Component

Rat intestinal metallothioneins measured without isoform resolution

Rat intestinal metallothioneins measured without isoform resolution; model and exposure are recorded in linked claims.

3 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What acts on it

  1. The increased intestinal 64Cu was predominantly metallothionein-associated; the authors inferred copper displacement of zinc after zinc-induced metallothionein synthesis.

    Zinc → Rat intestinal metallothioneins measured without isoform resolution source_derived_draftungraded
    Experimental context and source evidence
    cross_nutrient
    Copper (retained_nutrient); Copper bound to rat intestinal metallothioneins (retained_complex)
    evidence_span
    {"source_cache": "artifacts/zinc-signaling-sources/479878-abstract.txt", "locator": "Primary indexed abstract", "file_sha256": "643b0358984f3caac8a20e3202c14a073d11ff88758c7a9637b6f8523195658b"}
    experimental_model
    Dietary radiotracer experiments in rats
    exposure
    High dietary zinc or copper and reciprocal 64Cu/65Zn absorption measurements.
    limitations
    High animal dietary concentrations are not human intake thresholds. Copper displacement from metallothionein was the authors’ mechanistic interpretation.
    nutrient_topic
    Zinc research collection; topical membership is not evidence of a direct dietary effect. · Zinc
    organism
    Rattus norvegicus
    plain_language
    Induced binding proteins provide a mechanism for retaining copper in the gut.
    primary_references
    [zn-sig-479878] Intestinal metallothionein and the mutual antagonism between copper and zinc in the rat. (1979). https://pubmed.ncbi.nlm.nih.gov/479878/ DOI: 10.1016/s0162-0134(00)80054-9
    tissue_or_cell_type
    Small intestine

    Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17) · lines 915–927

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Dietary radiotracer experiments in rats · source_derived_draft · unverified_draft

    ### zn-sig-copper-mt-retention The increased intestinal 64Cu was predominantly metallothionein-associated; the authors inferred copper displacement of zinc after zinc-induced metallothionein synthesis. Condition category: normal nutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect. plain_language: Induced binding proteins provide a mechanism for retaining copper in the gut. organism: Rattus norvegicus tissue_or_cell_type: Small intestine experimental_model: Dietary radiotracer experiments in rats limitations: High animal dietary concentrations are not human intake thresholds. Copper displacement from metallothionein was the authors’ mechanistic interpretation. exposure: High dietary zinc or copper and reciprocal 64Cu/65Zn absorption measurements. cross_nutrient: Copper (retained_nutrient); Copper bound to rat intestinal metallothioneins (retained_complex) evidence_span: {"source_cache": "artifacts/zinc-signaling-sources/479878-abstract.txt", "locator": "Primary indexed abstract", "file_sha256": "643b0358984f3caac8a20e3202c14a073d11ff88758c7a9637b6f8523195658b"} [zn-sig-479878] Intestinal metallothionein and the mutual antagonism between copper and zinc in the rat. (1979). https://pubmed.ncbi.nlm.nih.gov/479878/ DOI: 10.1016/s0162-0134(00)80054-9
    Complete structured claim and evidence

Where it participates (unsigned role)

  1. Raising rat dietary copper from 3 to 24 mg/kg reduced 65Zn absorption by about 20%; further increases to 300 mg/kg did not add inhibition or change zinc association with intestinal metallothionein.

    Copper → Intestinal zinc absorption source_derived_draftungraded
    Experimental context and source evidence
    cross_nutrient
    Zinc (affected_nutrient); Rat intestinal metallothioneins measured without isoform resolution (unchanged_association)
    evidence_span
    {"source_cache": "artifacts/zinc-signaling-sources/479878-abstract.txt", "locator": "Primary indexed abstract", "file_sha256": "643b0358984f3caac8a20e3202c14a073d11ff88758c7a9637b6f8523195658b"}
    experimental_model
    Dietary radiotracer experiments in rats
    exposure
    High dietary zinc or copper and reciprocal 64Cu/65Zn absorption measurements.
    limitations
    High animal dietary concentrations are not human intake thresholds. Copper displacement from metallothionein was the authors’ mechanistic interpretation.
    nutrient_topic
    Zinc research collection; topical membership is not evidence of a direct dietary effect. · Zinc
    organism
    Rattus norvegicus
    plain_language
    Copper also affected zinc absorption, with a different response pattern.
    primary_references
    [zn-sig-479878] Intestinal metallothionein and the mutual antagonism between copper and zinc in the rat. (1979). https://pubmed.ncbi.nlm.nih.gov/479878/ DOI: 10.1016/s0162-0134(00)80054-9
    tissue_or_cell_type
    Small intestine

    Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17) · lines 929–941

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Dietary radiotracer experiments in rats · source_derived_draft · unverified_draft

    ### zn-sig-copper-zinc-absorption Raising rat dietary copper from 3 to 24 mg/kg reduced 65Zn absorption by about 20%; further increases to 300 mg/kg did not add inhibition or change zinc association with intestinal metallothionein. Condition category: normal nutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect. plain_language: Copper also affected zinc absorption, with a different response pattern. organism: Rattus norvegicus tissue_or_cell_type: Small intestine experimental_model: Dietary radiotracer experiments in rats limitations: High animal dietary concentrations are not human intake thresholds. Copper displacement from metallothionein was the authors’ mechanistic interpretation. exposure: High dietary zinc or copper and reciprocal 64Cu/65Zn absorption measurements. cross_nutrient: Zinc (affected_nutrient); Rat intestinal metallothioneins measured without isoform resolution (unchanged_association) evidence_span: {"source_cache": "artifacts/zinc-signaling-sources/479878-abstract.txt", "locator": "Primary indexed abstract", "file_sha256": "643b0358984f3caac8a20e3202c14a073d11ff88758c7a9637b6f8523195658b"} [zn-sig-479878] Intestinal metallothionein and the mutual antagonism between copper and zinc in the rat. (1979). https://pubmed.ncbi.nlm.nih.gov/479878/ DOI: 10.1016/s0162-0134(00)80054-9
    Complete structured claim and evidence

  2. After the tested dietary conditioning, high zinc increased metallothionein-bound copper without changing intracellular copper concentration or significantly altering measured copper absorption in the perfused intestine.

    Zinc → Copper bound to rat intestinal metallothioneins source_derived_draftungraded
    Experimental context and source evidence
    cross_nutrient
    Copper (affected_nutrient); Intestinal copper absorption (unchanged_endpoint); Rat intestinal metallothioneins measured without isoform resolution (binding_proteins)
    evidence_span
    {"source_cache": "artifacts/zinc-signaling-sources/3968585-abstract.txt", "locator": "Primary indexed abstract", "file_sha256": "ca588cec5326d23dd5f8c9b409ea66fc415043b95af456399e495b8131c8f6ed"}
    experimental_model
    Isolated vascularly perfused intestine from diet-conditioned rats
    exposure
    Dietary copper/zinc variation for one week versus direct luminal-metal variation.
    limitations
    Ex-vivo perfusion and extreme luminal exposure differ from whole-animal feeding; increased metallothionein-bound copper alone did not prove reduced net absorption.
    nutrient_topic
    Zinc research collection; topical membership is not evidence of a direct dietary effect. · Zinc
    organism
    Rattus norvegicus
    plain_language
    More copper on a binding protein did not always mean less copper absorption.
    primary_references
    [zn-sig-3968585] Copper and zinc absorption in the rat: mechanism of mutual antagonism. (1985). https://pubmed.ncbi.nlm.nih.gov/3968585/ DOI: 10.1093/jn/115.2.159
    tissue_or_cell_type
    Small-intestinal mucosa and portal effluent

    Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17) · lines 943–955

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Isolated vascularly perfused intestine from diet-conditioned rats · source_derived_draft · unverified_draft

    ### zn-sig-mt-binding-not-absorption After the tested dietary conditioning, high zinc increased metallothionein-bound copper without changing intracellular copper concentration or significantly altering measured copper absorption in the perfused intestine. Condition category: normal nutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect. plain_language: More copper on a binding protein did not always mean less copper absorption. organism: Rattus norvegicus tissue_or_cell_type: Small-intestinal mucosa and portal effluent experimental_model: Isolated vascularly perfused intestine from diet-conditioned rats limitations: Ex-vivo perfusion and extreme luminal exposure differ from whole-animal feeding; increased metallothionein-bound copper alone did not prove reduced net absorption. exposure: Dietary copper/zinc variation for one week versus direct luminal-metal variation. cross_nutrient: Copper (affected_nutrient); Intestinal copper absorption (unchanged_endpoint); Rat intestinal metallothioneins measured without isoform resolution (binding_proteins) evidence_span: {"source_cache": "artifacts/zinc-signaling-sources/3968585-abstract.txt", "locator": "Primary indexed abstract", "file_sha256": "ca588cec5326d23dd5f8c9b409ea66fc415043b95af456399e495b8131c8f6ed"} [zn-sig-3968585] Copper and zinc absorption in the rat: mechanism of mutual antagonism. (1985). https://pubmed.ncbi.nlm.nih.gov/3968585/ DOI: 10.1093/jn/115.2.159
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

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