Component
Human ZnT2 long isoform (NP_001004434)
Human SLC30A2 long protein isoform, the sequence reference for W152R and S296L numbering in PMID 23741301.
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
Where it participates (unsigned role)
Human ZnT2 S296L retained zinc transport and dimer-forming capacity in the DT40 characterization but had markedly reduced protein stability.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- cross_nutrient
- false
- experimental_model
- Human ZnT2 variants in zinc-sensitive DT40 cells; sequence reference NP_001004434
- exposure
- S296L variant compared with wild type.
- limitations
- Intrinsic activity and protein abundance must be separated; the variant is not described as completely transport-inactive.
- nutrient_topic
- Zinc research collection; topical membership is not evidence of a direct dietary effect. · Zinc
- organism
- Human protein in Gallus gallus cells
- plain_language
- Another ZnT2 variant could work when present, but was unstable.
- primary_references
- [zinc-trans-23741301] Compound heterozygous mutations in SLC30A2/ZnT2 results in low milk zinc concentrations: a novel mechanism for zinc deficiency in a breast-fed infant. (2013). https://pubmed.ncbi.nlm.nih.gov/23741301/ DOI: 10.1371/journal.pone.0064045
- tissue_or_cell_type
- Engineered DT40 cells
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17) · lines 349–360
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Human ZnT2 variants in zinc-sensitive DT40 cells; sequence reference NP_001004434 · source_derived_draft · unverified_draft
### zinc-trans-znt2-s296l-stability Human ZnT2 S296L retained zinc transport and dimer-forming capacity in the DT40 characterization but had markedly reduced protein stability. Condition category: machinery_impairment nutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect. plain_language: Another ZnT2 variant could work when present, but was unstable. organism: Human protein in Gallus gallus cells tissue_or_cell_type: Engineered DT40 cells experimental_model: Human ZnT2 variants in zinc-sensitive DT40 cells; sequence reference NP_001004434 limitations: Intrinsic activity and protein abundance must be separated; the variant is not described as completely transport-inactive. exposure: S296L variant compared with wild type. cross_nutrient: false [zinc-trans-23741301] Compound heterozygous mutations in SLC30A2/ZnT2 results in low milk zinc concentrations: a novel mechanism for zinc deficiency in a breast-fed infant. (2013). https://pubmed.ncbi.nlm.nih.gov/23741301/ DOI: 10.1371/journal.pone.0064045
Complete structured claim and evidenceHuman ZnT2 W152R lost zinc transport activity in zinc-sensitive DT40 cells.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- cross_nutrient
- false
- experimental_model
- Human ZnT2 constructs in zinc-sensitive chicken DT40 cells; sequence reference NP_001004434
- exposure
- W152R compared with wild type; functional complementation and zinc-handling assays.
- limitations
- Model tests intrinsic variant function; DT40 survival/compartment readouts are not direct measurement of zinc secretion into human milk.
- nutrient_topic
- Zinc research collection; topical membership is not evidence of a direct dietary effect. · Zinc
- organism
- Human protein in Gallus gallus cells
- plain_language
- One maternal ZnT2 variant could no longer move zinc in the test cells.
- primary_references
- [zinc-trans-23741301] Compound heterozygous mutations in SLC30A2/ZnT2 results in low milk zinc concentrations: a novel mechanism for zinc deficiency in a breast-fed infant. (2013). https://pubmed.ncbi.nlm.nih.gov/23741301/ DOI: 10.1371/journal.pone.0064045
- tissue_or_cell_type
- Engineered DT40-cell zinc compartments
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17) · lines 336–347
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Human ZnT2 constructs in zinc-sensitive chicken DT40 cells; sequence reference NP_001004434 · source_derived_draft · unverified_draft
### zinc-trans-znt2-w152r-transport Human ZnT2 W152R lost zinc transport activity in zinc-sensitive DT40 cells. Condition category: machinery_impairment nutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect. plain_language: One maternal ZnT2 variant could no longer move zinc in the test cells. organism: Human protein in Gallus gallus cells tissue_or_cell_type: Engineered DT40-cell zinc compartments experimental_model: Human ZnT2 constructs in zinc-sensitive chicken DT40 cells; sequence reference NP_001004434 limitations: Model tests intrinsic variant function; DT40 survival/compartment readouts are not direct measurement of zinc secretion into human milk. exposure: W152R compared with wild type; functional complementation and zinc-handling assays. cross_nutrient: false [zinc-trans-23741301] Compound heterozygous mutations in SLC30A2/ZnT2 results in low milk zinc concentrations: a novel mechanism for zinc deficiency in a breast-fed infant. (2013). https://pubmed.ncbi.nlm.nih.gov/23741301/ DOI: 10.1371/journal.pone.0064045
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.