Component

Mouse vascular smooth-muscle Slc20a1 knockout

Mouse vascular smooth-muscle Slc20a1 knockout. Species, exposure and limitations are retained in each linked claim.

4 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What it acts on

  1. PiT1 deletion did not reduce aortic calcification in the mouse CKD/phosphate-loading model.

    Mouse vascular smooth-muscle Slc20a1 knockout → Mouse vascular smooth-muscle matrix mineralization source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    machinery_impairment Imported condition classification; unverified.
    evidence_span
    {"source_cache": "artifacts/phosphorus-research/23968976.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "6704ac0400a286d5137384f4339dd6dffb0061a41a121b6c72a0b23353cbc6f9", "start_char": 0, "end_char": 1852, "text_sha256": "6704ac0400a286d5137384f4339dd6dffb0061a41a121b6c72a0b23353cbc6f9"}
    experimental_model
    Smooth-muscle-specific knockout and transporter compensation experiments
    exposure
    Mouse CKD plus dietary phosphate loading; PiT2 knockdown or expression rescue
    limitations
    Loss of one transporter can be masked by another. This refines a single-transporter model without proving every calcification pathway requires transport.
    nutrient_topic
    Phosphorus research collection; topical membership is not evidence of a direct dietary effect. · Phosphorus
    organism
    Mouse
    plain_language
    Removing PiT1 alone did not protect these mice from calcification.
    primary_references
    [phosphorus-p23968976] Sodium-dependent phosphate cotransporters and phosphate-induced calcification of vascular smooth muscle cells: redundant roles for PiT-1 and PiT-2. (2013). https://pubmed.ncbi.nlm.nih.gov/23968976/ DOI: 10.1161/atvbaha.113.302249
    tissue_or_cell_type
    Vascular smooth muscle
    trigger_kind
    machinery_impairment Imported condition classification; unverified.

    Phosphorus: metabolism, signaling and nutrient connections (2026-09-17) · lines 815–826

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Smooth-muscle-specific knockout and transporter compensation experiments · source_derived_draft · unverified_draft

    ### phosphorus-pit1-null-calcification PiT1 deletion did not reduce aortic calcification in the mouse CKD/phosphate-loading model. Condition category: machinery_impairment nutrient_topic: Phosphorus research collection; topical membership is not evidence of a direct dietary effect. plain_language: Removing PiT1 alone did not protect these mice from calcification. organism: Mouse tissue_or_cell_type: Vascular smooth muscle experimental_model: Smooth-muscle-specific knockout and transporter compensation experiments limitations: Loss of one transporter can be masked by another. This refines a single-transporter model without proving every calcification pathway requires transport. exposure: Mouse CKD plus dietary phosphate loading; PiT2 knockdown or expression rescue evidence_span: {"source_cache": "artifacts/phosphorus-research/23968976.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "6704ac0400a286d5137384f4339dd6dffb0061a41a121b6c72a0b23353cbc6f9", "start_char": 0, "end_char": 1852, "text_sha256": "6704ac0400a286d5137384f4339dd6dffb0061a41a121b6c72a0b23353cbc6f9"} [phosphorus-p23968976] Sodium-dependent phosphate cotransporters and phosphate-induced calcification of vascular smooth muscle cells: redundant roles for PiT-1 and PiT-2. (2013). https://pubmed.ncbi.nlm.nih.gov/23968976/ DOI: 10.1161/atvbaha.113.302249
    Complete structured claim and evidence

  2. Aortic medial PiT2 mRNA increased approximately twofold after smooth-muscle PiT1 deletion.

    Mouse vascular smooth-muscle Slc20a1 knockout → Mouse phosphate transporter PiT-2 / Slc20a2 source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    machinery_impairment Imported condition classification; unverified.
    evidence_span
    {"source_cache": "artifacts/phosphorus-research/23968976.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "6704ac0400a286d5137384f4339dd6dffb0061a41a121b6c72a0b23353cbc6f9", "start_char": 0, "end_char": 1852, "text_sha256": "6704ac0400a286d5137384f4339dd6dffb0061a41a121b6c72a0b23353cbc6f9"}
    experimental_model
    Smooth-muscle-specific knockout and transporter compensation experiments
    exposure
    Mouse CKD plus dietary phosphate loading; PiT2 knockdown or expression rescue
    limitations
    Loss of one transporter can be masked by another. This refines a single-transporter model without proving every calcification pathway requires transport.
    nutrient_topic
    Phosphorus research collection; topical membership is not evidence of a direct dietary effect. · Phosphorus
    organism
    Mouse
    plain_language
    The backup transporter increased when the first transporter was absent.
    primary_references
    [phosphorus-p23968976] Sodium-dependent phosphate cotransporters and phosphate-induced calcification of vascular smooth muscle cells: redundant roles for PiT-1 and PiT-2. (2013). https://pubmed.ncbi.nlm.nih.gov/23968976/ DOI: 10.1161/atvbaha.113.302249
    tissue_or_cell_type
    Vascular smooth muscle
    trigger_kind
    machinery_impairment Imported condition classification; unverified.

