Component
Intestinal PLP hydrolysis
Dephosphorylation of luminal PLP.
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What it acts on
Rat jejunal perfusion and in-vitro assays demonstrated phosphatase-mediated hydrolysis of luminal PLP.
Experimental context and source evidence
- evidence_location
- Indexed abstract: hydrolysis, albumin and pH experiments
- experimental_model
- Rat jejunal perfusion and in-vitro phosphatase assays.
- exposure
- PLP hydrolysis tested across luminal pH and albumin concentrations.
- limitations
- Luminal disappearance is not a complete absorption balance; no specific human phosphatase is assigned.
- nutrient_topic
- Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect. · Vitamin B6
- organism
- Rattus norvegicus
- plain_language
- Intestinal phosphatases remove PLP phosphate.
- primary_references
- [middleton1986] Intestinal hydrolysis of pyridoxal 5'-phosphate in vitro and in vivo in the rat. Effect of protein binding and pH. (1986). https://pubmed.ncbi.nlm.nih.gov/3721120/ DOI: 10.1016/0016-5085(86)90567-6
- tissue_or_cell_type
- Jejunum
Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 72–83
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Rat jejunal perfusion and in-vitro phosphatase assays. · source_derived_draft · unverified_draft
### b6-transport-gut-plp-hydrolysis Rat jejunal perfusion and in-vitro assays demonstrated phosphatase-mediated hydrolysis of luminal PLP. Condition category: normal nutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect. plain_language: Intestinal phosphatases remove PLP phosphate. organism: Rattus norvegicus tissue_or_cell_type: Jejunum experimental_model: Rat jejunal perfusion and in-vitro phosphatase assays. limitations: Luminal disappearance is not a complete absorption balance; no specific human phosphatase is assigned. exposure: PLP hydrolysis tested across luminal pH and albumin concentrations. evidence_location: Indexed abstract: hydrolysis, albumin and pH experiments [middleton1986] Intestinal hydrolysis of pyridoxal 5'-phosphate in vitro and in vivo in the rat. Effect of protein binding and pH. (1986). https://pubmed.ncbi.nlm.nih.gov/3721120/ DOI: 10.1016/0016-5085(86)90567-6
Complete structured claim and evidence
What acts on it
Albumin binding inhibited hydrolysis of 2 micromolar PLP at pH 5-7.4; at pH 3-4 binding was negligible and hydrolysis persisted.
Experimental context and source evidence
- evidence_location
- Indexed abstract: hydrolysis, albumin and pH experiments
- experimental_model
- Rat jejunal perfusion and in-vitro phosphatase assays.
- exposure
- 2 micromolar PLP, albumin and pH manipulation.
- limitations
- Albumin species is not assigned from the indexed abstract; no human acid-suppression outcome was measured.
- nutrient_topic
- Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect. · Vitamin B6
- organism
- Rattus norvegicus
- plain_language
- Protein binding and acidity changed substrate access.
- primary_references
- [middleton1986] Intestinal hydrolysis of pyridoxal 5'-phosphate in vitro and in vivo in the rat. Effect of protein binding and pH. (1986). https://pubmed.ncbi.nlm.nih.gov/3721120/ DOI: 10.1016/0016-5085(86)90567-6
- tissue_or_cell_type
- Perfused jejunum and in-vitro preparations
Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 85–96
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Rat jejunal perfusion and in-vitro phosphatase assays. · source_derived_draft · unverified_draft
### b6-transport-albumin-gut-hydrolysis Albumin binding inhibited hydrolysis of 2 micromolar PLP at pH 5-7.4; at pH 3-4 binding was negligible and hydrolysis persisted. Condition category: normal nutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect. plain_language: Protein binding and acidity changed substrate access. organism: Rattus norvegicus tissue_or_cell_type: Perfused jejunum and in-vitro preparations experimental_model: Rat jejunal perfusion and in-vitro phosphatase assays. limitations: Albumin species is not assigned from the indexed abstract; no human acid-suppression outcome was measured. exposure: 2 micromolar PLP, albumin and pH manipulation. evidence_location: Indexed abstract: hydrolysis, albumin and pH experiments [middleton1986] Intestinal hydrolysis of pyridoxal 5'-phosphate in vitro and in vivo in the rat. Effect of protein binding and pH. (1986). https://pubmed.ncbi.nlm.nih.gov/3721120/ DOI: 10.1016/0016-5085(86)90567-6
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.