Component
Iodoacetamide-containing KEAP1 mass-spectrometry sample preparation
Iodoacetamide-containing KEAP1 mass-spectrometry sample preparation. Species, exposure and limitations are retained in each linked claim.
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
Iodoacetamide-based sample preparation caused loss of reversible sulforaphane adducts at reactive KEAP1 cysteines including C151.
Experimental context and source evidence
- evidence_span
- {"source_cache": "artifacts/sulforaphane-research/21391649.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "d1ecbe68352504813e0a666dc7474fba7f0a3f3aeb5d0b2fea681d376df999ac", "start_char": 0, "end_char": 1511, "text_sha256": "d1ecbe68352504813e0a666dc7474fba7f0a3f3aeb5d0b2fea681d376df999ac"}
- experimental_model
- Mass spectrometry with revised sample preparation
- exposure
- Sulforaphane exposure; omission of iodoacetamide and shorter processing
- limitations
- Explains an assay-dependent detection problem; not an unresolved universal C151 disagreement or a human efficacy trial.
- nutrient_topic
- Sulforaphane research collection; topical membership is not evidence of a direct dietary effect. · Sulforaphane / SFN, stereochemistry specified per study
- organism
- KEAP1 protein cysteine mapping
- plain_language
- An apparent missing binding site was explained by how the sample was prepared.
- primary_references
- [sulforaphane-p21391649] Modification of keap1 cysteine residues by sulforaphane. (2011). https://pubmed.ncbi.nlm.nih.gov/21391649/ DOI: 10.1021/tx100389r
- tissue_or_cell_type
- Reversible electrophile-thiol adducts
Sulforaphane: formation, electrophile sensing and nutrient connections (2026-09-17) · lines 437–448
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Mass spectrometry with revised sample preparation · source_derived_draft · unverified_draft
### sulforaphane-adduct-loss-in-assay Iodoacetamide-based sample preparation caused loss of reversible sulforaphane adducts at reactive KEAP1 cysteines including C151. Condition category: normal nutrient_topic: Sulforaphane research collection; topical membership is not evidence of a direct dietary effect. plain_language: An apparent missing binding site was explained by how the sample was prepared. organism: KEAP1 protein cysteine mapping tissue_or_cell_type: Reversible electrophile-thiol adducts experimental_model: Mass spectrometry with revised sample preparation limitations: Explains an assay-dependent detection problem; not an unresolved universal C151 disagreement or a human efficacy trial. exposure: Sulforaphane exposure; omission of iodoacetamide and shorter processing evidence_span: {"source_cache": "artifacts/sulforaphane-research/21391649.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "d1ecbe68352504813e0a666dc7474fba7f0a3f3aeb5d0b2fea681d376df999ac", "start_char": 0, "end_char": 1511, "text_sha256": "d1ecbe68352504813e0a666dc7474fba7f0a3f3aeb5d0b2fea681d376df999ac"} [sulforaphane-p21391649] Modification of keap1 cysteine residues by sulforaphane. (2011). https://pubmed.ncbi.nlm.nih.gov/21391649/ DOI: 10.1021/tx100389r
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.