it participates (unsigned role)

  1. Excess specimen biotin can compete with biotinylated assay components for streptavidin capture.

    Biotin → Streptavidin capture in biotinylated immunoassays source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    biomarker_context Imported condition classification; unverified.
    evidence_span
    {"source_cache": "artifacts/biotin-research/28973622.fulltext.txt", "locator": "Exact primary full-text span; zero-based, end-exclusive Unicode character offsets", "file_sha256": "4ee7e5947d8391b7a0fa7601d8390147b99c5ff603b3462e9d1aa7f3db42b915", "start_char": 10835, "end_char": 11046, "text_sha256": "6961c53bc6d3c1e97eafba73060fb1498d9d349e555f112e7e3bceb48761dd0b"}
    experimental_model
    Nonrandomized crossover study of 6 healthy adults, 37 assays and 11 analytes
    exposure
    10 mg oral biotin daily for 7 days
    limitations
    Interference depends on assay architecture and version; this study does not establish current platform performance, a universal washout time or true hormone changes.
    nutrient_topic
    Biotin research collection; topical membership is not evidence of a direct dietary effect. · Biotin
    organism
    Homo sapiens
    plain_language
    The supplement can interfere with the measuring tool itself.
    primary_references
    [b7-p28973622] Association of Biotin Ingestion With Performance of Hormone and Nonhormone Assays in Healthy Adults. (2017). https://pubmed.ncbi.nlm.nih.gov/28973622/ DOI: 10.1001/jama.2017.13705
    tissue_or_cell_type
    Blood specimens measured on named 2017 assay systems
    trigger_kind
    biomarker_context Imported condition classification; unverified.

    Biotin: carboxylases, recycling, deficiency and nutrient interactions (2026-09-17) · lines 1222–1233

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Nonrandomized crossover study of 6 healthy adults, 37 assays and 11 analytes · source_derived_draft · unverified_draft

    ### b7-assay-capture Excess specimen biotin can compete with biotinylated assay components for streptavidin capture. Condition category: biomarker_context nutrient_topic: Biotin research collection; topical membership is not evidence of a direct dietary effect. plain_language: The supplement can interfere with the measuring tool itself. organism: Homo sapiens tissue_or_cell_type: Blood specimens measured on named 2017 assay systems experimental_model: Nonrandomized crossover study of 6 healthy adults, 37 assays and 11 analytes limitations: Interference depends on assay architecture and version; this study does not establish current platform performance, a universal washout time or true hormone changes. exposure: 10 mg oral biotin daily for 7 days evidence_span: {"source_cache": "artifacts/biotin-research/28973622.fulltext.txt", "locator": "Exact primary full-text span; zero-based, end-exclusive Unicode character offsets", "file_sha256": "4ee7e5947d8391b7a0fa7601d8390147b99c5ff603b3462e9d1aa7f3db42b915", "start_char": 10835, "end_char": 11046, "text_sha256": "6961c53bc6d3c1e97eafba73060fb1498d9d349e555f112e7e3bceb48761dd0b"} [b7-p28973622] Association of Biotin Ingestion With Performance of Hormone and Nonhormone Assays in Healthy Adults. (2017). https://pubmed.ncbi.nlm.nih.gov/28973622/ DOI: 10.1001/jama.2017.13705
    Complete structured claim and evidence

  2. Five of eight biotinylated competitive assays produced falsely higher results after the studied biotin regimen.

    Biotin → Competitive immunoassay reported result source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    biomarker_context Imported condition classification; unverified.
    evidence_span
    {"source_cache": "artifacts/biotin-research/28973622.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "522d4d4aa94ca55b26c48c5b04c23de73b9c9fc70d6e7bcf400ffa81c77b2beb", "start_char": 0, "end_char": 2478, "text_sha256": "522d4d4aa94ca55b26c48c5b04c23de73b9c9fc70d6e7bcf400ffa81c77b2beb"}
    experimental_model
    Nonrandomized crossover study of 6 healthy adults, 37 assays and 11 analytes
    exposure
    10 mg oral biotin daily for 7 days
    limitations
    Interference depends on assay architecture and version; this study does not establish current platform performance, a universal washout time or true hormone changes.
    nutrient_topic
    Biotin research collection; topical membership is not evidence of a direct dietary effect. · Biotin
    organism
    Homo sapiens
    plain_language
    Other assay designs can produce the opposite error.
    primary_references
    [b7-p28973622] Association of Biotin Ingestion With Performance of Hormone and Nonhormone Assays in Healthy Adults. (2017). https://pubmed.ncbi.nlm.nih.gov/28973622/ DOI: 10.1001/jama.2017.13705
    tissue_or_cell_type
    Blood specimens measured on named 2017 assay systems
    trigger_kind
    biomarker_context Imported condition classification; unverified.

