Component
Human TG five-donor Y-to-F mutant
Y2540F/Y2766F/Y108F/Y234F/Y149F recombinant human TG.
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What it acts on
Replacing human TG donor residues Y2540, Y2766, Y108, Y234 and Y149 with phenylalanine suppressed significant T4 synthesis.
Experimental context and source evidence
- cross_nutrient
- false
- evidence_span
- {"source_cache": "artifacts/iodine-synthesis-sources/32025030.txt", "start_char": 9120, "end_char": 9940, "text_sha256": "8b8cd3eb09e3950bb40977268ed2a95e242386b632a9d45ccd8a2e156aead2b1", "text_characters": 820, "note": "Exact publisher passage retained in the cited local source cache; locator and digest supplied here."}
- experimental_model
- Human TG cryo-EM, recombinant HEK293T-expressed TG and site-directed mutants
- exposure
- 0.1 micromolar human TG, 1 mM KI, 24 mM glucose, 2 micrograms/mL glucose oxidase and 3 micrograms/mL lactoperoxidase; 10 minutes at 37 C followed by approximately 2.5 micrograms/mL Pronase and T4 ELISA. Most assays used lactoperoxidase after comparison with human TPO. Five donor substitutions.
- limitations
- Multi-site mutagenesis supports the donor set; not an isolated rate for every individual site.
- nutrient_topic
- Iodine research collection; topical membership is not evidence of a direct dietary effect. · Iodine
- organism
- Homo sapiens TG
- plain_language
- The paired donor tyrosines are also needed to assemble T4.
- primary_references
- [iodine-syn-tg2020] The structure of human thyroglobulin. (2020). https://pubmed.ncbi.nlm.nih.gov/32025030/ DOI: 10.1038/s41586-020-1995-4
- tissue_or_cell_type
- Purified-protein reaction
Iodine: thyroid hormone production, deficiency, excess and nutrient interactions (2026-09-17) · lines 646–658
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Human TG cryo-EM, recombinant HEK293T-expressed TG and site-directed mutants · source_derived_draft · unverified_draft
### iodine-syn-tg-donor-mutants Replacing human TG donor residues Y2540, Y2766, Y108, Y234 and Y149 with phenylalanine suppressed significant T4 synthesis. Condition category: normal nutrient_topic: Iodine research collection; topical membership is not evidence of a direct dietary effect. plain_language: The paired donor tyrosines are also needed to assemble T4. organism: Homo sapiens TG tissue_or_cell_type: Purified-protein reaction experimental_model: Human TG cryo-EM, recombinant HEK293T-expressed TG and site-directed mutants limitations: Multi-site mutagenesis supports the donor set; not an isolated rate for every individual site. exposure: 0.1 micromolar human TG, 1 mM KI, 24 mM glucose, 2 micrograms/mL glucose oxidase and 3 micrograms/mL lactoperoxidase; 10 minutes at 37 C followed by approximately 2.5 micrograms/mL Pronase and T4 ELISA. Most assays used lactoperoxidase after comparison with human TPO. Five donor substitutions. cross_nutrient: false evidence_span: {"source_cache": "artifacts/iodine-synthesis-sources/32025030.txt", "start_char": 9120, "end_char": 9940, "text_sha256": "8b8cd3eb09e3950bb40977268ed2a95e242386b632a9d45ccd8a2e156aead2b1", "text_characters": 820, "note": "Exact publisher passage retained in the cited local source cache; locator and digest supplied here."} [iodine-syn-tg2020] The structure of human thyroglobulin. (2020). https://pubmed.ncbi.nlm.nih.gov/32025030/ DOI: 10.1038/s41586-020-1995-4
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.