Component
Human SDHAF2 knockout state
Experimental biallelic SDHAF2 disruption in MDA-MB-231 breast cancer cells.
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What it acts on
SDHAF2 knockout and independent siRNA knockdown in MDA-MB-231 cells retained SDHA flavination; knockout cells also retained measurable SDH and succinate-quinone reductase activity.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- evidence_spans
- [{"source_bundle": "artifacts/riboflavin_metabolism_sources.json", "source_key": "PMC5076811", "locator": "HTML article p", "paragraph_index": 20, "char_start": 0, "char_end": 1274, "evidence_access": "full-text"}]
- experimental_model
- Human MDA-MB-231 breast cancer cells with CRISPR SDHAF2 disruption and independent siRNA knockdown.
- exposure
- CRISPR knockout, two independent siRNAs; FAD fluorescence/antibody and enzyme activity.
- limitations
- Cell-line-specific redundancy is not proof that SDHAF2 is dispensable in every tissue; contextual boundary to yeast and tumor findings.
- nutrient_topic
- Riboflavin research collection; topical membership is not evidence of a direct dietary effect. · Riboflavin (vitamin B2)
- organism
- Homo sapiens
- plain_language
- This breast cancer cell model assembled active, flavinated complex II without SDHAF2.
- primary_references
- [bezawork-geleta-2016-sdhaf2] The Assembly Factor SDHAF2 Is Dispensable for Flavination of the Catalytic Subunit of Mitochondrial Complex II in Breast Cancer Cells (2016). https://pubmed.ncbi.nlm.nih.gov/27587393/ DOI: 10.1074/jbc.c116.755017
- tissue_or_cell_type
- MDA-MB-231 breast cancer cells
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Riboflavin: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 715–726
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Human MDA-MB-231 breast cancer cells with CRISPR SDHAF2 disruption and independent siRNA knockdown. · source_derived_draft · unverified_draft
### b2-met-sdhaf2-knockout-context-boundary SDHAF2 knockout and independent siRNA knockdown in MDA-MB-231 cells retained SDHA flavination; knockout cells also retained measurable SDH and succinate-quinone reductase activity. Condition category: machinery_impairment nutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect. plain_language: This breast cancer cell model assembled active, flavinated complex II without SDHAF2. organism: Homo sapiens tissue_or_cell_type: MDA-MB-231 breast cancer cells experimental_model: Human MDA-MB-231 breast cancer cells with CRISPR SDHAF2 disruption and independent siRNA knockdown. limitations: Cell-line-specific redundancy is not proof that SDHAF2 is dispensable in every tissue; contextual boundary to yeast and tumor findings. exposure: CRISPR knockout, two independent siRNAs; FAD fluorescence/antibody and enzyme activity. evidence_spans: [{"source_bundle": "artifacts/riboflavin_metabolism_sources.json", "source_key": "PMC5076811", "locator": "HTML article p", "paragraph_index": 20, "char_start": 0, "char_end": 1274, "evidence_access": "full-text"}] [bezawork-geleta-2016-sdhaf2] The Assembly Factor SDHAF2 Is Dispensable for Flavination of the Catalytic Subunit of Mitochondrial Complex II in Breast Cancer Cells (2016). https://pubmed.ncbi.nlm.nih.gov/27587393/ DOI: 10.1074/jbc.c116.755017
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.