Component
Whole-cell methylmalonyl-CoA pool
Whole-cell methylmalonyl-CoA pool
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
Adding 2.5 micromolar B12 during ECHDC1-knockout L929 adipocyte differentiation lowered whole-cell methylmalonyl-CoA approximately 20-fold.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- cross_nutrient
- false
- evidence_location
- Full text Results: vitamin B12 supplementation; Figure 4g-u
- experimental_model
- Differentiating ECHDC1-knockout mouse L929 adipocytes (Figure 4g-u)
- exposure
- ECHDC1 knockout; 2.5 micromolar supplementary B12 during differentiation
- limitations
- Whole-cell LC-MS pools; no compartment or epimer resolution and no direct pathway flux measurement.
- nutrient_topic
- Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect. · Vitamin B12 (cobalamins)
- organism
- Mus musculus
- plain_language
- B12 addition sharply lowered this measured precursor pool in cultured mouse adipocytes. The cells had ECHDC1 deleted.
- primary_references
- [dewulf-2019-l929-b12] The synthesis of branched-chain fatty acids is limited by enzymatic decarboxylation of ethyl- and methylmalonyl-CoA. (2019). https://pubmed.ncbi.nlm.nih.gov/31416829/ DOI: 10.1042/bcj20190500
- tissue_or_cell_type
- Adipocytes
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Vitamin B12: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 1258–1270
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Differentiating ECHDC1-knockout mouse L929 adipocytes (Figure 4g-u) · source_derived_draft · unverified_draft
### l929-b12-lowers-methylmalonyl-pool Adding 2.5 micromolar B12 during ECHDC1-knockout L929 adipocyte differentiation lowered whole-cell methylmalonyl-CoA approximately 20-fold. Condition category: machinery_impairment nutrient_topic: Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect. plain_language: B12 addition sharply lowered this measured precursor pool in cultured mouse adipocytes. The cells had ECHDC1 deleted. organism: Mus musculus tissue_or_cell_type: Adipocytes experimental_model: Differentiating ECHDC1-knockout mouse L929 adipocytes (Figure 4g-u) limitations: Whole-cell LC-MS pools; no compartment or epimer resolution and no direct pathway flux measurement. exposure: ECHDC1 knockout; 2.5 micromolar supplementary B12 during differentiation cross_nutrient: false evidence_location: Full text Results: vitamin B12 supplementation; Figure 4g-u [dewulf-2019-l929-b12] The synthesis of branched-chain fatty acids is limited by enzymatic decarboxylation of ethyl- and methylmalonyl-CoA. (2019). https://pubmed.ncbi.nlm.nih.gov/31416829/ DOI: 10.1042/bcj20190500
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.