Component
Rat N-terminal mini-cubilin construct
Rat cubilin N-terminal construct used by Udagawa 2018 (Methods: 1-928 amino acids; Figure 1 schematic: 1-930); retains N-terminus, EGF repeats and CUB1-4, not the complete IF binding region.
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
Human AMN coexpression allowed rat mini-cubilin to reach the plasma membrane in HEK293T and other tested renal/intestinal cell models, whereas mini-cubilin alone remained intracellular.
Experimental context and source evidence
- cross_nutrient
- false
- experimental_model
- Rat N-terminal mini-cubilin plus human AMN; HEK293T, MDCK, human RPTEC and HCT116
- exposure
- Recombinant coexpression versus single transfection
- limitations
- Mixed-species truncated construct lacks the complete CUB5-8 IF-binding region; surface expression is not direct B12 uptake.
- nutrient_topic
- Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect. · Vitamin B12 (cobalamins)
- organism
- Rattus norvegicus construct; Homo sapiens AMN; human/canine host cells
- plain_language
- Amnionless helped the experimental receptor reach the cell surface.
- primary_references
- [udagawa-2018-glycosylation] Amnionless-mediated glycosylation is crucial for cell surface targeting of cubilin in renal and intestinal cells. (2018). https://pubmed.ncbi.nlm.nih.gov/29402915/ DOI: 10.1038/s41598-018-20731-4
- tissue_or_cell_type
- Cultured renal and colorectal epithelial cells
Vitamin B12: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 322–333
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Rat N-terminal mini-cubilin plus human AMN; HEK293T, MDCK, human RPTEC and HCT116 · source_derived_draft · unverified_draft
### b12-abs-amn-trafficking-rat-construct Human AMN coexpression allowed rat mini-cubilin to reach the plasma membrane in HEK293T and other tested renal/intestinal cell models, whereas mini-cubilin alone remained intracellular. Condition category: normal nutrient_topic: Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect. plain_language: Amnionless helped the experimental receptor reach the cell surface. organism: Rattus norvegicus construct; Homo sapiens AMN; human/canine host cells tissue_or_cell_type: Cultured renal and colorectal epithelial cells experimental_model: Rat N-terminal mini-cubilin plus human AMN; HEK293T, MDCK, human RPTEC and HCT116 limitations: Mixed-species truncated construct lacks the complete CUB5-8 IF-binding region; surface expression is not direct B12 uptake. exposure: Recombinant coexpression versus single transfection cross_nutrient: false [udagawa-2018-glycosylation] Amnionless-mediated glycosylation is crucial for cell surface targeting of cubilin in renal and intestinal cells. (2018). https://pubmed.ncbi.nlm.nih.gov/29402915/ DOI: 10.1038/s41598-018-20731-4
Complete structured claim and evidence
Where it participates (unsigned role)
Combined N711D/N749D/N781D/N857D substitutions in rat mini-cubilin abolished mature glycosylation and surface targeting with human AMN; the tested smaller combinations did not.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- cross_nutrient
- false
- experimental_model
- Rat mini-cubilin 4567ND versus wild type in HEK293T cells coexpressing human AMN
- exposure
- Four Asn-to-Asp substitutions; Figure 6 and Supplementary Figure 11
- limitations
- Engineered combined construct; does not show that every single site is individually indispensable. B12 absorption itself was not measured.
- nutrient_topic
- Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect. · Vitamin B12 (cobalamins)
- organism
- Rattus norvegicus construct; Homo sapiens AMN and host cells
- plain_language
- Several glycosylation sites jointly support receptor maturation.
- primary_references
- [udagawa-2018-glycosylation] Amnionless-mediated glycosylation is crucial for cell surface targeting of cubilin in renal and intestinal cells. (2018). https://pubmed.ncbi.nlm.nih.gov/29402915/ DOI: 10.1038/s41598-018-20731-4
- tissue_or_cell_type
- HEK293T biosynthetic trafficking
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Vitamin B12: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 335–346
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Rat mini-cubilin 4567ND versus wild type in HEK293T cells coexpressing human AMN · source_derived_draft · unverified_draft
### b12-abs-glycosylation-quadruple-mutant Combined N711D/N749D/N781D/N857D substitutions in rat mini-cubilin abolished mature glycosylation and surface targeting with human AMN; the tested smaller combinations did not. Condition category: machinery_impairment nutrient_topic: Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect. plain_language: Several glycosylation sites jointly support receptor maturation. organism: Rattus norvegicus construct; Homo sapiens AMN and host cells tissue_or_cell_type: HEK293T biosynthetic trafficking experimental_model: Rat mini-cubilin 4567ND versus wild type in HEK293T cells coexpressing human AMN limitations: Engineered combined construct; does not show that every single site is individually indispensable. B12 absorption itself was not measured. exposure: Four Asn-to-Asp substitutions; Figure 6 and Supplementary Figure 11 cross_nutrient: false [udagawa-2018-glycosylation] Amnionless-mediated glycosylation is crucial for cell surface targeting of cubilin in renal and intestinal cells. (2018). https://pubmed.ncbi.nlm.nih.gov/29402915/ DOI: 10.1038/s41598-018-20731-4
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.