Component
Human ZnT1 (SLC30A1)
Human plasma membrane zinc exporter protein.
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
Human ZnT1 purified with zinc at pH 6.0 yielded inward-facing, outward-facing and mixed dimers, whereas the pH 7.5 zinc-bound structure was outward-facing; the authors interpreted structures and simulations as supporting proton-dependent zinc release.
Experimental context and source evidence
- cross_nutrient
- false
- experimental_model
- Wild-type human ZnT1 cryo-EM, biochemical assays and molecular dynamics
- exposure
- Cryo-EM preparations with 1 mM zinc at pH 6.0 or pH 7.5.
- limitations
- Conformational snapshots and simulations do not directly quantify coupled proton flux or establish that calcium cannot couple transport. This record preserves observations and labels the interpretation.
- nutrient_topic
- Zinc research collection; topical membership is not evidence of a direct dietary effect. · Zinc
- organism
- Homo sapiens protein
- plain_language
- Acidity changed the shapes captured for human ZnT1, informing one proposed export mechanism.
- primary_references
- [zinc-trans-39390258] Structural insights into human zinc transporter ZnT1 mediated Zn2+ efflux. (2024). https://pubmed.ncbi.nlm.nih.gov/39390258/ DOI: 10.1038/s44319-024-00287-3
- tissue_or_cell_type
- Detergent-solubilized purified membrane protein
Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17) · lines 297–308
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Wild-type human ZnT1 cryo-EM, biochemical assays and molecular dynamics · source_derived_draft · unverified_draft
### zinc-trans-znt1-low-ph-conformations Human ZnT1 purified with zinc at pH 6.0 yielded inward-facing, outward-facing and mixed dimers, whereas the pH 7.5 zinc-bound structure was outward-facing; the authors interpreted structures and simulations as supporting proton-dependent zinc release. Condition category: normal nutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect. plain_language: Acidity changed the shapes captured for human ZnT1, informing one proposed export mechanism. organism: Homo sapiens protein tissue_or_cell_type: Detergent-solubilized purified membrane protein experimental_model: Wild-type human ZnT1 cryo-EM, biochemical assays and molecular dynamics limitations: Conformational snapshots and simulations do not directly quantify coupled proton flux or establish that calcium cannot couple transport. This record preserves observations and labels the interpretation. exposure: Cryo-EM preparations with 1 mM zinc at pH 6.0 or pH 7.5. cross_nutrient: false [zinc-trans-39390258] Structural insights into human zinc transporter ZnT1 mediated Zn2+ efflux. (2024). https://pubmed.ncbi.nlm.nih.gov/39390258/ DOI: 10.1038/s44319-024-00287-3
Complete structured claim and evidence
Where it participates (unsigned role)
In reconstituted human ZnT1 proteoliposomes, a transmembrane calcium gradient accelerated zinc transport and zinc addition drove calcium countertransport. Zinc transport persisted at a lower rate without the calcium gradient.
Experimental context and source evidence
- cross_nutrient
- true
- experimental_model
- Purified full-length human ZnT1 in proteoliposomes; supporting HEK293T transport assays
- exposure
- Proteoliposomes with or without internal calcium, external zinc titration; zinc transport Km 0.38 ± 0.14 micromolar in this assay.
- limitations
- In vitro transport direction depends on liposome gradients. This does not establish dietary calcium dependence or a fixed physiological Zn/Ca ratio. Calcium versus proton coupling remains incompletely resolved.
- nutrient_topic
- Zinc research collection; topical membership is not evidence of a direct dietary effect. · Zinc
- organism
- Homo sapiens protein
- plain_language
- A calcium gradient helped purified human ZnT1 move zinc; some movement remained without it.
- primary_references
- [zinc-trans-38669333] Structural insights into the calcium-coupled zinc export of human ZnT1. (2024). https://pubmed.ncbi.nlm.nih.gov/38669333/ DOI: 10.1126/sciadv.adk5128
- tissue_or_cell_type
- Reconstituted membrane; cultured-cell plasma membrane
Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17) · lines 284–295
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Purified full-length human ZnT1 in proteoliposomes; supporting HEK293T transport assays · source_derived_draft · unverified_draft
### zinc-trans-znt1-calcium-gradient In reconstituted human ZnT1 proteoliposomes, a transmembrane calcium gradient accelerated zinc transport and zinc addition drove calcium countertransport. Zinc transport persisted at a lower rate without the calcium gradient. Condition category: normal nutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect. plain_language: A calcium gradient helped purified human ZnT1 move zinc; some movement remained without it. organism: Homo sapiens protein tissue_or_cell_type: Reconstituted membrane; cultured-cell plasma membrane experimental_model: Purified full-length human ZnT1 in proteoliposomes; supporting HEK293T transport assays limitations: In vitro transport direction depends on liposome gradients. This does not establish dietary calcium dependence or a fixed physiological Zn/Ca ratio. Calcium versus proton coupling remains incompletely resolved. exposure: Proteoliposomes with or without internal calcium, external zinc titration; zinc transport Km 0.38 ± 0.14 micromolar in this assay. cross_nutrient: true [zinc-trans-38669333] Structural insights into the calcium-coupled zinc export of human ZnT1. (2024). https://pubmed.ncbi.nlm.nih.gov/38669333/ DOI: 10.1126/sciadv.adk5128
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.