Component

Iron reduction in cell-free chemical assays

Iron reduction in cell-free chemical assays. Interpret through the linked experimental species, preparation, compartment and exposure; no universal causal effect is implied.

2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What acts on it

  1. Purified cyanidin-3-glucoside reduced iron in the reported cell-free assays.

    Cyanidin 3-O-beta-D-glucopyranoside → Iron reduction in cell-free chemical assays source_derived_draftungraded
    Experimental context and source evidence
    evidence_access
    Primary article abstract read in indexed publisher/author-hosted result; publisher full text unavailable. Exact pH series and extract/metal ratios not extracted.
    experimental_model
    Cell-free spectrophotometric metal assays; multiple pH and compound/metal ratios; exact series not extracted.
    interpretation_status
    Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.
    limitations
    Binding or reduction, not human absorption, intracellular stripping or ferroptosis prevention. Exact assay metal pools and doses require full methods.
    plain_language
    Purified cyanidin-3-glucoside reduced iron in the reported cell-free assays.
    primary_references
    Fruit extracts of 10 varieties of elderberry (Sambucus nigra L.) interact differently with iron and copper | 2016 | DOI 10.1016/j.phytol.2016.10.025 | https://doi.org/10.1016/j.phytol.2016.10.025
    source_locator
    Reviewed reference lines 88-88; exact primary location described in quoted passage where extracted.

    Anthocyanins: detailed mechanisms of action (reviewed 4 October 2026) · lines 88–88

    Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication. · supports · Cell-free spectrophotometric metal assays; multiple pH and compound/metal ratios; exact series not extracted. · source_derived_draft · unverified_draft

    **Iron binding and metal reduction are different reactions.** Purified cyanidin, C3G and standardized elderberry extracts chelated iron and reduced iron and copper in cell-free assays; copper-chelating activity was relatively low. Extract behavior varied and was not completely explained by pigment concentration. Binding a metal, changing its oxidation state and reducing intestinal absorption are three different endpoints. This does not establish removal of intracellular metals or protection from ferroptosis in people. [Mladěnka et al., 2016](https://doi.org/10.1016/j.phytol.2016.10.025).
    Complete structured claim and evidence

  2. Purified cyanidin reduced iron in the reported cell-free assays.

    Cyanidin → Iron reduction in cell-free chemical assays source_derived_draftungraded
    Experimental context and source evidence
    evidence_access
    Primary article abstract read in indexed publisher/author-hosted result; publisher full text unavailable. Exact pH series and extract/metal ratios not extracted.
    experimental_model
    Cell-free spectrophotometric metal assays; multiple pH and compound/metal ratios; exact series not extracted.
    interpretation_status
    Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.
    limitations
    Binding or reduction, not human absorption, intracellular stripping or ferroptosis prevention. Exact assay metal pools and doses require full methods.
    plain_language
    Purified cyanidin reduced iron in the reported cell-free assays.
    primary_references
    Fruit extracts of 10 varieties of elderberry (Sambucus nigra L.) interact differently with iron and copper | 2016 | DOI 10.1016/j.phytol.2016.10.025 | https://doi.org/10.1016/j.phytol.2016.10.025
    source_locator
    Reviewed reference lines 88-88; exact primary location described in quoted passage where extracted.

    Anthocyanins: detailed mechanisms of action (reviewed 4 October 2026) · lines 88–88

    Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication. · supports · Cell-free spectrophotometric metal assays; multiple pH and compound/metal ratios; exact series not extracted. · source_derived_draft · unverified_draft

    **Iron binding and metal reduction are different reactions.** Purified cyanidin, C3G and standardized elderberry extracts chelated iron and reduced iron and copper in cell-free assays; copper-chelating activity was relatively low. Extract behavior varied and was not completely explained by pigment concentration. Binding a metal, changing its oxidation state and reducing intestinal absorption are three different endpoints. This does not establish removal of intracellular metals or protection from ferroptosis in people. [Mladěnka et al., 2016](https://doi.org/10.1016/j.phytol.2016.10.025).
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.