    Phosphorus: metabolism, signaling and nutrient connections (2026-09-17) · lines 802–813

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Smooth-muscle-specific knockout and transporter compensation experiments · source_derived_draft · unverified_draft

    ### phosphorus-pit2-compensation Aortic medial PiT2 mRNA increased approximately twofold after smooth-muscle PiT1 deletion. Condition category: machinery_impairment nutrient_topic: Phosphorus research collection; topical membership is not evidence of a direct dietary effect. plain_language: The backup transporter increased when the first transporter was absent. organism: Mouse tissue_or_cell_type: Vascular smooth muscle experimental_model: Smooth-muscle-specific knockout and transporter compensation experiments limitations: Loss of one transporter can be masked by another. This refines a single-transporter model without proving every calcification pathway requires transport. exposure: Mouse CKD plus dietary phosphate loading; PiT2 knockdown or expression rescue evidence_span: {"source_cache": "artifacts/phosphorus-research/23968976.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "6704ac0400a286d5137384f4339dd6dffb0061a41a121b6c72a0b23353cbc6f9", "start_char": 0, "end_char": 1852, "text_sha256": "6704ac0400a286d5137384f4339dd6dffb0061a41a121b6c72a0b23353cbc6f9"} [phosphorus-p23968976] Sodium-dependent phosphate cotransporters and phosphate-induced calcification of vascular smooth muscle cells: redundant roles for PiT-1 and PiT-2. (2013). https://pubmed.ncbi.nlm.nih.gov/23968976/ DOI: 10.1161/atvbaha.113.302249
    Complete structured claim and evidence

Where it participates (unsigned role)

  1. PiT2 knockdown reduced phosphate-induced calcification in PiT1-deficient mouse vascular smooth-muscle cells.

    Experimental mouse Slc20a2 knockdown → Mouse vascular smooth-muscle matrix mineralization source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    machinery_impairment Imported condition classification; unverified.
    evidence_span
    {"source_cache": "artifacts/phosphorus-research/23968976.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "6704ac0400a286d5137384f4339dd6dffb0061a41a121b6c72a0b23353cbc6f9", "start_char": 0, "end_char": 1852, "text_sha256": "6704ac0400a286d5137384f4339dd6dffb0061a41a121b6c72a0b23353cbc6f9"}
    experimental_model
    Smooth-muscle-specific knockout and transporter compensation experiments
    exposure
    Mouse CKD plus dietary phosphate loading; PiT2 knockdown or expression rescue
    limitations
    Loss of one transporter can be masked by another. This refines a single-transporter model without proving every calcification pathway requires transport.
    nutrient_topic
    Phosphorus research collection; topical membership is not evidence of a direct dietary effect. · Phosphorus
    organism
    Mouse
    plain_language
    Removing the backup also reduced mineral deposition.
    primary_references
    [phosphorus-p23968976] Sodium-dependent phosphate cotransporters and phosphate-induced calcification of vascular smooth muscle cells: redundant roles for PiT-1 and PiT-2. (2013). https://pubmed.ncbi.nlm.nih.gov/23968976/ DOI: 10.1161/atvbaha.113.302249
    tissue_or_cell_type
    Vascular smooth muscle
    trigger_kind
    machinery_impairment Imported condition classification; unverified.

