Component

Lymphoma cell death

Cell-death endpoint in lymphoma cultures; assay and line specified per claim.

3 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What acts on it

  1. Extracellular catalase prevented JLP-119 cell death after 2 mM ascorbate, supporting peroxide as a required mediator in this culture experiment.

    Experimental catalase preparation → Lymphoma cell death source_derived_draftungraded
    Experimental context and source evidence
    cross_nutrient
    false
    evidence_location
    Figure 3A
    experimental_model
    Human JLP-119 Burkitt lymphoma culture; 1-h exposure, washing, Hoechst/propidium iodide cell-death assessment after 18–22 h
    exposure
    Fresh L-ascorbic acid neutralized to pH 7.0; 2 mM extracellular ascorbate for 1 h; cells 2.5 × 10^5/mL in serum-containing culture medium. Catalase 100 µg/mL preincubated 30 min.
    limitations
    Primary paper identifies catalase but does not specify species in the inspected Methods; the source species should not be inferred. Pharmacological in-vitro exposure; medium chemistry and peroxide clearance differ from intact tissues. No dietary or clinical anticancer inference.
    nutrient_topic
    Vitamin C research collection; topical membership is not evidence of a direct dietary effect. · Vitamin C
    organism
    Homo sapiens cells; experimental catalase preparation
    plain_language
    Breaking down peroxide outside the cells prevented the vitamin C-associated killing.
    primary_references
    [c-reg-chen] Pharmacologic ascorbic acid concentrations selectively kill cancer cells: action as a pro-drug to deliver hydrogen peroxide to tissues. (2005). https://pubmed.ncbi.nlm.nih.gov/16157892/ DOI: 10.1073/pnas.0506390102
    tissue_or_cell_type
    Burkitt lymphoma cells

    Vitamin C: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 1243–1255

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Human JLP-119 Burkitt lymphoma culture; 1-h exposure, washing, Hoechst/propidium iodide cell-death assessment after 18–22 h · source_derived_draft · unverified_draft

    ### c-reg-catalase-protects-lymphoma Extracellular catalase prevented JLP-119 cell death after 2 mM ascorbate, supporting peroxide as a required mediator in this culture experiment. Condition category: normal nutrient_topic: Vitamin C research collection; topical membership is not evidence of a direct dietary effect. plain_language: Breaking down peroxide outside the cells prevented the vitamin C-associated killing. organism: Homo sapiens cells; experimental catalase preparation tissue_or_cell_type: Burkitt lymphoma cells experimental_model: Human JLP-119 Burkitt lymphoma culture; 1-h exposure, washing, Hoechst/propidium iodide cell-death assessment after 18–22 h limitations: Primary paper identifies catalase but does not specify species in the inspected Methods; the source species should not be inferred. Pharmacological in-vitro exposure; medium chemistry and peroxide clearance differ from intact tissues. No dietary or clinical anticancer inference. exposure: Fresh L-ascorbic acid neutralized to pH 7.0; 2 mM extracellular ascorbate for 1 h; cells 2.5 × 10^5/mL in serum-containing culture medium. Catalase 100 µg/mL preincubated 30 min. cross_nutrient: false evidence_location: Figure 3A [c-reg-chen] Pharmacologic ascorbic acid concentrations selectively kill cancer cells: action as a pro-drug to deliver hydrogen peroxide to tissues. (2005). https://pubmed.ncbi.nlm.nih.gov/16157892/ DOI: 10.1073/pnas.0506390102
    Complete structured claim and evidence

  2. Adding human erythrocytes at 25% or 50% hematocrit protected JLP-119 lymphoma cells from the 2 mM ascorbate challenge, demonstrating that blood-cell clearance can change an otherwise cytotoxic culture exposure.

    Erythrocyte peroxide scavenging → Lymphoma cell death source_derived_draftungraded
    Experimental context and source evidence
    cross_nutrient
    false
    evidence_location
    Figure 5C; Methods RBC mixtures
    experimental_model
    Human JLP-119 Burkitt lymphoma culture; 1-h exposure, washing, Hoechst/propidium iodide cell-death assessment after 18–22 h with added human red blood cells
    exposure
    Fresh L-ascorbic acid neutralized to pH 7.0; 2 mM extracellular ascorbate for 1 h; cells 2.5 × 10^5/mL in serum-containing culture medium. Reconstituted 25% or 50% hematocrit; lymphoma cells recovered before later assessment.
    limitations
    Pharmacological in-vitro exposure; medium chemistry and peroxide clearance differ from intact tissues. No dietary or clinical anticancer inference. Protective blood-cell mixture is not proof that all extravascular compartments are equally protected.
    nutrient_topic
    Vitamin C research collection; topical membership is not evidence of a direct dietary effect. · Vitamin C
    organism
    Homo sapiens
    plain_language
    Red blood cells removed the damaging chemistry sufficiently to protect the lymphoma cells in this mixture.
    primary_references
    [c-reg-chen] Pharmacologic ascorbic acid concentrations selectively kill cancer cells: action as a pro-drug to deliver hydrogen peroxide to tissues. (2005). https://pubmed.ncbi.nlm.nih.gov/16157892/ DOI: 10.1073/pnas.0506390102
    tissue_or_cell_type
    Lymphoma/erythrocyte coculture

