Component
Respiratory-chain protein abundance
Proteomic abundance of electron-transport and ATP-synthesis protein clusters.
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
Three-day riboflavin starvation of mouse B16 cells reduced proteomic clusters associated with electron transport and ATP synthesis.
Experimental context and source evidence
- availability_state
- nutrient_deficiency Imported condition classification; unverified.
- evidence_spans
- [{"source_bundle": "artifacts/riboflavin_metabolism_sources.json", "source_key": "PMC7477094", "locator": "XML .//body//p", "paragraph_index": 21, "char_start": 0, "char_end": 3026, "evidence_access": "full-text"}]
- experimental_model
- Mouse B16 melanoma cells cultured in riboflavin-free medium for 3 days; quantitative proteomics.
- exposure
- Riboflavin-free culture for three days; quantitative proteomics.
- limitations
- Abundance, not direct pathway flux; cancer-cell dependence may differ from normal cells.
- nutrient_topic
- Riboflavin research collection; topical membership is not evidence of a direct dietary effect. · Riboflavin (vitamin B2)
- organism
- Mus musculus
- plain_language
- Removing B2 lowered the abundance of respiratory machinery in these tumor cells.
- primary_references
- [martinez-limon-2020-b2-depletion] Flavin dependency undermines proteome stability, lipid metabolism and cellular proliferation during vitamin B2 deficiency (2020). https://pubmed.ncbi.nlm.nih.gov/32895367/ DOI: 10.1038/s41419-020-02929-5
- tissue_or_cell_type
- B16 melanoma cells
- trigger_kind
- nutrient_deficiency Imported condition classification; unverified.
Riboflavin: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 970–981
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Mouse B16 melanoma cells cultured in riboflavin-free medium for 3 days; quantitative proteomics. · source_derived_draft · unverified_draft
### b2-met-b16-respiratory-proteome Three-day riboflavin starvation of mouse B16 cells reduced proteomic clusters associated with electron transport and ATP synthesis. Condition category: nutrient_deficiency nutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect. plain_language: Removing B2 lowered the abundance of respiratory machinery in these tumor cells. organism: Mus musculus tissue_or_cell_type: B16 melanoma cells experimental_model: Mouse B16 melanoma cells cultured in riboflavin-free medium for 3 days; quantitative proteomics. limitations: Abundance, not direct pathway flux; cancer-cell dependence may differ from normal cells. exposure: Riboflavin-free culture for three days; quantitative proteomics. evidence_spans: [{"source_bundle": "artifacts/riboflavin_metabolism_sources.json", "source_key": "PMC7477094", "locator": "XML .//body//p", "paragraph_index": 21, "char_start": 0, "char_end": 3026, "evidence_access": "full-text"}] [martinez-limon-2020-b2-depletion] Flavin dependency undermines proteome stability, lipid metabolism and cellular proliferation during vitamin B2 deficiency (2020). https://pubmed.ncbi.nlm.nih.gov/32895367/ DOI: 10.1038/s41419-020-02929-5
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.