Component
Modafinil affinity for human DAT in binding assays
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
Adding 10 micromolar zinc in sodium-free binding buffer did not significantly improve modafinil affinity for DAT: Ki approximately 2.96 versus 3.47 micromolar.
Experimental context and source evidence
- evidence_access
- Full XML obtained; relevant results/methods inspected. Not exhaustive reanalysis of figures or data.
- experimental_condition
- Same sodium-free buffer without added zinc 10 micromolar added zinc · Zinc(II) ion Condition belongs to the full experimental contrast; do not separate a joint intervention.
- experimental_contrast
- {"intervention": "10 micromolar zinc in sodium-free buffer", "comparator": "Same sodium-free buffer without added zinc", "endpoint": "Adding 10 micromolar zinc in sodium-free binding buffer did not significantly improve modafinil affinity for DAT: Ki approximately 2.96 versus 3.47 micromolar.", "effect_direction": "no_detected_change", "combination": "joint", "conditions": [{"entity_slug": "zinc-ion", "state": "10 micromolar added zinc"}, {"entity_slug": null, "state": "sodium-free buffer"}]} Explicit extracted experimental comparison; source-derived draft.
- experimental_model
- Human DAT binding assay, sodium-free buffer +/- 10 micromolar zinc; not dietary sodium deficiency or a supplement interaction.
- interpretation_status
- Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.
- limitations
- Interpret only within the recorded preparation, exposure and comparator. The complete source passage retains qualifications; unspecified doses/timing have not been extracted here. No clinical efficacy, nutrient deficiency or unique molecular mediation is inferred.
- plain_language
- Adding 10 micromolar zinc in sodium-free binding buffer did not significantly improve modafinil affinity for DAT: Ki approximately 2.96 versus 3.47 micromolar.
- primary_references
- The atypical stimulant and nootropic modafinil interacts with the dopamine transporter in a different manner than classical cocaine-like inhibitors. | 2011 | DOI 10.1371/journal.pone.0025790 | PMID 22043293 | https://pmc.ncbi.nlm.nih.gov/articles/PMC3197159/ | https://pubmed.ncbi.nlm.nih.gov/22043293/
- source_locator
- Reviewed reference lines 56-56; exact primary location described in quoted passage where extracted.
Modafinil: detailed mechanisms of action (reviewed 3 October 2026) · lines 56–56
Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication. · supports · Human DAT binding assay, sodium-free buffer +/- 10 micromolar zinc; not dietary sodium deficiency or a supplement interaction. · Recorded from source · Recorded from source
A useful **direct nutrient–drug comparison** is contained in that study: in sodium-free binding buffer, adding **10 µM zinc** increased cocaine/methylphenidate binding affinity, but did not significantly improve modafinil affinity. Modafinil Kᵢ was approximately 2.96 µM without zinc and 3.47 µM with it. This is a combined ion-condition experiment, not proof that dietary zinc enhances modafinil or that zinc never affects its response in vivo. Sodium removal is also an assay manipulation, not a model of ordinary dietary sodium deficiency. [Table 2 and methods](https://pmc.ncbi.nlm.nih.gov/articles/PMC3197159/)
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.