    Vitamin C: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 1271–1283

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Human JLP-119 Burkitt lymphoma culture; 1-h exposure, washing, Hoechst/propidium iodide cell-death assessment after 18–22 h with added human red blood cells · source_derived_draft · unverified_draft

    ### c-reg-erythrocytes-protect-lymphoma Adding human erythrocytes at 25% or 50% hematocrit protected JLP-119 lymphoma cells from the 2 mM ascorbate challenge, demonstrating that blood-cell clearance can change an otherwise cytotoxic culture exposure. Condition category: normal nutrient_topic: Vitamin C research collection; topical membership is not evidence of a direct dietary effect. plain_language: Red blood cells removed the damaging chemistry sufficiently to protect the lymphoma cells in this mixture. organism: Homo sapiens tissue_or_cell_type: Lymphoma/erythrocyte coculture experimental_model: Human JLP-119 Burkitt lymphoma culture; 1-h exposure, washing, Hoechst/propidium iodide cell-death assessment after 18–22 h with added human red blood cells limitations: Pharmacological in-vitro exposure; medium chemistry and peroxide clearance differ from intact tissues. No dietary or clinical anticancer inference. Protective blood-cell mixture is not proof that all extravascular compartments are equally protected. exposure: Fresh L-ascorbic acid neutralized to pH 7.0; 2 mM extracellular ascorbate for 1 h; cells 2.5 × 10^5/mL in serum-containing culture medium. Reconstituted 25% or 50% hematocrit; lymphoma cells recovered before later assessment. cross_nutrient: false evidence_location: Figure 5C; Methods RBC mixtures [c-reg-chen] Pharmacologic ascorbic acid concentrations selectively kill cancer cells: action as a pro-drug to deliver hydrogen peroxide to tissues. (2005). https://pubmed.ncbi.nlm.nih.gov/16157892/ DOI: 10.1073/pnas.0506390102
    Complete structured claim and evidence

  3. JLP-119 lymphoma cells died after extracellular ascorbate exposure even when previously loaded to about 3 mM intracellular ascorbate; matched loading through dehydroascorbic acid did not reproduce the extracellular-ascorbate death effect.

    L-Ascorbate → Lymphoma cell death source_derived_draftungraded
    Experimental context and source evidence
    cross_nutrient
    false
    evidence_location
    Figure 2D–E
    experimental_model
    Human JLP-119 Burkitt lymphoma culture; 1-h exposure, washing, Hoechst/propidium iodide cell-death assessment after 18–22 h
    exposure
    Fresh L-ascorbic acid neutralized to pH 7.0; 2 mM extracellular ascorbate for 1 h; cells 2.5 × 10^5/mL in serum-containing culture medium.
    limitations
    Pharmacological in-vitro exposure; medium chemistry and peroxide clearance differ from intact tissues. No dietary or clinical anticancer inference. This conclusion is specific to this line and loading protocol.
    nutrient_topic
    Vitamin C research collection; topical membership is not evidence of a direct dietary effect. · Vitamin C
    organism
    Homo sapiens
    plain_language
    The killing depended on vitamin C outside these lymphoma cells, not merely on having vitamin C inside them.
    primary_references
    [c-reg-chen] Pharmacologic ascorbic acid concentrations selectively kill cancer cells: action as a pro-drug to deliver hydrogen peroxide to tissues. (2005). https://pubmed.ncbi.nlm.nih.gov/16157892/ DOI: 10.1073/pnas.0506390102
    tissue_or_cell_type
    Burkitt lymphoma cells

    Vitamin C: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 1229–1241

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Human JLP-119 Burkitt lymphoma culture; 1-h exposure, washing, Hoechst/propidium iodide cell-death assessment after 18–22 h · source_derived_draft · unverified_draft

    ### c-reg-lymphoma-extracellular-ascorbate JLP-119 lymphoma cells died after extracellular ascorbate exposure even when previously loaded to about 3 mM intracellular ascorbate; matched loading through dehydroascorbic acid did not reproduce the extracellular-ascorbate death effect. Condition category: normal nutrient_topic: Vitamin C research collection; topical membership is not evidence of a direct dietary effect. plain_language: The killing depended on vitamin C outside these lymphoma cells, not merely on having vitamin C inside them. organism: Homo sapiens tissue_or_cell_type: Burkitt lymphoma cells experimental_model: Human JLP-119 Burkitt lymphoma culture; 1-h exposure, washing, Hoechst/propidium iodide cell-death assessment after 18–22 h limitations: Pharmacological in-vitro exposure; medium chemistry and peroxide clearance differ from intact tissues. No dietary or clinical anticancer inference. This conclusion is specific to this line and loading protocol. exposure: Fresh L-ascorbic acid neutralized to pH 7.0; 2 mM extracellular ascorbate for 1 h; cells 2.5 × 10^5/mL in serum-containing culture medium. cross_nutrient: false evidence_location: Figure 2D–E [c-reg-chen] Pharmacologic ascorbic acid concentrations selectively kill cancer cells: action as a pro-drug to deliver hydrogen peroxide to tissues. (2005). https://pubmed.ncbi.nlm.nih.gov/16157892/ DOI: 10.1073/pnas.0506390102
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

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