Component
Human SDHAF2 Gly78Arg
Disease-associated SDHAF2 G78R protein studied in human-cell coimmunoprecipitation and yeast complementation.
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What it acts on
Human SDHAF2 G78R failed to restore Sdh1 flavination in sdh5-null yeast, whereas wild-type human SDHAF2 restored it to about 77% of wild-type yeast.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- evidence_spans
- [{"source_bundle": "artifacts/riboflavin_metabolism_sources.json", "source_key": "PMC3881419", "locator": "HTML article p", "paragraph_index": 22, "char_start": 0, "char_end": 998, "evidence_access": "full-text"}]
- experimental_model
- Yeast genetics, recombinant coexpression, human HEK293 binding assays and familial paraganglioma tissue.
- exposure
- Expression from yeast SDH5 promoter; normalized FAD fluorescence.
- limitations
- Cross-species complementation; not a riboflavin-supplement rescue experiment.
- nutrient_topic
- Riboflavin research collection; topical membership is not evidence of a direct dietary effect. · Riboflavin (vitamin B2)
- organism
- Homo sapiens protein in Saccharomyces cerevisiae
- plain_language
- The inherited altered assembly factor failed a functional rescue test.
- primary_references
- [hao-2009-sdh5] SDH5, a gene required for flavination of succinate dehydrogenase, is mutated in paraganglioma (2009). https://pubmed.ncbi.nlm.nih.gov/19628817/ DOI: 10.1126/science.1175689
- tissue_or_cell_type
- Yeast complementation
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Riboflavin: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 702–713
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Yeast genetics, recombinant coexpression, human HEK293 binding assays and familial paraganglioma tissue. · source_derived_draft · unverified_draft
### b2-met-sdhaf2-g78r-rescue Human SDHAF2 G78R failed to restore Sdh1 flavination in sdh5-null yeast, whereas wild-type human SDHAF2 restored it to about 77% of wild-type yeast. Condition category: machinery_impairment nutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect. plain_language: The inherited altered assembly factor failed a functional rescue test. organism: Homo sapiens protein in Saccharomyces cerevisiae tissue_or_cell_type: Yeast complementation experimental_model: Yeast genetics, recombinant coexpression, human HEK293 binding assays and familial paraganglioma tissue. limitations: Cross-species complementation; not a riboflavin-supplement rescue experiment. exposure: Expression from yeast SDH5 promoter; normalized FAD fluorescence. evidence_spans: [{"source_bundle": "artifacts/riboflavin_metabolism_sources.json", "source_key": "PMC3881419", "locator": "HTML article p", "paragraph_index": 22, "char_start": 0, "char_end": 998, "evidence_access": "full-text"}] [hao-2009-sdh5] SDH5, a gene required for flavination of succinate dehydrogenase, is mutated in paraganglioma (2009). https://pubmed.ncbi.nlm.nih.gov/19628817/ DOI: 10.1126/science.1175689
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.