Component
Human RPE65 E148Q experimental substitution
Human RPE65 E148Q experimental substitution. Species, exposure and limitations are retained in each linked claim.
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What it acts on
RPE65 E148Q substitution did not reduce meso-zeaxanthin yield in the reported cultured-cell experiments.
Experimental context and source evidence
- evidence_span
- {"source_cache": "artifacts/lutein-research/41415389.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "b4eb5b9290310947be962df7dae42190e5afa6ffd726e869cb842d7f7ed15a41", "start_char": 0, "end_char": 1304, "text_sha256": "b4eb5b9290310947be962df7dae42190e5afa6ffd726e869cb842d7f7ed15a41"}
- experimental_model
- Cultured-cell enzyme expression, mutagenesis and structural modeling
- exposure
- Lutein substrate; E148Q substitution; binding-protein coexpression
- limitations
- Proposed radical transition state remains a mechanistic interpretation, not directly observed chemistry.
- nutrient_topic
- Lutein research collection; topical membership is not evidence of a direct dietary effect. · Lutein
- organism
- Human, chicken and mouse RPE65
- plain_language
- A change important to other RPE65 chemistry spared this measured reaction.
- primary_references
- [lutein-p41415389] Mechanism of Lutein to meso-Zeaxanthin Isomerization by RPE65 Catalysis. (2025). https://pubmed.ncbi.nlm.nih.gov/41415389/ DOI: 10.64898/2025.12.10.693550
- tissue_or_cell_type
- Cultured cells and molecular models
Lutein: metabolism, signaling and nutrient connections (2026-09-17) · lines 203–214
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Cultured-cell enzyme expression, mutagenesis and structural modeling · source_derived_draft · unverified_draft
### lutein-e148q-maintained RPE65 E148Q substitution did not reduce meso-zeaxanthin yield in the reported cultured-cell experiments. Condition category: normal nutrient_topic: Lutein research collection; topical membership is not evidence of a direct dietary effect. plain_language: A change important to other RPE65 chemistry spared this measured reaction. organism: Human, chicken and mouse RPE65 tissue_or_cell_type: Cultured cells and molecular models experimental_model: Cultured-cell enzyme expression, mutagenesis and structural modeling limitations: Proposed radical transition state remains a mechanistic interpretation, not directly observed chemistry. exposure: Lutein substrate; E148Q substitution; binding-protein coexpression evidence_span: {"source_cache": "artifacts/lutein-research/41415389.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "b4eb5b9290310947be962df7dae42190e5afa6ffd726e869cb842d7f7ed15a41", "start_char": 0, "end_char": 1304, "text_sha256": "b4eb5b9290310947be962df7dae42190e5afa6ffd726e869cb842d7f7ed15a41"} [lutein-p41415389] Mechanism of Lutein to meso-Zeaxanthin Isomerization by RPE65 Catalysis. (2025). https://pubmed.ncbi.nlm.nih.gov/41415389/ DOI: 10.64898/2025.12.10.693550
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.