Component
Human reduced folate carrier / SLC19A1
Human reduced folate carrier / SLC19A1; species and subcellular isoforms retained separately.
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What it acts on
Cryo-EM resolved 5-methyltetrahydrofolate in the central substrate pocket of human SLC19A1.
Experimental context and source evidence
- experimental_model
- Human SLC19A1 structures and HEK293F uptake assays
- exposure
- 5-MTHF-bound cryo-EM complex
- limitations
- Static inward-facing structure does not itself measure a complete transport cycle.
- nutrient_topic
- Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect. · Folate (vitamin B9)
- organism
- Homo sapiens
- plain_language
- RFC directly recognizes methylfolate.
- primary_references
- [dang2022] Molecular mechanism of substrate recognition by folate transporter SLC19A1 (2022). https://pubmed.ncbi.nlm.nih.gov/36575193/ DOI: 10.1038/s41421-022-00508-w
- tissue_or_cell_type
- Purified transporter and HEK293F cells
Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 155–165
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Human SLC19A1 structures and HEK293F uptake assays · source_derived_draft · unverified_draft
### folate-rfc-methylfolate-binding Cryo-EM resolved 5-methyltetrahydrofolate in the central substrate pocket of human SLC19A1. Condition category: normal nutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect. plain_language: RFC directly recognizes methylfolate. organism: Homo sapiens tissue_or_cell_type: Purified transporter and HEK293F cells experimental_model: Human SLC19A1 structures and HEK293F uptake assays limitations: Static inward-facing structure does not itself measure a complete transport cycle. exposure: 5-MTHF-bound cryo-EM complex [dang2022] Molecular mechanism of substrate recognition by folate transporter SLC19A1 (2022). https://pubmed.ncbi.nlm.nih.gov/36575193/ DOI: 10.1038/s41421-022-00508-w
Complete structured claim and evidence
What acts on it
Human SLC19A1 bound thiamine diphosphate in the same pocket as 5-MTHF; thiamine diphosphate inhibited methotrexate uptake with IC50 about 19 micromolar.
Experimental context and source evidence
- cross_nutrient
- B1-folate: phosphorylated thiamine and folates share RFC recognition; exclusive physiological counter-substrate status remains unproven.
- experimental_model
- Human SLC19A1 structures and HEK293F uptake assays
- exposure
- Binding assays and extracellular competition
- limitations
- Supports shared recognition; does not prove dietary B1 deficiency blocks folate delivery.
- nutrient_topic
- Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect. · Folate (vitamin B9)
- organism
- Homo sapiens
- plain_language
- A vitamin B1 cofactor can occupy the folate carrier.
- primary_references
- [dang2022] Molecular mechanism of substrate recognition by folate transporter SLC19A1 (2022). https://pubmed.ncbi.nlm.nih.gov/36575193/ DOI: 10.1038/s41421-022-00508-w
- tissue_or_cell_type
- Purified transporter and HEK293F cells
Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 167–178
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Human SLC19A1 structures and HEK293F uptake assays · source_derived_draft · unverified_draft
### folate-rfc-thdp-shared-pocket Human SLC19A1 bound thiamine diphosphate in the same pocket as 5-MTHF; thiamine diphosphate inhibited methotrexate uptake with IC50 about 19 micromolar. Condition category: normal nutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect. plain_language: A vitamin B1 cofactor can occupy the folate carrier. organism: Homo sapiens tissue_or_cell_type: Purified transporter and HEK293F cells experimental_model: Human SLC19A1 structures and HEK293F uptake assays limitations: Supports shared recognition; does not prove dietary B1 deficiency blocks folate delivery. exposure: Binding assays and extracellular competition cross_nutrient: B1-folate: phosphorylated thiamine and folates share RFC recognition; exclusive physiological counter-substrate status remains unproven. [dang2022] Molecular mechanism of substrate recognition by folate transporter SLC19A1 (2022). https://pubmed.ncbi.nlm.nih.gov/36575193/ DOI: 10.1038/s41421-022-00508-w
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.