Component
Peroxisome
Independent organelle record; interpretation is limited by each linked claim and its study context.
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
Human HACL1 expression increased lyase activity in mammalian cells, and GFP fusions showed that its C-terminal sequence could direct peroxisomal localization.
Experimental context and source evidence
- evidence
- [{"paper_key": "foulon-1999-hacl1", "source_bundle": "artifacts/thiamine_metabolism_sources.json", "passage_ids": ["abstract", "p-8"], "locator": "peroxisome targeting signal 1 variant", "preservation": "Exact text retained in the source bundle; full source document retained when openly retrievable."}]
- experimental_model
- Human HACL1 expression and fluorescent localization constructs.
- limitations
- Localization and activity used expression constructs; metal-dependence measurements in this paper were on rat enzyme.
- nutrient
- Thiamine (vitamin B1) · Thiamine (vitamin B1)
- nutrient_topic
- Thiamine research collection; topical membership is not evidence of a direct dietary effect. · Thiamine (vitamin B1)
- organism
- Homo sapiens
- plain_language
- The cloned human protein is an active lyase with a peroxisomal address, distinct from ER-associated HACL2.
- primary_references
- [foulon-1999-hacl1] Purification, molecular cloning, and expression of 2-hydroxyphytanoyl-CoA lyase, a peroxisomal thiamine pyrophosphate-dependent enzyme that catalyzes the carbon-carbon bond cleavage during alpha-oxidation of 3-methyl-branched fatty acids (1999). https://pubmed.ncbi.nlm.nih.gov/10468558/ DOI: 10.1073/pnas.96.18.10039
- tissue_or_cell_type
- Transfected mammalian cells
Thiamine: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 990–1001
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Human HACL1 expression and fluorescent localization constructs. · source_derived_draft · unverified_draft
### b1-hacl1-human-peroxisomal-targeting Human HACL1 expression increased lyase activity in mammalian cells, and GFP fusions showed that its C-terminal sequence could direct peroxisomal localization. Condition category: normal nutrient_topic: Thiamine research collection; topical membership is not evidence of a direct dietary effect. plain_language: The cloned human protein is an active lyase with a peroxisomal address, distinct from ER-associated HACL2. organism: Homo sapiens tissue_or_cell_type: Transfected mammalian cells experimental_model: Human HACL1 expression and fluorescent localization constructs. limitations: Localization and activity used expression constructs; metal-dependence measurements in this paper were on rat enzyme. evidence: [{"paper_key": "foulon-1999-hacl1", "source_bundle": "artifacts/thiamine_metabolism_sources.json", "passage_ids": ["abstract", "p-8"], "locator": "peroxisome targeting signal 1 variant", "preservation": "Exact text retained in the source bundle; full source document retained when openly retrievable."}] nutrient: Thiamine (vitamin B1) [foulon-1999-hacl1] Purification, molecular cloning, and expression of 2-hydroxyphytanoyl-CoA lyase, a peroxisomal thiamine pyrophosphate-dependent enzyme that catalyzes the carbon-carbon bond cleavage during alpha-oxidation of 3-methyl-branched fatty acids (1999). https://pubmed.ncbi.nlm.nih.gov/10468558/ DOI: 10.1073/pnas.96.18.10039
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.