Component
RGR K255A variant
Engineered RGR lysine-to-alanine variant numbered K255A in Zhang 2019; bovine construct in the curated assay.
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What it acts on
Bovine RGR K255A produced little 11-cis-retinal under illumination despite expression comparable to wild-type RGR in HEK293S GnTI-negative cells.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- cross_nutrient
- false
- evidence_location
- Results/Figure 7a-c
- experimental_model
- Recombinant bovine wild-type/K255A RGR comparison
- exposure
- All-trans-retinal and 530-nm light, with CRALBP; Figure 7.
- limitations
- Construct-specific assay; K255A numbering is retained from this paper.
- nutrient
- Vitamin A · Vitamin A
- nutrient_topic
- Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect. · Vitamin A
- organism
- Bos taurus protein in Homo sapiens cells
- plain_language
- Disrupting the retinal-binding lysine impaired the light-driven reaction.
- primary_references
- [vav-zhang2019] Photic generation of 11-cis-retinal in bovine retinal pigment epithelium. (2019). https://pubmed.ncbi.nlm.nih.gov/31694912/ DOI: 10.1074/jbc.ra119.011169
- tissue_or_cell_type
- HEK293S GnTI-negative homogenates
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Vitamin A expansion: reproduction, light-driven vision and shared mechanisms (2026-09-17) · lines 453–466
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Recombinant bovine wild-type/K255A RGR comparison · source_derived_draft · unverified_draft
### vae-rgr-k255a-photoactivity Bovine RGR K255A produced little 11-cis-retinal under illumination despite expression comparable to wild-type RGR in HEK293S GnTI-negative cells. Condition category: machinery_impairment nutrient_topic: Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect. plain_language: Disrupting the retinal-binding lysine impaired the light-driven reaction. organism: Bos taurus protein in Homo sapiens cells tissue_or_cell_type: HEK293S GnTI-negative homogenates experimental_model: Recombinant bovine wild-type/K255A RGR comparison limitations: Construct-specific assay; K255A numbering is retained from this paper. exposure: All-trans-retinal and 530-nm light, with CRALBP; Figure 7. cross_nutrient: false evidence_location: Results/Figure 7a-c nutrient: Vitamin A [vav-zhang2019] Photic generation of 11-cis-retinal in bovine retinal pigment epithelium. (2019). https://pubmed.ncbi.nlm.nih.gov/31694912/ DOI: 10.1074/jbc.ra119.011169
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.