Component
Human kidney NBCe1 E91R/R298E charge-reversal construct
Human kidney NBCe1 E91R/R298E charge-reversal construct. Species, exposure and limitations are retained in each linked claim.
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What it acts on
The E91R/R298E charge-reversal construct restored transport function in the study.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- evidence_span
- {"source_cache": "artifacts/sodium-research/18441326.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "3ca538a7ecb046a47f9246778b3dede99c503a45c6048f75541612c8db37448c", "start_char": 0, "end_char": 1213, "text_sha256": "3ca538a7ecb046a47f9246778b3dede99c503a45c6048f75541612c8db37448c"}
- experimental_model
- Homology modeling and site-directed mutagenesis with oocyte transport assays
- exposure
- E91 and R298 mutations and charge-reversal construct
- limitations
- Structure model plus functional assay; charge rescue does not establish clinical treatment or dietary sodium responsiveness.
- nutrient_topic
- Sodium research collection; topical membership is not evidence of a direct dietary effect. · Sodium
- organism
- Human kidney NBCe1 in Xenopus oocytes
- plain_language
- An engineered protein repair supported a specific interaction between these residues.
- primary_references
- [sodium-p18441326] Entry to "formula tunnel" revealed by SLC4A4 human mutation and structural model. (2008). https://pubmed.ncbi.nlm.nih.gov/18441326/ DOI: 10.1074/jbc.m709819200
- tissue_or_cell_type
- Sodium/bicarbonate transporter N-terminal domain
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Sodium: gradients, nutrient transport, fluid regulation and loss states (2026-09-17) · lines 720–731
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Homology modeling and site-directed mutagenesis with oocyte transport assays · source_derived_draft · unverified_draft
### sodium-nbce1-rescue The E91R/R298E charge-reversal construct restored transport function in the study. Condition category: machinery_impairment nutrient_topic: Sodium research collection; topical membership is not evidence of a direct dietary effect. plain_language: An engineered protein repair supported a specific interaction between these residues. organism: Human kidney NBCe1 in Xenopus oocytes tissue_or_cell_type: Sodium/bicarbonate transporter N-terminal domain experimental_model: Homology modeling and site-directed mutagenesis with oocyte transport assays limitations: Structure model plus functional assay; charge rescue does not establish clinical treatment or dietary sodium responsiveness. exposure: E91 and R298 mutations and charge-reversal construct evidence_span: {"source_cache": "artifacts/sodium-research/18441326.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "3ca538a7ecb046a47f9246778b3dede99c503a45c6048f75541612c8db37448c", "start_char": 0, "end_char": 1213, "text_sha256": "3ca538a7ecb046a47f9246778b3dede99c503a45c6048f75541612c8db37448c"} [sodium-p18441326] Entry to "formula tunnel" revealed by SLC4A4 human mutation and structural model. (2008). https://pubmed.ncbi.nlm.nih.gov/18441326/ DOI: 10.1074/jbc.m709819200
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.