    Phosphorus: metabolism, signaling and nutrient connections (2026-09-17) · lines 841–852

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Smooth-muscle-specific knockout and transporter compensation experiments · source_derived_draft · unverified_draft

    ### phosphorus-pit2-knockdown-mineral PiT2 knockdown reduced phosphate-induced calcification in PiT1-deficient mouse vascular smooth-muscle cells. Condition category: machinery_impairment nutrient_topic: Phosphorus research collection; topical membership is not evidence of a direct dietary effect. plain_language: Removing the backup also reduced mineral deposition. organism: Mouse tissue_or_cell_type: Vascular smooth muscle experimental_model: Smooth-muscle-specific knockout and transporter compensation experiments limitations: Loss of one transporter can be masked by another. This refines a single-transporter model without proving every calcification pathway requires transport. exposure: Mouse CKD plus dietary phosphate loading; PiT2 knockdown or expression rescue evidence_span: {"source_cache": "artifacts/phosphorus-research/23968976.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "6704ac0400a286d5137384f4339dd6dffb0061a41a121b6c72a0b23353cbc6f9", "start_char": 0, "end_char": 1852, "text_sha256": "6704ac0400a286d5137384f4339dd6dffb0061a41a121b6c72a0b23353cbc6f9"} [phosphorus-p23968976] Sodium-dependent phosphate cotransporters and phosphate-induced calcification of vascular smooth muscle cells: redundant roles for PiT-1 and PiT-2. (2013). https://pubmed.ncbi.nlm.nih.gov/23968976/ DOI: 10.1161/atvbaha.113.302249
    Complete structured claim and evidence

  2. PiT2 knockdown reduced phosphate uptake in PiT1-deficient mouse vascular smooth-muscle cells.

    Experimental mouse Slc20a2 knockdown → Mouse vascular smooth-muscle phosphate uptake source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    machinery_impairment Imported condition classification; unverified.
    evidence_span
    {"source_cache": "artifacts/phosphorus-research/23968976.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "6704ac0400a286d5137384f4339dd6dffb0061a41a121b6c72a0b23353cbc6f9", "start_char": 0, "end_char": 1852, "text_sha256": "6704ac0400a286d5137384f4339dd6dffb0061a41a121b6c72a0b23353cbc6f9"}
    experimental_model
    Smooth-muscle-specific knockout and transporter compensation experiments
    exposure
    Mouse CKD plus dietary phosphate loading; PiT2 knockdown or expression rescue
    limitations
    Loss of one transporter can be masked by another. This refines a single-transporter model without proving every calcification pathway requires transport.
    nutrient_topic
    Phosphorus research collection; topical membership is not evidence of a direct dietary effect. · Phosphorus
    organism
    Mouse
    plain_language
    Removing the backup reduced uptake.
    primary_references
    [phosphorus-p23968976] Sodium-dependent phosphate cotransporters and phosphate-induced calcification of vascular smooth muscle cells: redundant roles for PiT-1 and PiT-2. (2013). https://pubmed.ncbi.nlm.nih.gov/23968976/ DOI: 10.1161/atvbaha.113.302249
    tissue_or_cell_type
    Vascular smooth muscle
    trigger_kind
    machinery_impairment Imported condition classification; unverified.

    Phosphorus: metabolism, signaling and nutrient connections (2026-09-17) · lines 828–839

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Smooth-muscle-specific knockout and transporter compensation experiments · source_derived_draft · unverified_draft

    ### phosphorus-pit2-knockdown-uptake PiT2 knockdown reduced phosphate uptake in PiT1-deficient mouse vascular smooth-muscle cells. Condition category: machinery_impairment nutrient_topic: Phosphorus research collection; topical membership is not evidence of a direct dietary effect. plain_language: Removing the backup reduced uptake. organism: Mouse tissue_or_cell_type: Vascular smooth muscle experimental_model: Smooth-muscle-specific knockout and transporter compensation experiments limitations: Loss of one transporter can be masked by another. This refines a single-transporter model without proving every calcification pathway requires transport. exposure: Mouse CKD plus dietary phosphate loading; PiT2 knockdown or expression rescue evidence_span: {"source_cache": "artifacts/phosphorus-research/23968976.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "6704ac0400a286d5137384f4339dd6dffb0061a41a121b6c72a0b23353cbc6f9", "start_char": 0, "end_char": 1852, "text_sha256": "6704ac0400a286d5137384f4339dd6dffb0061a41a121b6c72a0b23353cbc6f9"} [phosphorus-p23968976] Sodium-dependent phosphate cotransporters and phosphate-induced calcification of vascular smooth muscle cells: redundant roles for PiT-1 and PiT-2. (2013). https://pubmed.ncbi.nlm.nih.gov/23968976/ DOI: 10.1161/atvbaha.113.302249
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

    Evidence, AI assistance and curation standards