    Biotin: carboxylases, recycling, deficiency and nutrient interactions (2026-09-17) · lines 1248–1259

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Nonrandomized crossover study of 6 healthy adults, 37 assays and 11 analytes · source_derived_draft · unverified_draft

    ### b7-assay-competitive Five of eight biotinylated competitive assays produced falsely higher results after the studied biotin regimen. Condition category: biomarker_context nutrient_topic: Biotin research collection; topical membership is not evidence of a direct dietary effect. plain_language: Other assay designs can produce the opposite error. organism: Homo sapiens tissue_or_cell_type: Blood specimens measured on named 2017 assay systems experimental_model: Nonrandomized crossover study of 6 healthy adults, 37 assays and 11 analytes limitations: Interference depends on assay architecture and version; this study does not establish current platform performance, a universal washout time or true hormone changes. exposure: 10 mg oral biotin daily for 7 days evidence_span: {"source_cache": "artifacts/biotin-research/28973622.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "522d4d4aa94ca55b26c48c5b04c23de73b9c9fc70d6e7bcf400ffa81c77b2beb", "start_char": 0, "end_char": 2478, "text_sha256": "522d4d4aa94ca55b26c48c5b04c23de73b9c9fc70d6e7bcf400ffa81c77b2beb"} [b7-p28973622] Association of Biotin Ingestion With Performance of Hormone and Nonhormone Assays in Healthy Adults. (2017). https://pubmed.ncbi.nlm.nih.gov/28973622/ DOI: 10.1001/jama.2017.13705
    Complete structured claim and evidence

  3. Four of 15 biotinylated sandwich assays produced falsely lower results after the studied biotin regimen.

    Biotin → Sandwich immunoassay reported result source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    biomarker_context Imported condition classification; unverified.
    evidence_span
    {"source_cache": "artifacts/biotin-research/28973622.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "522d4d4aa94ca55b26c48c5b04c23de73b9c9fc70d6e7bcf400ffa81c77b2beb", "start_char": 0, "end_char": 2478, "text_sha256": "522d4d4aa94ca55b26c48c5b04c23de73b9c9fc70d6e7bcf400ffa81c77b2beb"}
    experimental_model
    Nonrandomized crossover study of 6 healthy adults, 37 assays and 11 analytes
    exposure
    10 mg oral biotin daily for 7 days
    limitations
    Interference depends on assay architecture and version; this study does not establish current platform performance, a universal washout time or true hormone changes.
    nutrient_topic
    Biotin research collection; topical membership is not evidence of a direct dietary effect. · Biotin
    organism
    Homo sapiens
    plain_language
    Some tests can read too low even though the substance in the body did not fall.
    primary_references
    [b7-p28973622] Association of Biotin Ingestion With Performance of Hormone and Nonhormone Assays in Healthy Adults. (2017). https://pubmed.ncbi.nlm.nih.gov/28973622/ DOI: 10.1001/jama.2017.13705
    tissue_or_cell_type
    Blood specimens measured on named 2017 assay systems
    trigger_kind
    biomarker_context Imported condition classification; unverified.

    Biotin: carboxylases, recycling, deficiency and nutrient interactions (2026-09-17) · lines 1235–1246

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Nonrandomized crossover study of 6 healthy adults, 37 assays and 11 analytes · source_derived_draft · unverified_draft

    ### b7-assay-sandwich Four of 15 biotinylated sandwich assays produced falsely lower results after the studied biotin regimen. Condition category: biomarker_context nutrient_topic: Biotin research collection; topical membership is not evidence of a direct dietary effect. plain_language: Some tests can read too low even though the substance in the body did not fall. organism: Homo sapiens tissue_or_cell_type: Blood specimens measured on named 2017 assay systems experimental_model: Nonrandomized crossover study of 6 healthy adults, 37 assays and 11 analytes limitations: Interference depends on assay architecture and version; this study does not establish current platform performance, a universal washout time or true hormone changes. exposure: 10 mg oral biotin daily for 7 days evidence_span: {"source_cache": "artifacts/biotin-research/28973622.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "522d4d4aa94ca55b26c48c5b04c23de73b9c9fc70d6e7bcf400ffa81c77b2beb", "start_char": 0, "end_char": 2478, "text_sha256": "522d4d4aa94ca55b26c48c5b04c23de73b9c9fc70d6e7bcf400ffa81c77b2beb"} [b7-p28973622] Association of Biotin Ingestion With Performance of Hormone and Nonhormone Assays in Healthy Adults. (2017). https://pubmed.ncbi.nlm.nih.gov/28973622/ DOI: 10.1001/jama.2017.13705
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

    Evidence, AI assistance and curation standards